Open Access

Basic transcription factor 3 expression silencing attenuates colon cancer cell proliferation and migration in vitro

  • Authors:
    • Xu Li
    • Jinke Sui
    • Junjie Xing
    • Fuao Cao
    • Hao Wang
    • Chuangang Fu
    • Hantao Wang
  • View Affiliations

  • Published online on: October 24, 2018     https://doi.org/10.3892/ol.2018.9613
  • Pages: 113-118
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Basic transcription factor 3 (BTF3) is an RNA polymerase II transcription factor that also regulates apoptosis. Numerous studies have identified that BTF3 is aberrantly expressed in several types of tumor. However, the function of BTF3 in colorectal cancer remains unknown. The aim of the present study was to assess the function of BTF3 during colon cancer tumorigenesis. Applying a lentivirus‑transfected short hairpin RNA approach, expression of BTF3 was dysregulated in the colon cancer HCT116 and HT‑29 cell lines; knockdown efficiency was verified using the quantitative polymerase chain reaction and western blotting. To determine the function of BTF3 in colon cancer, cell proliferation was assessed using an MTT assay, cell apoptosis and the cell cycle were assessed using flow cytometry, and cell migration was assessed using a Transwell assay. Knockdown of BTF3 inhibited cell proliferation, possibly because BTF3 knockdown induced cell early apoptosis and arrested cells in G0‑G1 phase. BTF3 knockdown also inhibited cell migration. The results of the present study identified that BTF3 expression is associated with colon cancer progress, and BTF3 may therefore be a molecular marker for diagnosis and treatment outcomes of human colon cancer.
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January-2019
Volume 17 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Li X, Sui J, Xing J, Cao F, Wang H, Fu C and Wang H: Basic transcription factor 3 expression silencing attenuates colon cancer cell proliferation and migration in vitro. Oncol Lett 17: 113-118, 2019
APA
Li, X., Sui, J., Xing, J., Cao, F., Wang, H., Fu, C., & Wang, H. (2019). Basic transcription factor 3 expression silencing attenuates colon cancer cell proliferation and migration in vitro. Oncology Letters, 17, 113-118. https://doi.org/10.3892/ol.2018.9613
MLA
Li, X., Sui, J., Xing, J., Cao, F., Wang, H., Fu, C., Wang, H."Basic transcription factor 3 expression silencing attenuates colon cancer cell proliferation and migration in vitro". Oncology Letters 17.1 (2019): 113-118.
Chicago
Li, X., Sui, J., Xing, J., Cao, F., Wang, H., Fu, C., Wang, H."Basic transcription factor 3 expression silencing attenuates colon cancer cell proliferation and migration in vitro". Oncology Letters 17, no. 1 (2019): 113-118. https://doi.org/10.3892/ol.2018.9613