miR‑7‑5p inhibits cell migration and invasion in glioblastoma through targeting SATB1

Yin,Chang‑You; Kong,Wei; Jiang,Jing; Xu,Hao; Zhao,Wei

doi:10.3892/ol.2018.9777

miR‑7‑5p inhibits cell migration and invasion in glioblastoma through targeting SATB1

Authors:
- Chang‑You Yin
- Wei Kong
- Jing Jiang
- Hao Xu
- Wei Zhao
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Affiliations: Department of Neurosurgery, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China, Department of Emergency, Yantaishan Hospital, Yantai, Shandong 264001, P.R. China
Published online on: November 28, 2018 https://doi.org/10.3892/ol.2018.9777
Pages: 1819-1825

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Abstract

MicroRNAs (miRNAs/miRs) have been revealed to influence the development and progression of glioblastoma. Although a number of miRNAs are abnormally expressed in glioblastoma it is not clear whether they are a factor associated with glioblastoma pathogenesis. In the present study, miR‑7‑5p was identified as being aberrantly downregulated in glioblastoma tissues and cell lines. miR‑7‑5p overexpression significantly decreased the migratory and invasive capacity of the cells, while miR‑7‑5p silencing had the opposite effect. In addition, a luciferase assay confirmed that special AT rich sequence binding protein 1 (SATB1) was a direct target gene of miR‑7‑5p in glioblastoma. The overexpression of SATB1 in glioblastoma was revealed to promote cell migration and invasion. In addition, SATB1 overexpression may weaken the inhibitory effect of miR‑7‑5p on cell migration and invasion. miR‑7‑5p overexpression reversed the effects of SATB1 on cell migration and invasion in glioblastoma cells. In conclusion, miR‑7‑5p may be a useful therapeutic target for the diagnosis and treatment of patients with glioblastoma.

