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Article Open Access

ERCC6L promotes cell growth and invasion in human colorectal cancer

  • Authors:
    • Yang Xie
    • Jun Yu
    • Feng Wang
    • Mengying Li
    • Xiao Qiu
    • Yuting Liu
    • Jian Qi
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China
    Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 237-246
    |
    Published online on: April 30, 2019
       https://doi.org/10.3892/ol.2019.10297
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Abstract

Excision repair cross‑complementation group 6 like (ERCC6L), a recently discovered DNA helicase, has been demonstrated to be highly expressed in a variety of human cancer types. However, the precise role of ERCC6L in colorectal cancer (CRC) remains unclear. The current study aimed to investigate the potential role of ERCC6L in the development and progression of CRC. Reverse transcription‑quantitative polymerase chain reaction, western blot analysis and immunohistochemistry were used to detect the expression level of ERCC6L in 30 matched pairs of CRC and adjacent noncancerous tissues. The function of ERCC6L in cell proliferation, cycle, apoptosis, invasion and colony formation was examined in CRC cell lines. ERCC6L was revealed to be highly expressed in CRC tissues and cell lines compared with normal controls (P<0.05). The expression level of ERCC6L was significantly associated with tumor size (P<0.05), but not with other clinical features, including age, gender, differentiation and clinical stage. It was identified that reducing ERCC6L expression using small interfering RNA significantly inhibited the proliferation and colony‑forming ability of CRC cell lines. Flow cytometric analysis demonstrated that ERCC6L knockdown in CRC cells inhibited cell cycle progression and increased the number of cells in the G0/G1 phase without affecting apoptosis. Furthermore, ERCC6L knockdown markedly decreased the number of invading CRC cells compared with control cells. These results suggest that ERCC6L promotes the growth and invasion of CRC cells, and ERCC6L may be a potential new target for cancer therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Xie Y, Yu J, Wang F, Li M, Qiu X, Liu Y and Qi J: ERCC6L promotes cell growth and invasion in human colorectal cancer. Oncol Lett 18: 237-246, 2019.
APA
Xie, Y., Yu, J., Wang, F., Li, M., Qiu, X., Liu, Y., & Qi, J. (2019). ERCC6L promotes cell growth and invasion in human colorectal cancer. Oncology Letters, 18, 237-246. https://doi.org/10.3892/ol.2019.10297
MLA
Xie, Y., Yu, J., Wang, F., Li, M., Qiu, X., Liu, Y., Qi, J."ERCC6L promotes cell growth and invasion in human colorectal cancer". Oncology Letters 18.1 (2019): 237-246.
Chicago
Xie, Y., Yu, J., Wang, F., Li, M., Qiu, X., Liu, Y., Qi, J."ERCC6L promotes cell growth and invasion in human colorectal cancer". Oncology Letters 18, no. 1 (2019): 237-246. https://doi.org/10.3892/ol.2019.10297
Copy and paste a formatted citation
x
Spandidos Publications style
Xie Y, Yu J, Wang F, Li M, Qiu X, Liu Y and Qi J: ERCC6L promotes cell growth and invasion in human colorectal cancer. Oncol Lett 18: 237-246, 2019.
APA
Xie, Y., Yu, J., Wang, F., Li, M., Qiu, X., Liu, Y., & Qi, J. (2019). ERCC6L promotes cell growth and invasion in human colorectal cancer. Oncology Letters, 18, 237-246. https://doi.org/10.3892/ol.2019.10297
MLA
Xie, Y., Yu, J., Wang, F., Li, M., Qiu, X., Liu, Y., Qi, J."ERCC6L promotes cell growth and invasion in human colorectal cancer". Oncology Letters 18.1 (2019): 237-246.
Chicago
Xie, Y., Yu, J., Wang, F., Li, M., Qiu, X., Liu, Y., Qi, J."ERCC6L promotes cell growth and invasion in human colorectal cancer". Oncology Letters 18, no. 1 (2019): 237-246. https://doi.org/10.3892/ol.2019.10297
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