miR‑885‑5p suppresses osteosarcoma proliferation, migration and invasion through regulation of β‑catenin

Liu,Yan; Bao,Zili; Tian,Wanqing; Huang,Guicheng

doi:10.3892/ol.2018.9768

miR‑885‑5p suppresses osteosarcoma proliferation, migration and invasion through regulation of β‑catenin

Authors:
- Yan Liu
- Zili Bao
- Wanqing Tian
- Guicheng Huang
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Affiliations: Department of Orthopedics, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China, Department of Orthopedics, Jingjiang Hospital of Chinese Medicine, Jingjiang, Jiangsu 214500, P.R. China
Published online on: November 28, 2018 https://doi.org/10.3892/ol.2018.9768
Pages: 1996-2004

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Abstract

MicroRNAs (miRs) have been reported to serve key roles in cancer. To investigate the function of miR‑885‑5p in osteosarcoma, the expression levels of miR‑885‑5p were analyzed in 85 osteosarcoma tissue samples and adjacent non‑cancerous tissue samples, using reverse transcription‑quantitative polymerase chain reaction analysis. It was demonstrated that miR‑885‑5p was downregulated in osteosarcoma tissues and cell lines. Notably, the expression level of miR‑885‑5p was closely associated with tumor size, Tumor‑Node‑Metastasis stage and lymph node metastasis. Additionally, low expression levels of miR‑885‑5p also predicted a poor prognosis of osteosarcoma. To further decipher the roles of miR‑885‑5p in osteosarcoma, it was determined that β‑catenin, a key component of the Wnt signaling pathway, was a target of miR‑885‑5p. Furthermore, several functional experiments, including a colony formation assay, CCK‑8 assay, wound healing assay and Transwell invasion assay, revealed that miR‑885‑5p suppressed cell proliferation, migration and invasion through inhibition of β‑catenin. The results of the present study provide a novel insight into the molecular roles of miR‑885‑5p in osteosarcoma.

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