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Cistanche tubulosa phenylethanoid glycosides induce apoptosis in Eca‑109 cells via the mitochondria‑dependent pathway

  • Authors:
    • Changshuang Fu
    • Jinyu Li
    • Adila Aipire
    • Lijie Xia
    • Yi Yang
    • Qiuyan Chen
    • Jie Lv
    • Xinhui Wang
    • Jinyao Li
  • View Affiliations / Copyright

    Affiliations: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang 830046, P.R. China, College of Life Science, Xinjiang Normal University, Urumqi, Xinjiang 830054, P.R. China, College of Resource and Environment Sciences, Xinjiang University, Urumqi, Xinjiang 830046, P.R. China
    Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 303-313
    |
    Published online on: October 29, 2018
       https://doi.org/10.3892/ol.2018.9635
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Abstract

Cistanche tubulosa has various biological functions. In the present study, the antitumor effect of water‑soluble phenylethanoid glycosides of C. tubulosa (CTPG‑W) on esophageal cancer was investigated. Eca‑109 cells were treated with CTPG‑W and the cell viability was measured by MTT assay. The apoptosis, cell cycle, mitochondrial membrane potential (Δψm) and reactive oxygen species were analyzed by flow cytometry. The levels of proteins in apoptotic pathways were detected by western blot analysis. It was determined that CTPG‑W significantly reduced the viability of Eca‑109 cells through the induction of apoptosis and cell cycle arrest. Following CTPG‑W treatment, the Δψm of Eca‑109 was notably decreased, which is associated with the upregulated levels of B‑cell lymphoma‑2 (Bcl‑2)‑associated X and downregulated levels of Bcl‑2. Consequently, the levels of cytochrome c and c‑Jun NH2‑terminal kinase were increased, which upregulated the levels of cleaved‑poly (ADP‑ribose) polymerase and cleaved‑caspase‑3, ‑7 and ‑9, but not caspase‑8. Correspondingly, the levels of reactive oxygen species in Eca‑109 cells demonstrated notable changes. These results indicated that CTPG‑W induced apoptosis of Eca‑109 cells through a mitochondrial‑dependent pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Fu C, Li J, Aipire A, Xia L, Yang Y, Chen Q, Lv J, Wang X and Li J: Cistanche tubulosa phenylethanoid glycosides induce apoptosis in Eca‑109 cells via the mitochondria‑dependent pathway. Oncol Lett 17: 303-313, 2019.
APA
Fu, C., Li, J., Aipire, A., Xia, L., Yang, Y., Chen, Q. ... Li, J. (2019). Cistanche tubulosa phenylethanoid glycosides induce apoptosis in Eca‑109 cells via the mitochondria‑dependent pathway. Oncology Letters, 17, 303-313. https://doi.org/10.3892/ol.2018.9635
MLA
Fu, C., Li, J., Aipire, A., Xia, L., Yang, Y., Chen, Q., Lv, J., Wang, X., Li, J."Cistanche tubulosa phenylethanoid glycosides induce apoptosis in Eca‑109 cells via the mitochondria‑dependent pathway". Oncology Letters 17.1 (2019): 303-313.
Chicago
Fu, C., Li, J., Aipire, A., Xia, L., Yang, Y., Chen, Q., Lv, J., Wang, X., Li, J."Cistanche tubulosa phenylethanoid glycosides induce apoptosis in Eca‑109 cells via the mitochondria‑dependent pathway". Oncology Letters 17, no. 1 (2019): 303-313. https://doi.org/10.3892/ol.2018.9635
Copy and paste a formatted citation
x
Spandidos Publications style
Fu C, Li J, Aipire A, Xia L, Yang Y, Chen Q, Lv J, Wang X and Li J: Cistanche tubulosa phenylethanoid glycosides induce apoptosis in Eca‑109 cells via the mitochondria‑dependent pathway. Oncol Lett 17: 303-313, 2019.
APA
Fu, C., Li, J., Aipire, A., Xia, L., Yang, Y., Chen, Q. ... Li, J. (2019). Cistanche tubulosa phenylethanoid glycosides induce apoptosis in Eca‑109 cells via the mitochondria‑dependent pathway. Oncology Letters, 17, 303-313. https://doi.org/10.3892/ol.2018.9635
MLA
Fu, C., Li, J., Aipire, A., Xia, L., Yang, Y., Chen, Q., Lv, J., Wang, X., Li, J."Cistanche tubulosa phenylethanoid glycosides induce apoptosis in Eca‑109 cells via the mitochondria‑dependent pathway". Oncology Letters 17.1 (2019): 303-313.
Chicago
Fu, C., Li, J., Aipire, A., Xia, L., Yang, Y., Chen, Q., Lv, J., Wang, X., Li, J."Cistanche tubulosa phenylethanoid glycosides induce apoptosis in Eca‑109 cells via the mitochondria‑dependent pathway". Oncology Letters 17, no. 1 (2019): 303-313. https://doi.org/10.3892/ol.2018.9635
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