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Association between NF-κB expression and drug resistance of liver cancer

  • Authors:
    • Tao Liu
    • Rendong Wei
    • Yiting Zhang
    • Wen Chen
    • Haidong Liu
  • View Affiliations / Copyright

    Affiliations: Department of Hepatology, The Sixth People's Hospital of Qingdao, Qingdao, Shandong 266033, P.R. China, Department of Infectious Diseases, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China, Department of Digestive Diseases, The Sixth People's Hospital of Qingdao, Qingdao, Shandong 266033, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1030-1034
    |
    Published online on: October 30, 2018
       https://doi.org/10.3892/ol.2018.9640
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Abstract

Association between the expression of nuclear factor κB (NF-κB) and the drug resistance of hepatoma cells was investigated. HepG-2 cells and HepG2/ADM cells were cultured, respectively. The morphology and status of the two groups of cells were observed by cell white light. The immuno­fluorescence by NF-κB and MDR1 staining on HepG-2 cells and HepG2/ADM cells, respectively, was applied and the fluorescence expression in the two groups of cells was observed. RT‑qPCR was used to detect the expression of NF-κB and MDR1 mRNA, the NF-κB and MDR1 protein expression was detected by western blot analysis. The results of cell white illumination showed that the structure of HepG-2 and HepG2/ADM cells was complete and the cell morphology was normal, and there was no significant difference, and could be used for comparative study. Immunofluorescence staining showed that the expression of NF-κB and MDR1 in HepG-2 cells was very low, while the expression of NF-κB and MDR1 in HepG2/ADM cells was increased significantly. The RT-qPCR results showed that NF-κB and MDR1 mRNA expression in HepG-2 cells was very low, while NF-κB and MDR1 mRNA expression in HepG-2/ADM cells was significantly increased, and western blot results showed that NF-κB and MDR1 protein expression in HepG-2 cells was very low, while NF-κB and MDR1 protein expression in HepG-2/ADM cells was increased significantly. The results of variance analysis showed that there was significant difference in the expression of the control group and paeonol group (P<0.01). In conclusion, the expression of NF-κB in the drug-resistant cells of liver cancer is closely related to the resistance‑related gene MDR1. This result may provide a new solution for the drug resistance of liver cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Liu T, Wei R, Zhang Y, Chen W and Liu H: Association between NF-κB expression and drug resistance of liver cancer. Oncol Lett 17: 1030-1034, 2019.
APA
Liu, T., Wei, R., Zhang, Y., Chen, W., & Liu, H. (2019). Association between NF-κB expression and drug resistance of liver cancer. Oncology Letters, 17, 1030-1034. https://doi.org/10.3892/ol.2018.9640
MLA
Liu, T., Wei, R., Zhang, Y., Chen, W., Liu, H."Association between NF-κB expression and drug resistance of liver cancer". Oncology Letters 17.1 (2019): 1030-1034.
Chicago
Liu, T., Wei, R., Zhang, Y., Chen, W., Liu, H."Association between NF-κB expression and drug resistance of liver cancer". Oncology Letters 17, no. 1 (2019): 1030-1034. https://doi.org/10.3892/ol.2018.9640
Copy and paste a formatted citation
x
Spandidos Publications style
Liu T, Wei R, Zhang Y, Chen W and Liu H: Association between NF-κB expression and drug resistance of liver cancer. Oncol Lett 17: 1030-1034, 2019.
APA
Liu, T., Wei, R., Zhang, Y., Chen, W., & Liu, H. (2019). Association between NF-κB expression and drug resistance of liver cancer. Oncology Letters, 17, 1030-1034. https://doi.org/10.3892/ol.2018.9640
MLA
Liu, T., Wei, R., Zhang, Y., Chen, W., Liu, H."Association between NF-κB expression and drug resistance of liver cancer". Oncology Letters 17.1 (2019): 1030-1034.
Chicago
Liu, T., Wei, R., Zhang, Y., Chen, W., Liu, H."Association between NF-κB expression and drug resistance of liver cancer". Oncology Letters 17, no. 1 (2019): 1030-1034. https://doi.org/10.3892/ol.2018.9640
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