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Tumor‑associated macrophages affect the biological behavior of lung adenocarcinoma A549 cells through the PI3K/AKT signaling pathway

  • Authors:
    • Shiyang Yuan
    • Yaling Dong
    • Laishui Peng
    • Mei Yang
    • Linxia Niu
    • Zhiwen Liu
    • Junping Xie
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1840-1846
    |
    Published online on: June 14, 2019
       https://doi.org/10.3892/ol.2019.10483
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Abstract

Tumor associated macrophages (TAMs) are a major type of inflammatory cell in a tumor microenvironment. Previous evidence has suggested that TAMs promote tumorigenesis, growth, invasion and metastasis, thereby affecting tumor metabolism. The mechanisms through which they affect the invasion and metastasis of lung cancer cells remain unclear. The present study investigated the effects and molecular mechanisms of TAMs on the proliferation, invasion and migration of lung adenocarcinoma A549 cells. Human mononuclear leukemia THP‑1 cells were induced into TAMs. The morphological changes that occurred during the induction of the THP‑1 cells were examined with a light microscope. Successful TAM formation was confirmed via flow cytometry. Proliferation, invasion and migration of the lung adenocarcinoma A549 cells were detected by EDU proliferation, scratch wound and Transwell invasion and migration assays, respectively. The expression levels of key proteins involved in the PI3K/AKT signaling pathway were detected by western blot analysis. It was identified that treatment with interleukin (IL)‑4, IL‑13 and Phorbol‑12‑myristate‑13‑acetate successfully induced THP‑1 to form TAMs. The indirect coculture model of TAMs was established by Transwell chamber detection, and the proliferation, invasion and migration ability of lung adenocarcinoma A549 cells were enhanced. Western blot analysis indicated that the expression levels of phosphorylated (p)‑PI3K and p‑AKT proteins were significantly upregulated in the TAMs coculture group compared with that of the blank control group. In summary, the present study demonstrated that TAMs may promote the proliferation, invasion and migration of lung adenocarcinoma A549 cells in vitro, perhaps through the activation of the PI3K/AKT signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Yuan S, Dong Y, Peng L, Yang M, Niu L, Liu Z and Xie J: Tumor‑associated macrophages affect the biological behavior of lung adenocarcinoma A549 cells through the PI3K/AKT signaling pathway. Oncol Lett 18: 1840-1846, 2019.
APA
Yuan, S., Dong, Y., Peng, L., Yang, M., Niu, L., Liu, Z., & Xie, J. (2019). Tumor‑associated macrophages affect the biological behavior of lung adenocarcinoma A549 cells through the PI3K/AKT signaling pathway. Oncology Letters, 18, 1840-1846. https://doi.org/10.3892/ol.2019.10483
MLA
Yuan, S., Dong, Y., Peng, L., Yang, M., Niu, L., Liu, Z., Xie, J."Tumor‑associated macrophages affect the biological behavior of lung adenocarcinoma A549 cells through the PI3K/AKT signaling pathway". Oncology Letters 18.2 (2019): 1840-1846.
Chicago
Yuan, S., Dong, Y., Peng, L., Yang, M., Niu, L., Liu, Z., Xie, J."Tumor‑associated macrophages affect the biological behavior of lung adenocarcinoma A549 cells through the PI3K/AKT signaling pathway". Oncology Letters 18, no. 2 (2019): 1840-1846. https://doi.org/10.3892/ol.2019.10483
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan S, Dong Y, Peng L, Yang M, Niu L, Liu Z and Xie J: Tumor‑associated macrophages affect the biological behavior of lung adenocarcinoma A549 cells through the PI3K/AKT signaling pathway. Oncol Lett 18: 1840-1846, 2019.
APA
Yuan, S., Dong, Y., Peng, L., Yang, M., Niu, L., Liu, Z., & Xie, J. (2019). Tumor‑associated macrophages affect the biological behavior of lung adenocarcinoma A549 cells through the PI3K/AKT signaling pathway. Oncology Letters, 18, 1840-1846. https://doi.org/10.3892/ol.2019.10483
MLA
Yuan, S., Dong, Y., Peng, L., Yang, M., Niu, L., Liu, Z., Xie, J."Tumor‑associated macrophages affect the biological behavior of lung adenocarcinoma A549 cells through the PI3K/AKT signaling pathway". Oncology Letters 18.2 (2019): 1840-1846.
Chicago
Yuan, S., Dong, Y., Peng, L., Yang, M., Niu, L., Liu, Z., Xie, J."Tumor‑associated macrophages affect the biological behavior of lung adenocarcinoma A549 cells through the PI3K/AKT signaling pathway". Oncology Letters 18, no. 2 (2019): 1840-1846. https://doi.org/10.3892/ol.2019.10483
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