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Article

Effects of FOXM1 inhibition and ionizing radiation on melanoma cells

  • Authors:
    • Vivienne S. Lee
    • Lucinda S. McRobb
    • Vaughan Moutrie
    • Estavam D. Santos
    • Timothy L. Siu
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Medicine, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, NSW 2109, Australia, Genesis Cancer Care, Macquarie University Hospital, Sydney, NSW 2109, Australia
  • Pages: 6822-6830
    |
    Published online on: September 21, 2018
       https://doi.org/10.3892/ol.2018.9482
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Abstract

Metastatic melanoma can be highly refractory to conventional radiotherapy and chemotherapy but combinatorial‑targeted therapeutics are showing greater promise on improving treatment efficacy. Previous studies have shown that knockdown of Forkhead box M1 (FOXM1) can sensitize various tumor types to radiation‑induced cell death. The effect of combining radiation with a small molecule FOXM1 inhibitor, Siomycin A, on growth, death and migration of a metastatic melanoma cell line (SK‑MEL‑28) that overexpresses this pleiotropic cell cycle regulator was investigated. Siomycin A (SIOA) was found to be a strong inducer of apoptosis, and inhibitor of proliferation and migration in a scratch wound assay in this cell line. Induction of apoptosis occurred at concentrations >1 µM in association with reductions in the constitutive FOXM1 and anti‑apoptotic B‑cell lymphoma 2 protein levels found in these cells. Single doses of ionizing radiation (0‑40 Gy) delivered by linear accelerator caused inhibition of growth and migration without significant induction of cell death. Pretreatment with SIOA did not increase the sensitivity of this melanoma cell line to radiation as observed in other tumor types. These data confirm that as a single agent, SIOA is an effective inducer of cell death and inhibitor of migration in metastatic melanoma cells expressing constitutive FOXM1. In combination with radiation, SIOA pre‑treatment, however, may not be of added benefit.
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Copy and paste a formatted citation
Spandidos Publications style
Lee VS, McRobb LS, Moutrie V, Santos ED and Siu TL: Effects of FOXM1 inhibition and ionizing radiation on melanoma cells. Oncol Lett 16: 6822-6830, 2018.
APA
Lee, V.S., McRobb, L.S., Moutrie, V., Santos, E.D., & Siu, T.L. (2018). Effects of FOXM1 inhibition and ionizing radiation on melanoma cells. Oncology Letters, 16, 6822-6830. https://doi.org/10.3892/ol.2018.9482
MLA
Lee, V. S., McRobb, L. S., Moutrie, V., Santos, E. D., Siu, T. L."Effects of FOXM1 inhibition and ionizing radiation on melanoma cells". Oncology Letters 16.5 (2018): 6822-6830.
Chicago
Lee, V. S., McRobb, L. S., Moutrie, V., Santos, E. D., Siu, T. L."Effects of FOXM1 inhibition and ionizing radiation on melanoma cells". Oncology Letters 16, no. 5 (2018): 6822-6830. https://doi.org/10.3892/ol.2018.9482
Copy and paste a formatted citation
x
Spandidos Publications style
Lee VS, McRobb LS, Moutrie V, Santos ED and Siu TL: Effects of FOXM1 inhibition and ionizing radiation on melanoma cells. Oncol Lett 16: 6822-6830, 2018.
APA
Lee, V.S., McRobb, L.S., Moutrie, V., Santos, E.D., & Siu, T.L. (2018). Effects of FOXM1 inhibition and ionizing radiation on melanoma cells. Oncology Letters, 16, 6822-6830. https://doi.org/10.3892/ol.2018.9482
MLA
Lee, V. S., McRobb, L. S., Moutrie, V., Santos, E. D., Siu, T. L."Effects of FOXM1 inhibition and ionizing radiation on melanoma cells". Oncology Letters 16.5 (2018): 6822-6830.
Chicago
Lee, V. S., McRobb, L. S., Moutrie, V., Santos, E. D., Siu, T. L."Effects of FOXM1 inhibition and ionizing radiation on melanoma cells". Oncology Letters 16, no. 5 (2018): 6822-6830. https://doi.org/10.3892/ol.2018.9482
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