Analysis of the inhibitory effects of miR‑124 and miR-152 on human epithelial ovarian cancer xenografts in a nude mouse model

Liu,Weiwei; Zhang,Lixia; Wang,Jing; Wang,Xiaoli; Sun,Hong

doi:10.3892/ol.2018.9612

Analysis of the inhibitory effects of miR‑124 and miR-152 on human epithelial ovarian cancer xenografts in a nude mouse model

Authors:
- Weiwei Liu
- Lixia Zhang
- Jing Wang
- Xiaoli Wang
- Hong Sun
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Affiliations: Department of Gynecology, Yidu Central Hospital of Weifang, Weifang, Shandong 262500, P.R. China, Department of Gynecology, The Second People's Hospital of Dezhou, Dezhou, Shandong 253000, P.R. China
Published online on: October 24, 2018 https://doi.org/10.3892/ol.2018.9612
Pages: 348-354
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

This study investigated the inhibitory effects of miR-124 and miR-152 on the growth of human ovarian cancer (OC) SKOV3 cell line subcutaneous xenografts in nude mice. Twenty-eight healthy nude mice were selected and divided into the experimental group 1 (n=4), experimental group 2 (n=4), negative control group 1 (n=4), negative control group 2 (n=4), blank control group 1 (n=4), blank control group 2 (n=4) and observation group (n=4) according to the principle of similarity in body weight. The transfected SKOV3 cells were inoculated subcutaneously into the nape of the nude mice. After tumorigenesis, miR-124 mimics, miR-152 mimics, and their negative controls were transiently transfected into human OC SKOV3 cells via lipofection method. The expression levels of miR-124 and miR-152 were detected via reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and those of Ki-67 and caspase-3 were detected by western blotting. After transfection, the expression levels of miR-124 and miR-152 in the SKOV3 cells were significantly upregulated. The nude mice were sacrificed 36 days later, and tumor nodes of nude mice transfected with miR-124 and miR-152 grew slowly. Compared with that in the experimental groups, tumor size in the blank control and negative control groups was gradually increased with the increment of days (P<0.05). The volume of subcutaneous xenografts in nude mice of miR-124 and miR-152 experimental groups was obviously smaller than that in the blank control and negative control groups (P<0.05). Besides, the inhibition of tumor size in the observation group was more significant than that in the experimental groups (P<0.05). Thus, miR-124 and miR-152 inhibit the growth of human epithelial OC xenografts in nude mice, and they are expected to become new targets for gene‑based therapy of OC.

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