miR‑218 suppresses the proliferation of osteosarcoma through downregulation of E2F2

Xuan,Chengmin; Jin,Mingwei; Gao,Yong; Xu,Shumei; Wang,Lei; Wang,Yuan; Han,Rui; An,Qi

doi:10.3892/ol.2018.9576

miR‑218 suppresses the proliferation of osteosarcoma through downregulation of E2F2

Authors:
- Chengmin Xuan
- Mingwei Jin
- Yong Gao
- Shumei Xu
- Lei Wang
- Yuan Wang
- Rui Han
- Qi An
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Affiliations: Department of Hematology, Xuzhou Children's Hospital, Xuzhou, Jiangsu 221006, P.R. China, Department of Clinical Medicine, The Graduate School, Xuzhou Medical College, Xuzhou, Jiangsu 221004, P.R. China
Published online on: October 15, 2018 https://doi.org/10.3892/ol.2018.9576
Pages: 571-577

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Abstract

Osteosarcoma is the most common primary malignant bone tumor type in children and adolescents under 20 years of age. Biological characteristics include invasiveness, metastasis, abnormal differentiation and loss of contact inhibition. microRNAs (miRNAs) are involved in the transcriptional and post‑transcriptional regulation of target mRNAs. Previous studies have demonstrated that miR‑218 inhibits tumor formation and progression in glioma, colon cancer and renal cell carcinoma; however, the mechanism of action of miR‑218 in osteosarcoma has not been completely determined. In the present study, it was demonstrated that miR‑218 exhibited low expression and targeted E2F2 in osteosarcoma cells. Additionally, overexpression of miR‑218 inhibited osteosarcoma cell proliferation, with the opposite result occurring following the knockdown of miR‑218. Furthermore, it was determined that miR‑218 inhibited tumor formation and reduced the expression of E2F2 and proliferating cell nuclear antigen in nude mice. Collectively, the present data demonstrated that miR‑218 serves an important role in suppressing the proliferation of osteosarcoma cells, potentially regulated by E2F2, which may provide a novel protein marker for the treatment of osteosarcoma.

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