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Article Open Access

Ampelopsin‑sodium induces apoptosis in human lung adenocarcinoma cell lines by promoting tubulin polymerization in vitro

  • Authors:
    • Lijuan Zhu
    • Baolai Zhang
    • Jianyun Luo
    • Shuhong Dong
    • Kaihong Zang
    • Yongjie Wu
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, School of Basic Medical Science, Lanzhou University, Lanzhou, Gansu 730000, P.R. China, Department of Drug Policy and Essential Medicine, Xi'an Municipal Health Commission, Xi'an, Shaanxi 710000, P.R. China, Department of Pharmacology, College of Pharmacy, Gansu University of Traditional Chinese Medicine, Lanzhou, Gansu 730000, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 189-196
    |
    Published online on: April 30, 2019
       https://doi.org/10.3892/ol.2019.10288
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Abstract

Previous studies have demonstrated that ampelopsin (AMP), a type of flavonoid isolated from the stems and leaves of Ampelopsis grossedentata, exhibits anti‑cancer activity in various types of cancer. Conversion of AMP into its sodium salt (AMP‑Na) conferred enhanced solubility and stability to it. The present study aimed to evaluate the anti‑cancer activity of AMP‑Na in human lung adenocarcinoma cell lines and to investigate its mechanisms of action. Cell proliferation and viability were assessed by MTT and colony formation assays, and cell migration was determined using a scratch wound healing assay. The cell cycle distribution, apoptosis rate and tubulin immunofluorescence intensity were analyzed using flow cytometry, the cell ultra‑microstructure was examined using transmission electron microscopy and the accumulation of tubulin was determined using laser confocal microscopy. The results demonstrated that AMP‑Na significantly inhibited the proliferation, clonogenicity and migration of human lung adenocarcinoma cells. Furthermore, AMP‑Na induced SPC‑A‑1 cell apoptosis, and promoted tubulin polymerization. The results suggested that the underlying mechanisms of AMP‑Na may involve targeting of microtubules and tubulin polymerization to subsequently disrupt mitosis and induce cell cycle arrest at the S‑phase.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu L, Zhang B, Luo J, Dong S, Zang K and Wu Y: Ampelopsin‑sodium induces apoptosis in human lung adenocarcinoma cell lines by promoting tubulin polymerization in vitro. Oncol Lett 18: 189-196, 2019.
APA
Zhu, L., Zhang, B., Luo, J., Dong, S., Zang, K., & Wu, Y. (2019). Ampelopsin‑sodium induces apoptosis in human lung adenocarcinoma cell lines by promoting tubulin polymerization in vitro. Oncology Letters, 18, 189-196. https://doi.org/10.3892/ol.2019.10288
MLA
Zhu, L., Zhang, B., Luo, J., Dong, S., Zang, K., Wu, Y."Ampelopsin‑sodium induces apoptosis in human lung adenocarcinoma cell lines by promoting tubulin polymerization in vitro". Oncology Letters 18.1 (2019): 189-196.
Chicago
Zhu, L., Zhang, B., Luo, J., Dong, S., Zang, K., Wu, Y."Ampelopsin‑sodium induces apoptosis in human lung adenocarcinoma cell lines by promoting tubulin polymerization in vitro". Oncology Letters 18, no. 1 (2019): 189-196. https://doi.org/10.3892/ol.2019.10288
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu L, Zhang B, Luo J, Dong S, Zang K and Wu Y: Ampelopsin‑sodium induces apoptosis in human lung adenocarcinoma cell lines by promoting tubulin polymerization in vitro. Oncol Lett 18: 189-196, 2019.
APA
Zhu, L., Zhang, B., Luo, J., Dong, S., Zang, K., & Wu, Y. (2019). Ampelopsin‑sodium induces apoptosis in human lung adenocarcinoma cell lines by promoting tubulin polymerization in vitro. Oncology Letters, 18, 189-196. https://doi.org/10.3892/ol.2019.10288
MLA
Zhu, L., Zhang, B., Luo, J., Dong, S., Zang, K., Wu, Y."Ampelopsin‑sodium induces apoptosis in human lung adenocarcinoma cell lines by promoting tubulin polymerization in vitro". Oncology Letters 18.1 (2019): 189-196.
Chicago
Zhu, L., Zhang, B., Luo, J., Dong, S., Zang, K., Wu, Y."Ampelopsin‑sodium induces apoptosis in human lung adenocarcinoma cell lines by promoting tubulin polymerization in vitro". Oncology Letters 18, no. 1 (2019): 189-196. https://doi.org/10.3892/ol.2019.10288
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