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Article Open Access

Isolation of circulating tumor cells in patients undergoing surgery for esophageal cancer and a specific confirmation method.

Corrigendum in: /10.3892/ol.2019.10878
  • Authors:
    • Yunpeng Zhao
    • Shukang Zhao
    • Yingjie Chen
    • Xiaopeng Dong
    • Chuanliang Peng
    • Qifeng Sun
    • Lei Shan
    • Zhendan Wang
    • Xiaogang Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Department of Thoracic Surgery, Shandong Cancer Hospital and Institute (HL), Jinan, Shandong 250177, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3817-3825
    |
    Published online on: February 6, 2019
       https://doi.org/10.3892/ol.2019.10017
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Abstract

The clinical significance of circulating tumor cells (CTCs) in patients with esophageal squamous cell carcinoma (ESCC) who have undergone radical surgery was investigated. A novel confirmation method for identifying CTCs or circulating tumor microemboli (CTM) in ESCC was also investigated. Blood samples from 55 patients with ESCC were collected 1‑3 days prior to surgery and 7 days post‑surgery. All patients underwent curative thoracic esophagectomy and lymphadenectomy. Blood samples from 20 healthy volunteers were obtained as controls. Isolation by size of epithelial tumor cells (ISET) was performed also. The overall CTC detection rate was 52.7% preoperatively and 49.1% postoperatively. The presence of CTCs correlated with the Tumor‑Node‑Metastasis stage and the Log odds of positive lymph nodes. No significant difference in perioperative CTC transformation was discovered between the thoracoscopic and laparoscopic approach, and the open approach. The P40+/cluster of differentiation (CD)45‑ phenotype was confirmed in the CTCs and CTM. ISET appeared to have high sensitivity for detecting CTCs within ESCC patients. Immunofluorescence staining for CD45 and P40 was a specific, accurate and convenient method for confirming the presence of CTCs or CTM in patients with ESCC, and is strongly recommended as a supplement to morphological analysis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao Y, Zhao S, Chen Y, Dong X, Peng C, Sun Q, Shan L, Wang Z and Zhao X: Isolation of circulating tumor cells in patients undergoing surgery for esophageal cancer and a specific confirmation method. Corrigendum in /10.3892/ol.2019.10878. Oncol Lett 17: 3817-3825, 2019.
APA
Zhao, Y., Zhao, S., Chen, Y., Dong, X., Peng, C., Sun, Q. ... Zhao, X. (2019). Isolation of circulating tumor cells in patients undergoing surgery for esophageal cancer and a specific confirmation method. Corrigendum in /10.3892/ol.2019.10878. Oncology Letters, 17, 3817-3825. https://doi.org/10.3892/ol.2019.10017
MLA
Zhao, Y., Zhao, S., Chen, Y., Dong, X., Peng, C., Sun, Q., Shan, L., Wang, Z., Zhao, X."Isolation of circulating tumor cells in patients undergoing surgery for esophageal cancer and a specific confirmation method. Corrigendum in /10.3892/ol.2019.10878". Oncology Letters 17.4 (2019): 3817-3825.
Chicago
Zhao, Y., Zhao, S., Chen, Y., Dong, X., Peng, C., Sun, Q., Shan, L., Wang, Z., Zhao, X."Isolation of circulating tumor cells in patients undergoing surgery for esophageal cancer and a specific confirmation method. Corrigendum in /10.3892/ol.2019.10878". Oncology Letters 17, no. 4 (2019): 3817-3825. https://doi.org/10.3892/ol.2019.10017
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Y, Zhao S, Chen Y, Dong X, Peng C, Sun Q, Shan L, Wang Z and Zhao X: Isolation of circulating tumor cells in patients undergoing surgery for esophageal cancer and a specific confirmation method. Corrigendum in /10.3892/ol.2019.10878. Oncol Lett 17: 3817-3825, 2019.
APA
Zhao, Y., Zhao, S., Chen, Y., Dong, X., Peng, C., Sun, Q. ... Zhao, X. (2019). Isolation of circulating tumor cells in patients undergoing surgery for esophageal cancer and a specific confirmation method. Corrigendum in /10.3892/ol.2019.10878. Oncology Letters, 17, 3817-3825. https://doi.org/10.3892/ol.2019.10017
MLA
Zhao, Y., Zhao, S., Chen, Y., Dong, X., Peng, C., Sun, Q., Shan, L., Wang, Z., Zhao, X."Isolation of circulating tumor cells in patients undergoing surgery for esophageal cancer and a specific confirmation method. Corrigendum in /10.3892/ol.2019.10878". Oncology Letters 17.4 (2019): 3817-3825.
Chicago
Zhao, Y., Zhao, S., Chen, Y., Dong, X., Peng, C., Sun, Q., Shan, L., Wang, Z., Zhao, X."Isolation of circulating tumor cells in patients undergoing surgery for esophageal cancer and a specific confirmation method. Corrigendum in /10.3892/ol.2019.10878". Oncology Letters 17, no. 4 (2019): 3817-3825. https://doi.org/10.3892/ol.2019.10017
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