Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
February-2019 Volume 17 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2019 Volume 17 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Ophiopogonin D inhibits cell proliferation and induces apoptosis of human laryngocarcinoma through downregulation of cyclin B1 and MMP‑9 and upregulation of p38‑MAPK signaling

  • Authors:
    • Zhiyu Yan
    • Guang Liu
    • Min Liang
    • Yanjun Xu
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology, Beijing Military General Hospital, Beijing 100700, P.R. China, Department of Pathology, Beijing Military General Hospital, Beijing 100700, P.R. China
  • Pages: 1877-1882
    |
    Published online on: December 3, 2018
       https://doi.org/10.3892/ol.2018.9788
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The pharmacological actions of Ophiopogonin D include resistance to cardiovascular and cerebrovascular diseases, anti‑aging effects, improvement in learning deficit and dysmnesia, anti‑tumor, anti‑radiation and anti‑inflammatory effects, immunoregulation, and the relief of cough and hepatopulmonary pathological lesions. However, the efficacy of Ophiopogonin D on human laryngocarcinoma remains to be elucidated. The present study therefore investigated whether the anti‑cancer effect of Ophiopogonin D inhibits cell proliferation and induces apoptosis of human laryngocarcinoma. In the present study, it was found that Ophiopogonin D inhibited cell proliferation, promoted cytotoxicity, induced apoptosis and increased caspase‑3/9 activity in human laryngocarcinoma cells. Ophiopogonin D significantly suppressed cyclin B1 and matrix metalloproteinase‑9 (MMP‑9) protein expression, and upregulated p‑p38 MAPK protein expression in human laryngocarcinoma cells. Together, these results suggest Ophiopogonin D inhibits cell proliferation and induced apoptosis in human laryngocarcinoma cells through downregulation of cyclin B1 and MMP‑9 and upregulation of the p38 MAPK signaling pathway. Therefore, Ophiopogonin D may be a potential therapy for the treatment of human laryngocarcinoma.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Dong QL and Wang GN: Effect of general anaesthesia with combination of acupuncture and enflurane applied in radical operation of laryngocarcinoma. Chin J Integr Med. 12:306–309. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Chen LW, Wang JL, Zhang LY, Yang SM, Li CS, Yu N, Zhao W JD, Zhao LD, Li K, Liu MB and Zhai SQ: Establishment of an animal model of spontaneous cervical lymph node metastasis of laryngeal squamous cell carcinoma and obtaining laryngocarcinoma cells with high metastatic potential. Neoplasma. 60:504–510. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Jiang LY, Lian M, Wang H, Fang JG and Wang Q: Inhibitory effects of 5-Aza-2′-deoxycytidine and trichostatin A in combination with p53-expressing adenovirus on human laryngocarcinoma cells. Chin J Cancer Res. 24:232–237. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Wang XX, Yao XB, Qiang ZS and Zhu HL: Attenuation of EGFL7 inhibits human laryngocarcinoma cells growth and invasion. Int J Clin Exp Med. 8:3141–3155. 2015.PubMed/NCBI

5 

Fodor T, Szántó M, Abdul-Rahman O, Nagy L, Dér Á, Kiss B and Bai P: Combined treatment of MCF-7 cells with AICAR and methotrexate, arrests cell cycle and reverses warburg metabolism through AMP-activated protein kinase (AMPK) and FOXO1. PLoS One. 11:e01502322016. View Article : Google Scholar : PubMed/NCBI

6 

Makarević J, Tsaur I, Juengel E, Borgmann H, Nelson K, Thomas C, Bartsch G, Haferkamp A and Blaheta RA: Amygdalin delays cell cycle progression and blocks growth of prostate cancer cells in vitro. Life Sci. 147:137–142. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Liu YF, Qu GQ, Lu YM, Kong WM, Liu Y, Chen WX and Liao XH: Silencing of MAP4K4 by short hairpin RNA suppresses proliferation, induces G1 cell cycle arrest and induces apoptosis in gastric cancer cells. Mol Med Rep. 13:41–48. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Liu G, Ren X, Gao C and Zhang W: Acylglycerol kinase promotes the proliferation and cell cycle progression of oral squamous cell carcinoma. Mol Med Rep. 12:2225–2230. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Latocha M, Płonka J, Kuśmierz D, Jurzak M, Polaniak R and Nowosad A: Transcripional activity of genes encoding MMPs and TIMPs in breast cancer cells treated by genistein and in normal cancer-associated fibroblasts-in vitro studies. Acta Pol Pharm. 71:1095–1102. 2014.PubMed/NCBI

