Open Access

Effects of SIRT1 silencing on viability, invasion and metastasis of human glioma cell lines

  • Authors:
    • Yu Li
    • Xin Chen
    • Yong Cui
    • Qun Wei
    • Suiyun Chen
    • Xiaofang Wang
  • View Affiliations

  • Published online on: February 21, 2019     https://doi.org/10.3892/ol.2019.10063
  • Pages: 3701-3708
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Silent information regulator 1 (SIRT1), a member of the sirtuin family, is involved in the development of various types of tumor. Previous studies have revealed that SIRT1 has dual functions, as a promoter and an inhibitor, in certain tumors. However, the role of SIRT1 in invasion and metastasis of glioma cells and its associated signaling pathway remain unclear. The aim of the present study was to determine the effects of SIRT1 on these processes and on the epithelial‑mesenchymal transition (EMT) in human glioma and adjacent tissues, and in the human glioma cell lines U87 and U251. SIRT1 expression in tissues was investigated using the reverse transcription‑quantitative polymerase chain reaction, western blotting and immunohistochemistry. The U87 and U251 cell lines were divided into control and SIRT1‑small interfering RNA (siRNA) groups. The Cell Counting Kit‑8, cell invasion assays were used to evaluate the effects of SIRT1 silencing on cell viability, invasion and EMT. Results indicated that SIRT1 was highly expressed in glioma tissues compared with in adjacent brain tissues. In addition, SIRT1‑siRNA significantly inhibited the viability and invasion of U87 and U251 cells. Furthermore, EMT analysis revealed that the expression levels of the mesenchymal markers fibronectin and vimentin were significantly lower in the SIRT1‑siRNA group compared with in the control group. Conversely, expression levels of the epithelial markers epithelial cadherin and β‑catenin were significantly higher in the SIRT1‑siRNA group compared with in the control group. In conclusion, the results of the present study indicated that SIRT1 was positively associated with viability and invasion of U87 cells, potentially through EMT. These results suggested that SIRT1 may serve a crucial role in the proliferation and development of glioma.
View Figures
View References

Related Articles

Journal Cover

April-2019
Volume 17 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Chen X, Cui Y, Wei Q, Chen S and Wang X: Effects of SIRT1 silencing on viability, invasion and metastasis of human glioma cell lines. Oncol Lett 17: 3701-3708, 2019
APA
Li, Y., Chen, X., Cui, Y., Wei, Q., Chen, S., & Wang, X. (2019). Effects of SIRT1 silencing on viability, invasion and metastasis of human glioma cell lines. Oncology Letters, 17, 3701-3708. https://doi.org/10.3892/ol.2019.10063
MLA
Li, Y., Chen, X., Cui, Y., Wei, Q., Chen, S., Wang, X."Effects of SIRT1 silencing on viability, invasion and metastasis of human glioma cell lines". Oncology Letters 17.4 (2019): 3701-3708.
Chicago
Li, Y., Chen, X., Cui, Y., Wei, Q., Chen, S., Wang, X."Effects of SIRT1 silencing on viability, invasion and metastasis of human glioma cell lines". Oncology Letters 17, no. 4 (2019): 3701-3708. https://doi.org/10.3892/ol.2019.10063