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Gene expression profile analysis of ENO1 knockdown in gastric cancer cell line MGC‑803

  • Authors:
    • Zhigang Huang
    • Bode Lin
    • Haiyan Pan
    • Jinlin Du
    • Rongwei He
    • Shizhuo Zhang
    • Ping Ouyang
  • View Affiliations / Copyright

    Affiliations: Department of Epidemiology and Health Statistics, School of Public Health, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3881-3889
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    Published online on: February 19, 2019
       https://doi.org/10.3892/ol.2019.10053
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Abstract

Gastric cancer (GC) is the third leading cause of cancer‑associated mortality. In a previous study, we identified that α‑enolase (ENO1) promoted cell migration in GC, but the underlying molecular mechanisms remain to be fully elucidated. In the present study, small interfering RNAs were identified to interfere with ENO1 expression. The cDNA expression profiling was performed using an Affymetrix mRNA array platform to identify genes that may be associated with ENO1 in human GC cell line MGC‑803. The differentially expressed genes (DEGs) were identified using the reverse transcription‑quantitative polymerase chain reaction, followed by a series of bioinformatic analyses. As a result, there were 448 DEGs, among which 183 (40.85%) were downregulated. The most significant functional terms for the DEGs were the nuclear lumen for cell components (P=2.83x10‑4), transcription for biological processes (P=3.7x10‑7) and transcription factor activity for molecular functions (P=1.16x104). In total, six significant pathways were enriched, including the most common cancer‑associated forkhead box O signaling pathway (P=0.0077), microRNAs in cancer (P=0.0183) and the cAMP signaling pathway (P=0.0415). Furthermore, a network analysis identified three hub genes (HUWE1, PPP1CB and HSPA4), which were all involved in tumor metastasis. Taken together, the DEGs, significant pathways and hub genes identified in the present study shed some light on the molecular mechanisms of ENO1 involved in the pathogenesis of GC.
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Copy and paste a formatted citation
Spandidos Publications style
Huang Z, Lin B, Pan H, Du J, He R, Zhang S and Ouyang P: Gene expression profile analysis of ENO1 knockdown in gastric cancer cell line MGC‑803. Oncol Lett 17: 3881-3889, 2019.
APA
Huang, Z., Lin, B., Pan, H., Du, J., He, R., Zhang, S., & Ouyang, P. (2019). Gene expression profile analysis of ENO1 knockdown in gastric cancer cell line MGC‑803. Oncology Letters, 17, 3881-3889. https://doi.org/10.3892/ol.2019.10053
MLA
Huang, Z., Lin, B., Pan, H., Du, J., He, R., Zhang, S., Ouyang, P."Gene expression profile analysis of ENO1 knockdown in gastric cancer cell line MGC‑803". Oncology Letters 17.4 (2019): 3881-3889.
Chicago
Huang, Z., Lin, B., Pan, H., Du, J., He, R., Zhang, S., Ouyang, P."Gene expression profile analysis of ENO1 knockdown in gastric cancer cell line MGC‑803". Oncology Letters 17, no. 4 (2019): 3881-3889. https://doi.org/10.3892/ol.2019.10053
Copy and paste a formatted citation
x
Spandidos Publications style
Huang Z, Lin B, Pan H, Du J, He R, Zhang S and Ouyang P: Gene expression profile analysis of ENO1 knockdown in gastric cancer cell line MGC‑803. Oncol Lett 17: 3881-3889, 2019.
APA
Huang, Z., Lin, B., Pan, H., Du, J., He, R., Zhang, S., & Ouyang, P. (2019). Gene expression profile analysis of ENO1 knockdown in gastric cancer cell line MGC‑803. Oncology Letters, 17, 3881-3889. https://doi.org/10.3892/ol.2019.10053
MLA
Huang, Z., Lin, B., Pan, H., Du, J., He, R., Zhang, S., Ouyang, P."Gene expression profile analysis of ENO1 knockdown in gastric cancer cell line MGC‑803". Oncology Letters 17.4 (2019): 3881-3889.
Chicago
Huang, Z., Lin, B., Pan, H., Du, J., He, R., Zhang, S., Ouyang, P."Gene expression profile analysis of ENO1 knockdown in gastric cancer cell line MGC‑803". Oncology Letters 17, no. 4 (2019): 3881-3889. https://doi.org/10.3892/ol.2019.10053
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