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Article

miR‑885‑5p suppresses osteosarcoma proliferation, migration and invasion through regulation of β‑catenin

  • Authors:
    • Yan Liu
    • Zili Bao
    • Wanqing Tian
    • Guicheng Huang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China, Department of Orthopedics, Jingjiang Hospital of Chinese Medicine, Jingjiang, Jiangsu 214500, P.R. China
  • Pages: 1996-2004
    |
    Published online on: November 28, 2018
       https://doi.org/10.3892/ol.2018.9768
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Abstract

MicroRNAs (miRs) have been reported to serve key roles in cancer. To investigate the function of miR‑885‑5p in osteosarcoma, the expression levels of miR‑885‑5p were analyzed in 85 osteosarcoma tissue samples and adjacent non‑cancerous tissue samples, using reverse transcription‑quantitative polymerase chain reaction analysis. It was demonstrated that miR‑885‑5p was downregulated in osteosarcoma tissues and cell lines. Notably, the expression level of miR‑885‑5p was closely associated with tumor size, Tumor‑Node‑Metastasis stage and lymph node metastasis. Additionally, low expression levels of miR‑885‑5p also predicted a poor prognosis of osteosarcoma. To further decipher the roles of miR‑885‑5p in osteosarcoma, it was determined that β‑catenin, a key component of the Wnt signaling pathway, was a target of miR‑885‑5p. Furthermore, several functional experiments, including a colony formation assay, CCK‑8 assay, wound healing assay and Transwell invasion assay, revealed that miR‑885‑5p suppressed cell proliferation, migration and invasion through inhibition of β‑catenin. The results of the present study provide a novel insight into the molecular roles of miR‑885‑5p in osteosarcoma.
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1 

Ottaviani G and Jaffe N: The epidemiology of osteosarcoma. Cancer Treat Res. 152:3–13. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Luetke A, Meyers PA, Lewis I and Juergens H: Osteosarcoma treatment-where do we stand? A state of the art review. Cancer Treat Rev. 40:523–532. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Salah S, Ahmad R, Sultan I, Yaser S and Shehadeh A: Osteosarcoma with metastasis at initial diagnosis: Current outcomes and prognostic factors in the context of a comprehensive cancer center. Mol Clin Oncol. 2:811–816. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Subbiah V and Kurzrock R: Phase 1 clinical trials for sarcomas: The cutting edge. Curr Opin Oncol. 23:352–360. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Toiyama Y, Takahashi M, Hur K, Nagasaka T, Tanaka K, Inoue Y, Kusunoki M, Boland CR and Goel A: Serum miR-21 as a diagnostic and prognostic biomarker in colorectal cancer. J Natl Cancer Inst. 105:849–859. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Meng X, Wu J, Pan C, Wang H, Ying X, Zhou Y, Yu H, Zuo Y, Pan Z, Liu RY and Huang W: Genetic and epigenetic down-regulation of microRNA-212 promotes colorectal tumor metastasis via dysregulation of MnSOD. Gastroenterology. 145:426–436, e421-e426. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Hur K, Toiyama Y, Takahashi M, Balaguer F, Nagasaka T, Koike J, Hemmi H, Koi M, Boland CR and Goel A: MicroRNA-200c modulates epithelial-to-mesenchymal transition (EMT) in human colorectal cancer metastasis. Gut. 62:1315–1326. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Cekaite L, Rantala JK, Bruun J, Guriby M, Agesen TH, Danielsen SA, Lind GE, Nesbakken A, Kallioniemi O, Lothe RA and Skotheim RI: miR-9, −31, and −182 deregulation promote proliferation and tumor cell survival in colon cancer. Neoplasia. 14:868–879. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Schultz NA, Dehlendorff C, Jensen BV, Bjerregaard JK, Nielsen KR, Bojesen SE, Calatayud D, Nielsen SE, Yilmaz M, Holländer NH, et al: MicroRNA biomarkers in whole blood for detection of pancreatic cancer. JAMA. 311:392–404. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Xiang Y, Lu DL, Li JP, Yu CX, Zheng DL, Huang X, Wang ZY, Hu P, Liao XH and Zhang TC: Myocardin inhibits estrogen receptor alpha-mediated proliferation of human breast cancer MCF-7 cells via regulating MicroRNA expression. IUBMB Life. 68:477–487. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Zhang J, Wang D, Xiong J, Chen L and Huang J: MicroRNA-33a-5p suppresses growth of osteosarcoma cells and is downregulated in human osteosarcoma. Oncol Lett. 10:2135–2141. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Cai Y, Cai T and Chen Y: Wnt pathway in osteosarcoma, from oncogenic to therapeutic. J Cell Biochem. 115:625–631. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Mora-Blanco EL, Mishina Y, Tillman EJ, Cho YJ, Thom CS, Pomeroy SL, Shao W and Roberts CW: Activation of beta-catenin/TCF targets following loss of the tumor suppressor SNF5. Oncogene. 33:933–938. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Tetsu O and McCormick F: Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells. Nature. 398:422–426. 1999. View Article : Google Scholar : PubMed/NCBI

