Open Access

Apatinib enhances the radiosensitivity of the esophageal cancer cell line KYSE‑150 by inducing apoptosis and cell cycle redistribution

  • Authors:
    • Lijun Hu
    • Fei Sun
    • Zhiqiang Sun
    • Xinchu Ni
    • Jian Wang
    • Jianlin Wang
    • Mengyun Zhou
    • Yue Feng
    • Ze Kong
    • Qiu Hua
    • Jingping Yu
  • View Affiliations

  • Published online on: December 6, 2018     https://doi.org/10.3892/ol.2018.9803
  • Pages: 1609-1616
  • Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

To determine the radiosensitizing effect of apatinib on esophageal cancer cells, and to preliminarily investigate the underlying mechanism, KYSE‑150 cells were treated with apatinib, x‑ray or apatinib combined with x‑ray, and compared with a blank control. It was observed that apatinib significantly inhibited vascular endothelial growth factor (VEGF) secretion and the proliferation of KYSE‑150 cells in a dose‑dependent manner. As the concentration of apatinib increased, the radiobiological parameters inactivation dose (D0), quasi domain does (Dq) and survival fraction (SF2) of KYSE‑150 cells decreased, while the sensitization enhancement ratio SERD0 increased. The rate of apoptosis in cells treated with apatinib and x‑ray was markedly higher compared with those of the blank control, x‑ray and apatinib alone groups (P<0.05). The proportion of cells in the G2/M phase was significantly increased in the apatinib, x‑ray and combination groups compared with the blank control group (P<0.05). Compared with the control and x‑ray groups, combination treatment did not significantly alter the expression level of polyADP‑ribose polymerase (PARP), although it significantly increased the expression of cleaved‑PARP (P<0.05). Moreover, the expression of cell serine/threonine‑protein kinase‑2 (CHK2) was downregulated (P<0.05), whilst expression of the phosphorylated form, pCHK2, was significantly increased (P<0.05) in the combination group when compared with the control and x‑ray groups. In conclusion, the present study suggested that apatinib increases the radiosensitivity of KYSE‑150 esophageal cancer cells by inhibiting VEGF secretion and cell proliferation, and promoting apoptosis and cell cycle redistribution.
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February-2019
Volume 17 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Hu L, Sun F, Sun Z, Ni X, Wang J, Wang J, Zhou M, Feng Y, Kong Z, Hua Q, Hua Q, et al: Apatinib enhances the radiosensitivity of the esophageal cancer cell line KYSE‑150 by inducing apoptosis and cell cycle redistribution. Oncol Lett 17: 1609-1616, 2019
APA
Hu, L., Sun, F., Sun, Z., Ni, X., Wang, J., Wang, J. ... Yu, J. (2019). Apatinib enhances the radiosensitivity of the esophageal cancer cell line KYSE‑150 by inducing apoptosis and cell cycle redistribution. Oncology Letters, 17, 1609-1616. https://doi.org/10.3892/ol.2018.9803
MLA
Hu, L., Sun, F., Sun, Z., Ni, X., Wang, J., Wang, J., Zhou, M., Feng, Y., Kong, Z., Hua, Q., Yu, J."Apatinib enhances the radiosensitivity of the esophageal cancer cell line KYSE‑150 by inducing apoptosis and cell cycle redistribution". Oncology Letters 17.2 (2019): 1609-1616.
Chicago
Hu, L., Sun, F., Sun, Z., Ni, X., Wang, J., Wang, J., Zhou, M., Feng, Y., Kong, Z., Hua, Q., Yu, J."Apatinib enhances the radiosensitivity of the esophageal cancer cell line KYSE‑150 by inducing apoptosis and cell cycle redistribution". Oncology Letters 17, no. 2 (2019): 1609-1616. https://doi.org/10.3892/ol.2018.9803