Fucoidan inhibits epithelial‑to‑mesenchymal transition via regulation of the HIF‑1α pathway in mammary cancer cells under hypoxia

Li,Weiwei; Xue,Dingshan; Xue,Meilan; Zhao,Jinglan; Liang,Hui; Liu,Ying; Sun,Ting

doi:10.3892/ol.2019.10283

Fucoidan inhibits epithelial‑to‑mesenchymal transition via regulation of the HIF‑1α pathway in mammary cancer cells under hypoxia

Authors:
- Weiwei Li
- Dingshan Xue
- Meilan Xue
- Jinglan Zhao
- Hui Liang
- Ying Liu
- Ting Sun
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Affiliations: Department of Biochemistry and Molecular Biology, Basic Medical College, Qingdao University of Medicine, Qingdao, Shandong 266021, P.R. China, Department of Senior Grade Three, Qingdao West Coast District No. 1 Senior High School, Qingdao, Shandong 266555, P.R. China, Department of Cardiothoracic Surgery of Qingdao Center Medical Group, Qingdao, Shandong 266042, P.R. China, The Institute of Human Nutrition, Qingdao University of Medicine, Qingdao, Shandong 266021, P.R. China
Published online on: April 25, 2019 https://doi.org/10.3892/ol.2019.10283
Pages: 330-338

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Abstract

This study examined the effects of fucoidan on epithelial‑to‑mesenchymal transition (EMT) in a human triple‑negative breast cancer (TNBC) cell line in a hypoxic microenvironment. Transwell and wound‑healing assays were performed to analyze the invasion and migration of MDA‑MB‑231 human mammary cancer cells, respectively. The expression levels of EMT markers and hypoxia‑inducible factor‑1α (HIF‑1α) were detected through western blotting. Under hypoxia, fucoidan treatment inhibited proliferation of breast cancer cells. Fucoidan also suppressed the invasion and migration of MDA‑MB‑231 cells. Western blotting revealed that fucoidan treatment significantly reduced the protein expression levels of HIF‑1α and HIF‑1 target genes. Furthermore, the nuclear translocation and activity of HIF‑1α were reduced. Fucoidan treatment significantly downregulated the expression levels of mesenchymal markers (N‑cadherin and vimentin), but upregulated the expression levels of the epithelial markers zonula occludens‑1 and E‑cadherin. In addition, overexpression of HIF1‑α protected cells from fucoidan‑mediated suppression of migration and invasion. These data suggested that fucoidan may inhibit EMT in human TNBC cells via downregulation of the HIF1‑α signaling pathway.

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