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Article

Silencing of miR‑19a‑3p enhances osteosarcoma cells chemosensitivity by elevating the expression of tumor suppressor PTEN

  • Authors:
    • Bo Zhang
    • Yuan Liu
    • Jiangnan Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, The First People's Hospital of Wenling, Wenling, Zhejiang 317500, P.R. China, Department of Medical Examination, Center for Disease Control and Prevention of Wenling, Wenling, Zhejiang 317500, P.R. China
  • Pages: 414-421
    |
    Published online on: October 17, 2018
       https://doi.org/10.3892/ol.2018.9592
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Abstract

MicroRNAs (miRNAs/miRs) are small non‑coding RNAs, which serve important roles in tumor progression. The present study analyzed the role of miR‑19a‑3p in the chemosensitivity of osteosarcoma (OS) cells. Overexpression of miR‑19a‑3p was observed in OS cells and a cisplatin‑resistant MG63 cell line was subsequently constructed. It was observed that miR‑19a‑3p inhibitor transfection suppressed cell proliferation and decreased the expression of Ki67 and PCNA compared with the cisplatin treatment group. miR‑19a‑3p inhibitor transfection also promoted apoptotic rate, increased the expression of Bcl‑2 associated X, apoptosis regulator (Bax) and markedly decreased the expression of Bcl‑2 compared with the cisplatin treatment group. These results elucidated that silencing of miR‑19a‑3p enhanced chemosensitivity of OS cells to Cisplatin, through suppressing cell proliferation and promoting cell apoptosis during treatment with Cisplatin. Bioinformatics study and luciferase reporter assays indicated that PTEN was a target of miR‑19a‑3p, and western blotting demonstrated that PTEN expression was negatively regulated by miR‑19a‑3p in OS cells. In addition, overexpression of PTEN decreased cell proliferation, but increased apoptotic rate compared with the cisplatin treatment group. It was observed that inhibition of PTEN by BpV(HOpic) upregulated cell proliferation and downregulated apoptotic rate compared with the Cisplatin‑treated miR‑19a‑3p inhibitor group, indicating that inhibition of PTEN expression counteracted the effect of the miR‑19a‑3p inhibitor on the regulation of chemosensitivity in OS cells. Taken together, overexpression of miR‑19a‑3p was observed in OS cell lines and that downregulation of miR‑19a‑3p enhanced the chemosensitivity of OS cells to Cisplatin, by elevating the expression of the tumor suppressor, PTEN.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang B, Liu Y and Zhang J: Silencing of miR‑19a‑3p enhances osteosarcoma cells chemosensitivity by elevating the expression of tumor suppressor PTEN. Oncol Lett 17: 414-421, 2019.
APA
Zhang, B., Liu, Y., & Zhang, J. (2019). Silencing of miR‑19a‑3p enhances osteosarcoma cells chemosensitivity by elevating the expression of tumor suppressor PTEN. Oncology Letters, 17, 414-421. https://doi.org/10.3892/ol.2018.9592
MLA
Zhang, B., Liu, Y., Zhang, J."Silencing of miR‑19a‑3p enhances osteosarcoma cells chemosensitivity by elevating the expression of tumor suppressor PTEN". Oncology Letters 17.1 (2019): 414-421.
Chicago
Zhang, B., Liu, Y., Zhang, J."Silencing of miR‑19a‑3p enhances osteosarcoma cells chemosensitivity by elevating the expression of tumor suppressor PTEN". Oncology Letters 17, no. 1 (2019): 414-421. https://doi.org/10.3892/ol.2018.9592
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang B, Liu Y and Zhang J: Silencing of miR‑19a‑3p enhances osteosarcoma cells chemosensitivity by elevating the expression of tumor suppressor PTEN. Oncol Lett 17: 414-421, 2019.
APA
Zhang, B., Liu, Y., & Zhang, J. (2019). Silencing of miR‑19a‑3p enhances osteosarcoma cells chemosensitivity by elevating the expression of tumor suppressor PTEN. Oncology Letters, 17, 414-421. https://doi.org/10.3892/ol.2018.9592
MLA
Zhang, B., Liu, Y., Zhang, J."Silencing of miR‑19a‑3p enhances osteosarcoma cells chemosensitivity by elevating the expression of tumor suppressor PTEN". Oncology Letters 17.1 (2019): 414-421.
Chicago
Zhang, B., Liu, Y., Zhang, J."Silencing of miR‑19a‑3p enhances osteosarcoma cells chemosensitivity by elevating the expression of tumor suppressor PTEN". Oncology Letters 17, no. 1 (2019): 414-421. https://doi.org/10.3892/ol.2018.9592
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