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1	Bradshaw A, Wickremsekera A, Tan ST, Peng L, Davis PF and Itinteang T: Cancer stem cell hierarchy in glioblastoma multiforme. Front Surg. 3:212016. View Article : Google Scholar : PubMed/NCBI
2	Khosla D: Concurrent therapy to enhance radiotherapeutic outcomes in glioblastoma. Ann Transl Med. 4:542016.PubMed/NCBI
3	Franceschi S, Mazzanti CM, Lessi F, Aretini P, Carbone FG, LA Ferla M, Scatena C, Ortenzi V, Vannozzi R, Fanelli G, et al: Investigating molecular alterations to profile short- and long-term recurrence-free survival in patients with primary glioblastoma. Oncol Lett. 10:3599–3606. 2015. View Article : Google Scholar : PubMed/NCBI
4	Tsai MM, Wang CS, Tsai CY, Huang HW, Chi HC, Lin YH, Lu PH and Lin KH: Potential diagnostic, prognostic and therapeutic targets of microRNAs in human gastric cancer. Int J Mol Sci. 17(pii): E9452016. View Article : Google Scholar : PubMed/NCBI
5	Dallaire A and Simard MJ: The implication of microRNAs and endo-siRNAs in animal germline and early development. Dev Biol. 416:18–25. 2016. View Article : Google Scholar : PubMed/NCBI
6	Cimmino A, Calin GA, Fabbri M, Iorio MV, Ferracin M, Shimizu M, Wojcik SE, Aqeilan RI, Zupo S, Dono M, et al: miR-15 and miR-16 induce apoptosis by targeting BCL2. Proc Natl Acad Sci USA. 102:13944–13949. 2005. View Article : Google Scholar : PubMed/NCBI
7	Asangani IA, Rasheed SA, Nikolova DA, Leupold JH, Colburn NH, Post S and Allgayer H: MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer. Oncogene. 27:2128–2136. 2008. View Article : Google Scholar : PubMed/NCBI
8	Wei S, Zhang ZY, Fu SL, Xie JG, Liu XS, Xu YJ, Zhao JP and Xiong WN: Hsa-miR-623 suppresses tumor progression in human lung adenocarcinoma. Cell Death Dis. 8:e28292017. View Article : Google Scholar : PubMed/NCBI
9	Gui ZL, Wu TL, Zhao GC, Lin ZX and Xu HG: MicroRNA-497 suppress osteosarcoma by targeting MAPK/Erk pathway. Bratisl Lek Listy. 118:449–452. 2017.PubMed/NCBI
10	Wu RS, Qiu EH, Zhu JJ, Wang JR and Lin HL: MiR-101 promotes nasopharyngeal carcinoma cell apoptosis through inhibiting Ras/Raf/MEK/ERK signaling pathway. Eur Rev Med Pharmacol Sci. 22:150–157. 2018.PubMed/NCBI
11	Zhang Z, Song X, Tian H, Miao Y, Feng X, Li Y and Wang H: MicroRNA-137 inhibits growth of glioblastoma through EGFR suppression. Am J Transl Res. 9:1492–1499. 2017.PubMed/NCBI
12	Hu S, Chen H, Zhang Y, Wang C, Liu K, Wang H and Luo J: MicroRNA-520c inhibits glioma cell migration and invasion by the suppression of transforming growth factor-β receptor type 2. Oncol Rep. 37:1691–1697. 2017. View Article : Google Scholar : PubMed/NCBI
13	Hao Y, Zhang S, Sun S, Zhu J and Xiao Y: miR-595 targeting regulation of SOX7 expression promoted cell proliferation of human glioblastoma. Biomed Pharmacother. 80:121–126. 2016. View Article : Google Scholar : PubMed/NCBI
14	Babae N, Bourajjaj M, Liu Y, Van Beijnum JR, Cerisoli F, Scaria PV, Verheul M, Van Berkel MP, Pieters EH, Van Haastert RJ, et al: Systemic miRNA-7 delivery inhibits tumor angiogenesis and growth in murine xenograft glioblastoma. Oncotarget. 5:6687–6700. 2014. View Article : Google Scholar : PubMed/NCBI
15	Zhang X, Zhang X, Hu S, Zheng M, Zhang J, Zhao J, Zhang X, Yan B, Jia L, Zhao J, et al: Identification of miRNA-7 by genome- wide analysis as a critical sensitizer for TRAIL-induced apoptosis in glioblastoma cells. Nucleic Acids Res. 45:5930–5944. 2017. View Article : Google Scholar : PubMed/NCBI
16	Zeng CY, Zhan YS, Huang J and Chen YX: MicroRNA-7 suppresses human colon cancer invasion and proliferation by targeting the expression of focal adhesion kinase. Mol Med Rep. 13:1297–1303. 2016. View Article : Google Scholar : PubMed/NCBI
17	Bi Y, Shen W, Min M and Liu Y: MicroRNA-7 functions as a tumor-suppressor gene by regulating ILF2 in pancreatic carcinoma. Int J Mol Med. 39:900–906. 2017. View Article : Google Scholar : PubMed/NCBI
