Expression of miR‑21 and miR‑138 in colon cancer and its effect on cell proliferation and prognosis

You,Changxuan; Jin,Liming; Xu,Qi; Shen,Bo; Jiao,Xuelong; Huang,Xuewu

doi:10.3892/ol.2018.9864

Expression of miR‑21 and miR‑138 in colon cancer and its effect on cell proliferation and prognosis

Authors:
- Changxuan You
- Liming Jin
- Qi Xu
- Bo Shen
- Xuelong Jiao
- Xuewu Huang
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Affiliations: Department of Medical Oncology, Nanfang Hospital, Guangzhou, Guangdong 510515, P.R. China, Department of General Surgery, The Affiliated People's Hospital, Hangzhou College, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China, Department of Abdominal Μedical Οncology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China, Department of Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, Jiangsu 210009, P.R. China, Department of General Surgery, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China, Department of Oncology Center, First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China
Published online on: December 21, 2018 https://doi.org/10.3892/ol.2018.9864
Pages: 2271-2277
Copyright: © You et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Effect of miR‑21 and miR‑138 on the proliferation of colon cancer cells and their association with prognosis were investigated. Expression levels of miR‑21 and miR‑138 in colorectal cancer and normal adjacent tissues were compared. Differential expression of miR‑21 and miR‑138 in colon cancer tissues with different clinicopathological features were analyzed. miR‑21 and miR‑138 expression vectors were established and transfected into cells of colon cancer cell line SW480. Methyl thiazolyl tetrazolium (MTT) assay was used to detect the proliferation of SW480 cells. Kaplan‑Meier method and log‑rank test were used to study the relationship between miR‑21 and miR‑138 expression and prognosis. Cox proportional hazards model was used to analyze the factors related to prognosis of colon cancer. Expression level of miR‑21 in colon cancer tissues was significantly higher than that in adjacent tissues, and expression level of miR‑138 was lower in cancer tissues than in adjacent tissues (P<0.001). Expression of miR‑21 and miR‑138 was associated with the degree of differentiation, lymph node metastasis, distant metastasis, and TNM stage (P<0.05). miR‑21 promotes proliferation of colon cancer cell line SW480, and miR‑138 inhibits cell proliferation. Survival analysis showed that the survival time of patients with high expression of miR‑21 was significantly shorter than that of patients with low expression of miR‑21, while survival time of patients with high expression of miR‑138 was significantly longer than that of patients with low expression of miR‑138 (log‑rank, P<0.05). miR‑21 is highly expressed in colon cancer tissues and is positively associated with the degree of malignancy of patients but negatively associated with survival. miR‑138 expression is low in colon cancer tissues and is negatively associated with the degree of malignancy of patients but positively associated with survival. miR‑21 and miR‑138 may be involved in the regulation of colon cancer cell proliferation.

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