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Article

Increased frequency of regulatory T cells in the peripheral blood of patients with endometrioid adenocarcinoma

  • Authors:
    • Li Li
    • Yinghua Li
    • Zhuomin Yin
    • Jing Zhu
    • Dingding Yan
    • Hanmei Lou
  • View Affiliations / Copyright

    Affiliations: Department of Gynecologic Oncology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, P.R. China
  • Pages: 1424-1430
    |
    Published online on: June 7, 2019
       https://doi.org/10.3892/ol.2019.10452
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Abstract

The aim of the present study was to investigate the frequency of cluster of differentiation (CD)4+CD25+CD127‑ regulatory T cells (Tregs) in the peripheral blood mononuclear cells (PBMCs) of patients with endometrioid adenocarcinoma (EA). A total of 82 female patients with EA were recruited. The PBMCs were flow cytometrically analyzed to determine the percentage of CD4+CD25+CD127‑ Tregs within the CD4+ T cell population. The associations between the prevalence of Tregs in PBMCs and defined clinical prognostic parameters were evaluated. To study the immunoregulatory capacity of Tregs, the level of specific cytokines were detected by ELISA, and the proliferation of cells was analyzed by incorporation of 3H‑thymidine. It was revealed that Treg/CD4+ ratio in the peripheral blood of patients with EA was 4.89±1.42%, significantly higher than Treg/CD4+ ratio in healthy women. No correlation was observed between Treg frequency and stage, grade of differentiation, menopausal status or age. CD4+CD25+CD127‑ Tregs secreted large amounts of IL‑10 but not IFN‑γ. The level of IL‑10 secreted by Tregs from patients with EA and healthy controls was not significantly different. In addition, there was no significant difference in the suppressive activity of Tregs in patients with EA compared with that of the healthy controls. These findings demonstrate that the increased frequency of immunosuppressive Tregs in patients with EA may be responsible for immune tolerance in endometrial cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Li L, Li Y, Yin Z, Zhu J, Yan D and Lou H: Increased frequency of regulatory T cells in the peripheral blood of patients with endometrioid adenocarcinoma . Oncol Lett 18: 1424-1430, 2019.
APA
Li, L., Li, Y., Yin, Z., Zhu, J., Yan, D., & Lou, H. (2019). Increased frequency of regulatory T cells in the peripheral blood of patients with endometrioid adenocarcinoma . Oncology Letters, 18, 1424-1430. https://doi.org/10.3892/ol.2019.10452
MLA
Li, L., Li, Y., Yin, Z., Zhu, J., Yan, D., Lou, H."Increased frequency of regulatory T cells in the peripheral blood of patients with endometrioid adenocarcinoma ". Oncology Letters 18.2 (2019): 1424-1430.
Chicago
Li, L., Li, Y., Yin, Z., Zhu, J., Yan, D., Lou, H."Increased frequency of regulatory T cells in the peripheral blood of patients with endometrioid adenocarcinoma ". Oncology Letters 18, no. 2 (2019): 1424-1430. https://doi.org/10.3892/ol.2019.10452
Copy and paste a formatted citation
x
Spandidos Publications style
Li L, Li Y, Yin Z, Zhu J, Yan D and Lou H: Increased frequency of regulatory T cells in the peripheral blood of patients with endometrioid adenocarcinoma . Oncol Lett 18: 1424-1430, 2019.
APA
Li, L., Li, Y., Yin, Z., Zhu, J., Yan, D., & Lou, H. (2019). Increased frequency of regulatory T cells in the peripheral blood of patients with endometrioid adenocarcinoma . Oncology Letters, 18, 1424-1430. https://doi.org/10.3892/ol.2019.10452
MLA
Li, L., Li, Y., Yin, Z., Zhu, J., Yan, D., Lou, H."Increased frequency of regulatory T cells in the peripheral blood of patients with endometrioid adenocarcinoma ". Oncology Letters 18.2 (2019): 1424-1430.
Chicago
Li, L., Li, Y., Yin, Z., Zhu, J., Yan, D., Lou, H."Increased frequency of regulatory T cells in the peripheral blood of patients with endometrioid adenocarcinoma ". Oncology Letters 18, no. 2 (2019): 1424-1430. https://doi.org/10.3892/ol.2019.10452
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