18	Cao Q, Mao ZD, Shi YJ, Chen Y, Sun Y, Zhang Q, Song L and Peng LP: MicroRNA-7 inhibits cell proliferation, migration and invasion in human non-small cell lung cancer cells by targeting FAK through ERK/MAPK signaling pathway. Oncotarget. 7:77468–77481. 2016. View Article : Google Scholar : PubMed/NCBI
19	Hua K, Jin J, Zhang H, Zhao B, Wu C, Xu H and Fang L: MicroRNA-7 inhibits proliferation, migration and invasion of thyroid papillary cancer cells via targeting CKS2. Int J Oncol. 49:1531–1540. 2016. View Article : Google Scholar : PubMed/NCBI
20	Grens K: Popular tumor cell line contaminated. The Scientist, Midland, Ontario, 2016. https://www.the-scientist.com/?articles.view/articleNo/46929/title/Popular-Tumor-Cell-Line-Contaminated/Updated. August 31–2016
21	Gasteiger E: ExPASy Bioinformatics Resource Portal. 2003, https://web.expasy.org/cellosaurus/CVCL_2219
22	Allen M, Bjerke M, Edlund H, Nelander S and Westermark B: Origin of the U87MG glioma cell line: Good news and bad news. Sci Transl Med. 8:354re32016. View Article : Google Scholar : PubMed/NCBI
23	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
24	Short SC: Survival from brain tumors in England and Wales up to 2001. Br J Cancer. 99 Suppl 1:S102–S103. 2008. View Article : Google Scholar : PubMed/NCBI
25	Kefas B, Godlewski J, Comeau L, Li Y, Abounader R, Hawkinson M, Lee J, Fine H, Chiocca EA, Lawler S and Purow B: MicroRNA-7 inhibits the epidermal growth factor receptor and the AKT pathway and is down-regulated in glioblastoma. Cancer Res. 68:3566–3572. 2008. View Article : Google Scholar : PubMed/NCBI
26	Liu Z, Jiang Z, Huang J, Huang S, Li Y, Yu S, Yu S and Liu X: miR-7 inhibits glioblastoma growth by simultaneously interfering with the PI3K/ATK and RAF/MEK/ERK pathways. Int J Oncol. 44:1571–1580. 2014. View Article : Google Scholar : PubMed/NCBI
27	Liu Z, Liu Y, Li L, Xu Z, Bi B, Wang Y and Li JY: miR-7-5p is frequently downregulated in glioblastoma microvasculature and inhibits vascular endothelial cell proliferation by targeting RAF1. Tumor Biol. 35:10177–10184. 2014. View Article : Google Scholar
28	Wang J, Liu H, Tian L, Wang F, Han L, Zhang W and Bai YA: miR-15b inhibits the progression of glioblastoma cells through targeting insulin-like growth factor receptor 1. Horm Cancer. 8:49–57. 2017. View Article : Google Scholar : PubMed/NCBI
29	Wang X, Sun S, Tong X, Ma Q, Di H, Fu T, Sun Z, Cai Y, Fan W, Wu Q, et al: miRNA-154-5p inhibits cell proliferation and metastasis by targeting PIWIL1 in glioblastoma. Brain Res. 1676:69–76. 2017. View Article : Google Scholar : PubMed/NCBI
30	Song S, Fajol A, Tu X, Ren B and Shi S: miR-204 suppresses the development and progression of human glioblastoma by targeting ATF2. Oncotarget. 7:70058–70065. 2016. View Article : Google Scholar : PubMed/NCBI
31	Lv C, Bai Z, Liu Z, Luo P and Zhang J: MicroRNA-495 suppresses human renal cell carcinoma malignancy by targeting SATB1. Am J Transl Res. 7:1992–1999. 2015.PubMed/NCBI
32	Wang G, Li B, Fu Y, He M, Wang J, Shen P and Bai L: miR-23a suppresses proliferation of osteosarcoma cells by targeting SATB1. Tumour Biol. 36:4715–4721. 2015. View Article : Google Scholar : PubMed/NCBI
33	Han B, Luan L, Xu Z and Wu B: Expression and biological roles of SATB1 in human bladder cancer. Tumor Biol. 34:2943–2949. 2013. View Article : Google Scholar
34	Han S, Xia J, Qin X, Han S and Wu A: Phosphorylated SATB1 is associated with the progression and prognosis of glioma. Cell Death Dis. 4:e9012013. View Article : Google Scholar : PubMed/NCBI
35	Chu SH, Ma YB, Feng DF, Zhang H, Zhu ZA, Li ZQ and Jiang PC: Upregulation of SATB1 is associated with the development and progression of glioma. J Transl Med. 10:1492012. View Article : Google Scholar : PubMed/NCBI
36	Mcinnes N, Sadlon TJ, Brown CY, Pederson S, Beyer M, Schultze JL, McColl S, Goodall GJ and Barry SC: FOXP3 and FOXP3-regulated microRNAs suppress SATB1 in breast cancer cells. Oncogene. 31:1045–1054. 2012. View Article : Google Scholar : PubMed/NCBI