15 

Li YJ, Wei ZM, Meng YX and Ji XR: Beta-catenin up-regulates the expression of cyclinD1, c-myc and mmP-7 in human pancreatic cancer: Relationships with carcinogenesis and metastasis. World J Gastroenterol. 11:2117–2123. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Trierweiler C, Blum HE and Hasselblatt P: The transcription factor c-Jun protects against liver damage following activated beta-catenin signaling. PLoS One. 7:e406382012. View Article : Google Scholar : PubMed/NCBI

17 

Muroya S, Taniguchi M, Shibata M, Oe M, Ojima K, Nakajima I and Chikuni K: Profiling of differentially expressed microRNA and the bioinformatic target gene analyses in bovine fast- and slow-type muscles by massively parallel sequencing. J Anim Sci. 91:90–103. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Mao XH, Chen M, Wang Y, Cui PG, Liu SB and Xu ZY: MicroRNA-21 regulates the ERK/NF-kappaB signaling pathway to affect the proliferation, migration, and apoptosis of human melanoma A375 cells by targeting SPRY1, PDCD4, and PTEN. Mol Carcinog. 56:886–894. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Bing L, Hong C, Li-Xin S and Wei G: MicroRNA-543 suppresses endometrial cancer oncogenicity via targeting FAK and TWIST1 expression. Arch Gynecol Obstet. 290:533–541. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Wickstrom M, Dyberg C, Milosevic J, Einvik C, Calero R, Sveinbjörnsson B, Sandén E, Darabi A, Siesjö P, Kool M, et al: Wnt/beta-catenin pathway regulates mgMT gene expression in cancer and inhibition of Wnt signalling prevents chemoresistance. Nat Commun. 6:89042015. View Article : Google Scholar : PubMed/NCBI

22 

Hsieh IS, Chang KC, Tsai YT, Ke JY, Lu PJ, Lee KH, Yeh SD, Hong TM and Chen YL: MicroRNA-320 suppresses the stem cell-like characteristics of prostate cancer cells by downregulating the Wnt/beta-catenin signaling pathway. Carcinogenesis. 34:530–538. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Ma Y, Ren Y, Han EQ, Li H, Chen D, Jacobs JJ, Gitelis S, O'Keefe RJ, Konttinen YT, Yin G and Li TF: Inhibition of the Wnt-beta-catenin and Notch signaling pathways sensitizes osteosarcoma cells to chemotherapy. Biochem Biophys Res Commun. 431:274–279. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Afanasyeva EA, Mestdagh P, Kumps C, Vandesompele J, Ehemann V, Theissen J, Fischer M, Zapatka M, Brors B, Savelyeva L, et al: MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits proliferation and survival. Cell Death Differ. 18:974–984. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Lam CS, Ng L, Chow AK, Wan TM, Yau S, Cheng NS, Wong SK, Man JH, Lo OS, Foo DC, et al: Identification of microRNA 885-5p as a novel regulator of tumor metastasis by targeting CPEB2 in colorectal cancer. Oncotarget. 8:26858–26870. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Bao Z, Tian W and Huang G: miR‑885‑5p suppresses osteosarcoma proliferation, migration and invasion through regulation of β‑catenin. Oncol Lett 17: 1996-2004, 2019.
APA
Liu, Y., Bao, Z., Tian, W., & Huang, G. (2019). miR‑885‑5p suppresses osteosarcoma proliferation, migration and invasion through regulation of β‑catenin. Oncology Letters, 17, 1996-2004. https://doi.org/10.3892/ol.2018.9768
MLA
Liu, Y., Bao, Z., Tian, W., Huang, G."miR‑885‑5p suppresses osteosarcoma proliferation, migration and invasion through regulation of β‑catenin". Oncology Letters 17.2 (2019): 1996-2004.
Chicago
Liu, Y., Bao, Z., Tian, W., Huang, G."miR‑885‑5p suppresses osteosarcoma proliferation, migration and invasion through regulation of β‑catenin". Oncology Letters 17, no. 2 (2019): 1996-2004. https://doi.org/10.3892/ol.2018.9768
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Bao Z, Tian W and Huang G: miR‑885‑5p suppresses osteosarcoma proliferation, migration and invasion through regulation of β‑catenin. Oncol Lett 17: 1996-2004, 2019.
APA
Liu, Y., Bao, Z., Tian, W., & Huang, G. (2019). miR‑885‑5p suppresses osteosarcoma proliferation, migration and invasion through regulation of β‑catenin. Oncology Letters, 17, 1996-2004. https://doi.org/10.3892/ol.2018.9768
MLA
Liu, Y., Bao, Z., Tian, W., Huang, G."miR‑885‑5p suppresses osteosarcoma proliferation, migration and invasion through regulation of β‑catenin". Oncology Letters 17.2 (2019): 1996-2004.
Chicago
Liu, Y., Bao, Z., Tian, W., Huang, G."miR‑885‑5p suppresses osteosarcoma proliferation, migration and invasion through regulation of β‑catenin". Oncology Letters 17, no. 2 (2019): 1996-2004. https://doi.org/10.3892/ol.2018.9768
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