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Article

Anticancer effects of curcumin on nude mice bearing lung cancer A549 cell subsets SP and NSP cells

  • Authors:
    • Xiaojiang Li
    • Shaojun Ma
    • Peiying Yang
    • Binxu Sun
    • Ying Zhang
    • Yuehong Sun
    • Meimei Hao
    • Ruiyu Mou
    • Yingjie Jia
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, P.R. China, Department of Interventional Vascular, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, P.R. China, Department of Radiochemotherapy, Cangzhou Hospital of Integrated Traditional Chinese Medicine and Western Medicine, Cangzhou, Hebei 061899, P.R. China, Department of Oncology, Shanxi Provincial Hospital of Traditional Chinese Medicine, Taiyuan, Shanxi 710003, P.R. China
  • Pages: 6756-6762
    |
    Published online on: September 24, 2018
       https://doi.org/10.3892/ol.2018.9488
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Abstract

Curcumin is a key polyphenolic curcuminoid extracted from the root of turmeric rhizome Curcuma longa Linn, which is a frequently used Chinese herb for the treatment of cancer. The aim of the present study was to investigate the mechanism of the inhibitory effects of curcumin on nude mice with lung cancer A549 cell subsets side population (SP) and non‑SP (NSP) cells. BALB/c mice were subcutaneously injected with the tumor cells of A549 SP or NSP subsets consisting of 1x109 cells/l (0.2 ml in total). After 16 days of inoculation with A549, the mice were intraperitoneally injected with curcumin (100 mg/kg, 0.2 ml) once every other day, eight times in total. A series of assays were performed to detect the effects of curcumin on: i) Tumor weight and size; ii) Notch and hypoxia inducible factor 1 (HIF‑1) mRNA expression by quantitative polymerase chain reaction; and iii) vascular endothelial growth factor (VEGF) and nuclear factor‑κB (NF‑κB) by immunohistochemistry. It was determined that curcumin decreased the tumor weight and size, downregulated the expression of Notch and HIF‑1 mRNA and suppressed the VEGF and NF‑κB expression. These results indicated that curcumin inhibited lung cancer growth through the regulation of angiogenesis mediated by VEGF signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Ma S, Yang P, Sun B, Zhang Y, Sun Y, Hao M, Mou R and Jia Y: Anticancer effects of curcumin on nude mice bearing lung cancer A549 cell subsets SP and NSP cells. Oncol Lett 16: 6756-6762, 2018.
APA
Li, X., Ma, S., Yang, P., Sun, B., Zhang, Y., Sun, Y. ... Jia, Y. (2018). Anticancer effects of curcumin on nude mice bearing lung cancer A549 cell subsets SP and NSP cells. Oncology Letters, 16, 6756-6762. https://doi.org/10.3892/ol.2018.9488
MLA
Li, X., Ma, S., Yang, P., Sun, B., Zhang, Y., Sun, Y., Hao, M., Mou, R., Jia, Y."Anticancer effects of curcumin on nude mice bearing lung cancer A549 cell subsets SP and NSP cells". Oncology Letters 16.5 (2018): 6756-6762.
Chicago
Li, X., Ma, S., Yang, P., Sun, B., Zhang, Y., Sun, Y., Hao, M., Mou, R., Jia, Y."Anticancer effects of curcumin on nude mice bearing lung cancer A549 cell subsets SP and NSP cells". Oncology Letters 16, no. 5 (2018): 6756-6762. https://doi.org/10.3892/ol.2018.9488
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Ma S, Yang P, Sun B, Zhang Y, Sun Y, Hao M, Mou R and Jia Y: Anticancer effects of curcumin on nude mice bearing lung cancer A549 cell subsets SP and NSP cells. Oncol Lett 16: 6756-6762, 2018.
APA
Li, X., Ma, S., Yang, P., Sun, B., Zhang, Y., Sun, Y. ... Jia, Y. (2018). Anticancer effects of curcumin on nude mice bearing lung cancer A549 cell subsets SP and NSP cells. Oncology Letters, 16, 6756-6762. https://doi.org/10.3892/ol.2018.9488
MLA
Li, X., Ma, S., Yang, P., Sun, B., Zhang, Y., Sun, Y., Hao, M., Mou, R., Jia, Y."Anticancer effects of curcumin on nude mice bearing lung cancer A549 cell subsets SP and NSP cells". Oncology Letters 16.5 (2018): 6756-6762.
Chicago
Li, X., Ma, S., Yang, P., Sun, B., Zhang, Y., Sun, Y., Hao, M., Mou, R., Jia, Y."Anticancer effects of curcumin on nude mice bearing lung cancer A549 cell subsets SP and NSP cells". Oncology Letters 16, no. 5 (2018): 6756-6762. https://doi.org/10.3892/ol.2018.9488
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