10 

Gurgel DC, Valença-Junior JT, Dornelas CA, Vieira RB, Maia-Filho JT, Lima-Junior RC, Ribeiro RA and Almeida PR: Immunoexpression of metalloproteinases 2 and 14 and TIMP-2 inhibitor in main types of primary gastric carcinomas and lymph node metastasis. Pathol Oncol Res. 21:73–81. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Navarini NF, Araújo VC, Brown AL, Passador-Santos F, Souza IF, Napimoga MH, Araújo NS and Martinez EF: The EGF signaling pathway influences cell migration and the secretion of metalloproteinases by myoepithelial cells in pleomorphic adenoma. Tumour Biol. 36:205–211. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Kang N, Hu G, Wu W, Jiang J and Yang X: The impact of PDTC on circulating tumor cells of laryngocarcinoma in nude mice model. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 25:419–422. 2011.(In Chinese). PubMed/NCBI

13 

Gaundar SS and Bendall LJ: The potential and limitations of p38MAPK as a drug target for the treatment of hematological malignancies. Curr Drug Targets. 11:823–833. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Noel JK, Crean S, Claflin JE, Ranganathan G, Linz H and Lahn M: Systematic review to establish the safety profiles for direct and indirect inhibitors of p38 Mitogen-activated protein kinases for treatment of cancer. A systematic review of the literature. Med Oncol. 25:323–330. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Zhang ZD, Li Y, Fan Q, Zhao B, Tan B and Zhao XF: Annexin A2 is implicated in multi-drug-resistance in gastric cancer through p38MAPK and AKT pathway. Neoplasma. 61:627–637. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Park SH, Lee HJ, Ryu J, Son KH, Kwon SY, Lee SK, Kim YS, Hong JH, Seok JH and Lee CJ: Effects of ophiopogonin D and spicatoside A derived from Liriope Tuber on secretion and production of mucin from airway epithelial cells. Phytomedicine. 21:172–176. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Ishibashi H, Mochidome T, Okai J, Ichiki H, Shimada H and Takahama K: Activation of potassium conductance by ophiopogonin-D in acutely dissociated rat paratracheal neurones. Br J Pharmacol. 132:461–466. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Zang QQ, Zhang L, Gao N and Huang C: Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G2/M, and induces apoptosis in human breast carcinoma MCF-7 cells. J Integr Med. 14:51–59. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Qian J, Jiang F, Wang B, Yu Y, Zhang X and Yin Z: Ophiopogonin D prevents H2O2-induced injury in primary human umbilical vein endothelial cells. J Ethnopharmacol. 128:438–445. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Li X, Shen Y, Di B, Li J, Geng J, Lu X and He Z: Biological and clinical significance of p75NTR expression in laryngeal squamous epithelia and laryngocarcinoma. Acta Otolaryngol. 132:314–324. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Lohberger B, Leithner A, Stuendl N, Kaltenegger H, Kullich W and Steinecker-Frohnwieser B: Diacerein retards cell growth of chondrosarcoma cells at the G2/M cell cycle checkpoint via cyclin B1/CDK1 and CDK2 downregulation. BMC Cancer. 15:8912015. View Article : Google Scholar : PubMed/NCBI

22 

Song Y, Zhao C, Dong L, Fu M, Xue L, Huang Z, Tong T, Zhou Z, Chen A, Yang Z, et al: Overexpression of cyclin B1 in human esophageal squamous cell carcinoma cells induces tumor cell invasive growth and metastasis. Carcinogenesis. 29:307–315. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Dao T, Korontsvit T, Zakhaleva V, Haro K, Packin J and Scheinberg DA: Identification of a human cyclin D1-derived peptide that induces human cytotoxic CD4 T cells. PLoS One. 4:e67302009. View Article : Google Scholar : PubMed/NCBI

