Open Access

Correlation of ERK/MAPK signaling pathway with proliferation and apoptosis of colon cancer cells

  • Authors:
    • Gang Zhou
    • Jing Yang
    • Peng Song
  • View Affiliations

  • Published online on: December 20, 2018     https://doi.org/10.3892/ol.2018.9857
  • Pages: 2266-2270
  • Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The role of extracellular signal‑regulated kinase/mito­gen‑activated protein kinase (ERK/MAPK) signaling pathway in the proliferation and apoptosis of human colon cancer cells was studied. The transduction process of ERK/MAPK signaling pathway was inhibited using methyl ethyl ketone (MEK) inhibitor U0126. Promoting effect of hepatocyte growth factor (HGF) on proliferation of human colon cancer cells was detected via Cell Counting Kit 8 (CCK8), the cycle and apoptosis of human colon cancer cells were detected via flow cytometry, and the migration of human colon cancer cells was detected via wound healing assay. The results revealed that after drug treatment for 48 h, there were statistically significant differences in 4 and 8 µmol/l U0126 experimental group compared with control group (P<0.05). Compared with those in control group, G1 phase, S phase, G2 phase and proliferation index (PI) in 2, 4 and 8 µmol/l U0126 group had statistically significant differences (P<0.05). There were statistically significant differences in comparison of G1 phase, S phase, G2 phase and PI between control and 8 µmol/l U0126 group (P<0.05). Compared with that in control group, the cell migration distance in 8 µmol/l U0126 group had a statistically significant difference after drug treatment for 24 h (P<0.05). After drug treatment for 48 and 72 h, the cell migration distance in 4 and 8 µmol/l U0126 group was significantly reduced, and the differences were statistically significant compared with that in control group (P<0.05). In conclusion, ERK/MAPK signaling pathway is involved in the effects of HGF of promoting proliferation and regulating cell cycle and apoptosis of human colon cancer cells, providing a new approach for the treatment of colon cancer.
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February-2019
Volume 17 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhou G, Yang J and Song P: Correlation of ERK/MAPK signaling pathway with proliferation and apoptosis of colon cancer cells. Oncol Lett 17: 2266-2270, 2019
APA
Zhou, G., Yang, J., & Song, P. (2019). Correlation of ERK/MAPK signaling pathway with proliferation and apoptosis of colon cancer cells. Oncology Letters, 17, 2266-2270. https://doi.org/10.3892/ol.2018.9857
MLA
Zhou, G., Yang, J., Song, P."Correlation of ERK/MAPK signaling pathway with proliferation and apoptosis of colon cancer cells". Oncology Letters 17.2 (2019): 2266-2270.
Chicago
Zhou, G., Yang, J., Song, P."Correlation of ERK/MAPK signaling pathway with proliferation and apoptosis of colon cancer cells". Oncology Letters 17, no. 2 (2019): 2266-2270. https://doi.org/10.3892/ol.2018.9857