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Article

Anti‑breast cancer potential of frullanolide from Grangea maderaspatana plant by inducing apoptosis

  • Authors:
    • Siriphorn Chimplee
    • Potchanapond Graidist
    • Theera Srisawat
    • Suchada Sukrong
    • Rassanee Bissanum
    • Kanyanatt Kanokwiroon
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand, Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand, Research Unit of DNA Barcoding of Thai Medicinal Plants, Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand
  • Pages: 5283-5291
    |
    Published online on: April 3, 2019
       https://doi.org/10.3892/ol.2019.10209
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Abstract

Breast cancer is the leading cause of female mortality worldwide. Although there are several modern treatments for breast cancer, there is a high rate of recurrence for the majority of treatments; therefore, the search for effective anticancer agents continues. The present study aimed to investigate the anti‑breast cancer potential of frullanolide, a compound which is isolated and purified from the Grangea maderaspatana plant, for selected human breast cancer cell lines (MCF‑7, MDA‑MB‑468 and MDA‑MB‑231). The MTT assay was used to assess cytotoxic activity in breast cancer cell lines of treatment with frullanolide at 1.25, 2.5, 5.0, 10.0 and 20.0 µg/ml. Additionally, the apoptotic induction ability of frullanolide at various concentrations [0.5x, 1x and 2x half maximal inhibitory concentration (IC50)] was investigated by flow cytometry and western blot analysis. Frullanolide exhibited strong anti‑breast cancer activity against MDA‑MB‑468 (IC50, 8.04±2.69 µg/ml) and weak cytotoxicity against the MCF‑7 (IC50, 10.74±0.86 µg/ml) and MDA‑MB‑231 (IC50, 12.36±0.31 µg/ml) cell lines. The IC50 of frullanolide was high in the human normal epithelial breast cell line (MCF‑12A) and mouse fibroblast cell line (L‑929). Density plot diagrams revealed that frullanolide induced apoptosis in MCF‑7, MDA‑MB‑468 and MDA‑MB‑231 cells. Notably, a plausible anticancer mechanism was elucidated via cellular apoptosis by p53‑independence in the treated MCF‑7 cell line and p53‑dependence in the treated MDA‑MB‑468 and MDA‑MB‑231 cell lines. In conclusion, the present study demonstrated that frullanolide may exert anticancer activity on breast cancer cell lines by inducing apoptosis. Frullanolide offers a possible novel approach to breast cancer therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Chimplee S, Graidist P, Srisawat T, Sukrong S, Bissanum R and Kanokwiroon K: Anti‑breast cancer potential of frullanolide from Grangea maderaspatana plant by inducing apoptosis . Oncol Lett 17: 5283-5291, 2019.
APA
Chimplee, S., Graidist, P., Srisawat, T., Sukrong, S., Bissanum, R., & Kanokwiroon, K. (2019). Anti‑breast cancer potential of frullanolide from Grangea maderaspatana plant by inducing apoptosis . Oncology Letters, 17, 5283-5291. https://doi.org/10.3892/ol.2019.10209
MLA
Chimplee, S., Graidist, P., Srisawat, T., Sukrong, S., Bissanum, R., Kanokwiroon, K."Anti‑breast cancer potential of frullanolide from Grangea maderaspatana plant by inducing apoptosis ". Oncology Letters 17.6 (2019): 5283-5291.
Chicago
Chimplee, S., Graidist, P., Srisawat, T., Sukrong, S., Bissanum, R., Kanokwiroon, K."Anti‑breast cancer potential of frullanolide from Grangea maderaspatana plant by inducing apoptosis ". Oncology Letters 17, no. 6 (2019): 5283-5291. https://doi.org/10.3892/ol.2019.10209
Copy and paste a formatted citation
x
Spandidos Publications style
Chimplee S, Graidist P, Srisawat T, Sukrong S, Bissanum R and Kanokwiroon K: Anti‑breast cancer potential of frullanolide from Grangea maderaspatana plant by inducing apoptosis . Oncol Lett 17: 5283-5291, 2019.
APA
Chimplee, S., Graidist, P., Srisawat, T., Sukrong, S., Bissanum, R., & Kanokwiroon, K. (2019). Anti‑breast cancer potential of frullanolide from Grangea maderaspatana plant by inducing apoptosis . Oncology Letters, 17, 5283-5291. https://doi.org/10.3892/ol.2019.10209
MLA
Chimplee, S., Graidist, P., Srisawat, T., Sukrong, S., Bissanum, R., Kanokwiroon, K."Anti‑breast cancer potential of frullanolide from Grangea maderaspatana plant by inducing apoptosis ". Oncology Letters 17.6 (2019): 5283-5291.
Chicago
Chimplee, S., Graidist, P., Srisawat, T., Sukrong, S., Bissanum, R., Kanokwiroon, K."Anti‑breast cancer potential of frullanolide from Grangea maderaspatana plant by inducing apoptosis ". Oncology Letters 17, no. 6 (2019): 5283-5291. https://doi.org/10.3892/ol.2019.10209
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