24 

Luukkaa H, Klemi P, Leivo I, Mäkitie AA, Irish J, Gilbert R, Perez-Ordonez B, Hirsimäki P, Vahlberg T, Kivisaari A, et al: Expression of matrix metalloproteinase-1, −7, −9, −13, Ki-67, and HER-2 in epithelial-myoepithelial salivary gland cancer. Head Neck. 32:1019–1027. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Munaut C, Noël A, Hougrand O, Foidart JM, Boniver J and Deprez M: Vascular endothelial growth factor expression correlates with matrix metalloproteinases MT1-MMP, MMP-2 and MMP-9 in human glioblastomas. Int J Cancer. 106:848–855. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Zhang Y, Han Y, Zhai K, Sun M, Liu J, Yu B and Kou J: Ophiopogonin-D suppresses MDA-MB-435 cell adhesion and invasion by inhibiting matrix metalloproteinase-9. Mol Med Rep. 12:1493–1498. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Singh P, Sarkar S, Umar S, Rengifo-Cam W, Singh AP and Wood TG: Insulin-like growth factors are more effective than progastrin in reversing proapoptotic effects of curcumin: Critical role of p38MAPK. Am J Physiol Gastrointest Liver Physiol. 298:G551–G562. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Estrella VC, Eder AM, Liu S, Pustilnik TB, Tabassam FH, Claret FX, Gallick GE, Mills GB and Wiener JR: Lysophosphatidic acid induction of urokinase plasminogen activator secretion requires activation of the p38MAPK pathway. Int J Oncol. 31:441–449. 2007.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yan Z, Liu G, Liang M and Xu Y: Ophiopogonin D inhibits cell proliferation and induces apoptosis of human laryngocarcinoma through downregulation of cyclin B1 and MMP‑9 and upregulation of p38‑MAPK signaling. Oncol Lett 17: 1877-1882, 2019.
APA
Yan, Z., Liu, G., Liang, M., & Xu, Y. (2019). Ophiopogonin D inhibits cell proliferation and induces apoptosis of human laryngocarcinoma through downregulation of cyclin B1 and MMP‑9 and upregulation of p38‑MAPK signaling. Oncology Letters, 17, 1877-1882. https://doi.org/10.3892/ol.2018.9788
MLA
Yan, Z., Liu, G., Liang, M., Xu, Y."Ophiopogonin D inhibits cell proliferation and induces apoptosis of human laryngocarcinoma through downregulation of cyclin B1 and MMP‑9 and upregulation of p38‑MAPK signaling". Oncology Letters 17.2 (2019): 1877-1882.
Chicago
Yan, Z., Liu, G., Liang, M., Xu, Y."Ophiopogonin D inhibits cell proliferation and induces apoptosis of human laryngocarcinoma through downregulation of cyclin B1 and MMP‑9 and upregulation of p38‑MAPK signaling". Oncology Letters 17, no. 2 (2019): 1877-1882. https://doi.org/10.3892/ol.2018.9788
Copy and paste a formatted citation
x
Spandidos Publications style
Yan Z, Liu G, Liang M and Xu Y: Ophiopogonin D inhibits cell proliferation and induces apoptosis of human laryngocarcinoma through downregulation of cyclin B1 and MMP‑9 and upregulation of p38‑MAPK signaling. Oncol Lett 17: 1877-1882, 2019.
APA
Yan, Z., Liu, G., Liang, M., & Xu, Y. (2019). Ophiopogonin D inhibits cell proliferation and induces apoptosis of human laryngocarcinoma through downregulation of cyclin B1 and MMP‑9 and upregulation of p38‑MAPK signaling. Oncology Letters, 17, 1877-1882. https://doi.org/10.3892/ol.2018.9788
MLA
Yan, Z., Liu, G., Liang, M., Xu, Y."Ophiopogonin D inhibits cell proliferation and induces apoptosis of human laryngocarcinoma through downregulation of cyclin B1 and MMP‑9 and upregulation of p38‑MAPK signaling". Oncology Letters 17.2 (2019): 1877-1882.
Chicago
Yan, Z., Liu, G., Liang, M., Xu, Y."Ophiopogonin D inhibits cell proliferation and induces apoptosis of human laryngocarcinoma through downregulation of cyclin B1 and MMP‑9 and upregulation of p38‑MAPK signaling". Oncology Letters 17, no. 2 (2019): 1877-1882. https://doi.org/10.3892/ol.2018.9788
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team