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Article Open Access

SNHG20/miR‑140‑5p/NDRG3 axis contributes to 5‑fluorouracil resistance in gastric cancer

  • Authors:
    • Jie Yu
    • Jie Shen
    • Xu Qiao
    • Longlei Cao
    • Zhangling Yang
    • Hui Ye
    • Changlei Xi
    • Qichang Zhou
    • Peiyun Wang
    • Zhilin Gong
  • View Affiliations / Copyright

    Affiliations: Department of Colorectal and Anal Surgery, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, Hubei 434020, P.R. China, Department of Digestive Endoscopy, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, Hubei 434020, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1337-1343
    |
    Published online on: June 5, 2019
       https://doi.org/10.3892/ol.2019.10439
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Abstract

5‑fluorouracil (5‑FU)‑based chemotherapy is the first line treatment for advanced gastric cancer. However, the effectiveness of 5‑FU is limited by drug resistance. The N‑myc downstream‑regulated gene, family member 3 (NDRG3) is a member of the NDRG family and has been implicated in numerous types of cancer. However, the role of NDRG3 in gastric cancer remains unclear. In the present study, NDRG3 mRNA expression in gastric cancer and adjacent normal tissues was analyzed using the Gene Expression Profiling Interactive Analysis web tool. NDRG3 expression was silenced using short hairpin RNAs to examine the effect of NDRG3 on the growth of gastric cancer cells. Potential regulators of NDRG3 were identified using the TargetScan and MicroRNA tools and verified by a luciferase assay and reverse transcription‑quantitative PCR analysis. The current study demonstrated that NDRG3 was upregulated in gastric cancer specimens and promoted cell proliferation in gastric cancer cell lines. Furthermore, the present study revealed that the small nucleolar RNA host gene 20 (SNHG20)/microRNA (miR)‑140‑5p signaling pathway may regulate the expression of NDRG3. SNHG20 was revealed to be involved in mediating resistance to 5‑FU in gastric cancer cell lines via NDRG3. In conclusion, the results of the present study suggest that the SNHG20/miR‑140‑5p/NDRG3 axis may be involved in mediating resistance to 5‑FU in gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Yu J, Shen J, Qiao X, Cao L, Yang Z, Ye H, Xi C, Zhou Q, Wang P, Gong Z, Gong Z, et al: SNHG20/miR‑140‑5p/NDRG3 axis contributes to 5‑fluorouracil resistance in gastric cancer. Oncol Lett 18: 1337-1343, 2019.
APA
Yu, J., Shen, J., Qiao, X., Cao, L., Yang, Z., Ye, H. ... Gong, Z. (2019). SNHG20/miR‑140‑5p/NDRG3 axis contributes to 5‑fluorouracil resistance in gastric cancer. Oncology Letters, 18, 1337-1343. https://doi.org/10.3892/ol.2019.10439
MLA
Yu, J., Shen, J., Qiao, X., Cao, L., Yang, Z., Ye, H., Xi, C., Zhou, Q., Wang, P., Gong, Z."SNHG20/miR‑140‑5p/NDRG3 axis contributes to 5‑fluorouracil resistance in gastric cancer". Oncology Letters 18.2 (2019): 1337-1343.
Chicago
Yu, J., Shen, J., Qiao, X., Cao, L., Yang, Z., Ye, H., Xi, C., Zhou, Q., Wang, P., Gong, Z."SNHG20/miR‑140‑5p/NDRG3 axis contributes to 5‑fluorouracil resistance in gastric cancer". Oncology Letters 18, no. 2 (2019): 1337-1343. https://doi.org/10.3892/ol.2019.10439
Copy and paste a formatted citation
x
Spandidos Publications style
Yu J, Shen J, Qiao X, Cao L, Yang Z, Ye H, Xi C, Zhou Q, Wang P, Gong Z, Gong Z, et al: SNHG20/miR‑140‑5p/NDRG3 axis contributes to 5‑fluorouracil resistance in gastric cancer. Oncol Lett 18: 1337-1343, 2019.
APA
Yu, J., Shen, J., Qiao, X., Cao, L., Yang, Z., Ye, H. ... Gong, Z. (2019). SNHG20/miR‑140‑5p/NDRG3 axis contributes to 5‑fluorouracil resistance in gastric cancer. Oncology Letters, 18, 1337-1343. https://doi.org/10.3892/ol.2019.10439
MLA
Yu, J., Shen, J., Qiao, X., Cao, L., Yang, Z., Ye, H., Xi, C., Zhou, Q., Wang, P., Gong, Z."SNHG20/miR‑140‑5p/NDRG3 axis contributes to 5‑fluorouracil resistance in gastric cancer". Oncology Letters 18.2 (2019): 1337-1343.
Chicago
Yu, J., Shen, J., Qiao, X., Cao, L., Yang, Z., Ye, H., Xi, C., Zhou, Q., Wang, P., Gong, Z."SNHG20/miR‑140‑5p/NDRG3 axis contributes to 5‑fluorouracil resistance in gastric cancer". Oncology Letters 18, no. 2 (2019): 1337-1343. https://doi.org/10.3892/ol.2019.10439
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