miR‑410 acts as an oncogene in colorectal cancer cells by targeting dickkopf‑related protein 1 via the Wnt/β‑catenin signaling pathway

Wang,Wei; He,Ying; Rui,Jing; Xu,Mao‑Qi

doi:10.3892/ol.2018.9710

miR‑410 acts as an oncogene in colorectal cancer cells by targeting dickkopf‑related protein 1 via the Wnt/β‑catenin signaling pathway

Authors:
- Wei Wang
- Ying He
- Jing Rui
- Mao‑Qi Xu
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Affiliations: Department of General Surgery, Wuhu Hospital of Traditional Chinese Medicine, Wuhu, Anhui 241000, P.R. China, Department of Stomatology, The Second People's Hospital of Wuhu, Wuhu, Anhui 241000, P.R. China
Published online on: November 15, 2018 https://doi.org/10.3892/ol.2018.9710
Pages: 807-814
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Colorectal cancer (CRC) is a common malignancy with high morbidity. MicroRNAs (miRNAs or miRs) have been demonstrated to be critical post‑transcriptional regulators in tumorigenesis. The current study aimed to investigate the effect of miR‑410 on the proliferation and metastasis of CRC. The expression of miR‑410 was examined in CRC cell lines. SW‑480 and HCT‑116 CRC cell lines were employed and transfected with miR‑410 inhibitor or miR‑410 mimics. The association between miR‑410 and dickkopf‑related protein 1 (DKK‑1) was verified by luciferase reporter assay. Cell viability and apoptosis were detected by Cell Counting Kit‑8 (CCK‑8) and flow cytometry assay. Cell migration and invasion capacity were determined by Transwell assay. The protein level of DKK1, β‑catenin and phosphorylated glycogen synthase kinase‑3β (pGSK‑3β) were analyzed by western blotting. miR‑410 was revealed to be upregulated in CRC cell lines. Further studies identified DKK‑1 as a direct target of miR‑410. In addition, knockdown of miR‑410 promoted the expression of DKK, inhibited CRC cell proliferation, migration and invasion capacity, and induced cell apoptosis, while overexpression of miR‑410 reversed these results. miR‑410 silencing also decreased β‑catenin and pGSK‑3β levels. The current study indicated that miR‑410 negatively regulates the expression of DKK‑1 in vitro. miR‑410 promotes malignancy phenotypes in CRC cell lines. This regulatory effect of miR‑410 may be associated with the Wnt/β‑catenin signaling pathway. Therefore, miR‑410 could be used as a biomarker for predicting the progression of CRC.

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