Expression and clinical significance of basic transcription factor 3 in nasopharyngeal carcinoma

Chen,Ping; Zhong,Qi; Li,Zufei; Zhang,Yang; Huang,Zhigang

doi:10.3892/ol.2018.9699

Expression and clinical significance of basic transcription factor 3 in nasopharyngeal carcinoma

Authors:
- Ping Chen
- Qi Zhong
- Zufei Li
- Yang Zhang
- Zhigang Huang
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Affiliations: Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China
Published online on: November 14, 2018 https://doi.org/10.3892/ol.2018.9699
Pages: 789-796
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Basic transcription factor 3 (BTF3), a transcription factor and modulator of apoptosis, is differentially expressed in carcinoma. To acquire further understanding of the involvement of BTF3 in carcinoma, the present study analyzed the expression of BTF3, as well as its role in cell function in nasopharyngeal carcinoma (NPC). BTF3 transcription rates in human NPC samples (n=46) and adjacent normal tissue samples (n=46) were analyzed using reverse transcription‑quantitative polymerase chain reaction and immunohistochemistry. BTF3‑silencing in NPC cells was performed via specific small interfering RNA molecules. The function of BTF3 was analyzed by proliferation assays and colony forming assays using a Cellomic assay system. The positive expression rates of BTF3 were significantly increased in cancerous tissues compared with those in adjacent tissues (P<0.05). In addition, BTF3‑silencing decreased cell proliferation and colony formation (P<0.01) in TCA‑8113 and 5‑8F cells. BTF3 is overexpressed in NPC, and its silencing is associated with decreased cell proliferation and colony formation, enhanced apoptosis and cell cycle regulation of TCA‑8113 and 5‑8F cells.

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