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Radiosensitization effects of curcumin plus cisplatin on non‑small cell lung cancer A549 cells

  • Authors:
    • Yong Cai
    • Zhaoying Sheng
    • Shixiong Liang
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, P.R. China
    Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 529-534
    |
    Published online on: May 17, 2019
       https://doi.org/10.3892/ol.2019.10364
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Abstract

The aim of the present study was to determine the radiosensitization effect of the combination of curcumin and cisplatin on non‑small cell lung cancer (NSCLC) A549 cells. Cell viability was analyzed using the MTT assay following treatment with different concentrations of curcumin and cisplatin for 24~72 h. Survival fraction (SF) value of the treatment groups (single irradiation, curcumin + irradiation, cisplatin + irradiation, and curcumin + cisplatin + irradiation) treated with different doses of X‑ray radiation were evaluated using colony formation assay, according to a multi‑target single‑hit model. Migration and invasion as well as the levels of epidermal growth factor receptor (EGFR) protein following 24 h were detected by scratch wound assay, Matrigel assay and western blot analysis, respectively. The results of the present study demonstrated that the viability of the cells decreased after being treated by curcumin, and the inhibitory effect was dose and time‑dependent as the concentration of curcumin increased from 10 to 200 µmol/l (P<0.05). SF value was lower in the curcumin + cisplatin + irradiation group compared with the other three treatment groups at 2~10 Gy. Furthermore, SF value was lower in the curcumin + irradiation group at 4~10 Gy. The SF value was also lower in the cisplatin + irradiation group at 2~10 Gy compared with the single irradiation group (P<0.05). The sensitization enhancement ratios in the curcumin + irradiation, cisplatin + irradiation, and curcumin + cisplatin + irradiation groups were 1.24, 1.31 and 1.96, respectively. The migration distance, the number of cells invaded through the transmembrane, and the level of EGFR protein in four treatment groups were the highest in the single irradiation group, compared with the other three treatment groups (P<0.05). Furthermore, the radiosensitization effects of curcumin and cisplatin on NSCLC A549 cells, which include inhibition of proliferation, migration and invasion, may be associated with the inhibition of the EGFR‑associated signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Cai Y, Sheng Z and Liang S: Radiosensitization effects of curcumin plus cisplatin on non‑small cell lung cancer A549 cells. Oncol Lett 18: 529-534, 2019.
APA
Cai, Y., Sheng, Z., & Liang, S. (2019). Radiosensitization effects of curcumin plus cisplatin on non‑small cell lung cancer A549 cells. Oncology Letters, 18, 529-534. https://doi.org/10.3892/ol.2019.10364
MLA
Cai, Y., Sheng, Z., Liang, S."Radiosensitization effects of curcumin plus cisplatin on non‑small cell lung cancer A549 cells". Oncology Letters 18.1 (2019): 529-534.
Chicago
Cai, Y., Sheng, Z., Liang, S."Radiosensitization effects of curcumin plus cisplatin on non‑small cell lung cancer A549 cells". Oncology Letters 18, no. 1 (2019): 529-534. https://doi.org/10.3892/ol.2019.10364
Copy and paste a formatted citation
x
Spandidos Publications style
Cai Y, Sheng Z and Liang S: Radiosensitization effects of curcumin plus cisplatin on non‑small cell lung cancer A549 cells. Oncol Lett 18: 529-534, 2019.
APA
Cai, Y., Sheng, Z., & Liang, S. (2019). Radiosensitization effects of curcumin plus cisplatin on non‑small cell lung cancer A549 cells. Oncology Letters, 18, 529-534. https://doi.org/10.3892/ol.2019.10364
MLA
Cai, Y., Sheng, Z., Liang, S."Radiosensitization effects of curcumin plus cisplatin on non‑small cell lung cancer A549 cells". Oncology Letters 18.1 (2019): 529-534.
Chicago
Cai, Y., Sheng, Z., Liang, S."Radiosensitization effects of curcumin plus cisplatin on non‑small cell lung cancer A549 cells". Oncology Letters 18, no. 1 (2019): 529-534. https://doi.org/10.3892/ol.2019.10364
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