Integrated miRNA profiling and bioinformatics analyses reveal upregulated miRNAs in gastric cancer

Yuan,Chen; Zhang,Yue; Tu,Wenwen; Guo,Yusheng

doi:10.3892/ol.2019.10495

Integrated miRNA profiling and bioinformatics analyses reveal upregulated miRNAs in gastric cancer

Authors:
- Chen Yuan
- Yue Zhang
- Wenwen Tu
- Yusheng Guo
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Affiliations: Department of Emergency, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China, Department of Gerontology, Shanghai Jiaotong University Affiliated to Sixth People's Hospital, Shanghai 201499, P.R. China, Department of Cardiology, Jingan District Zhabei Central Hospital, Shanghai 200040, P.R. China
Published online on: June 19, 2019 https://doi.org/10.3892/ol.2019.10495
Pages: 1979-1988

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Abstract

Gastric cancer is one of the most common malignancies in China and exhibits a poor prognosis. The most significant challenge for gastric cancer treatment is the absence of early diagnostic biomarkers. MicroRNAs (miRNAs) are small non‑coding RNAs, which possess clinical value in a number of different types of cancer. The current study identified 13 miRNAs (hsa‑miR‑22, hsa‑miR‑545, hsa‑let‑7i, hsa‑miR‑15b, hsa‑miR‑221, hsa‑miR‑196a, hsa‑miR‑20a, hsa‑miR‑196b, hsa‑miR‑93, hsa‑miR‑19a, hsa‑miR‑503, hsa‑miR‑106b and hsa‑miR‑18a) that were significantly overexpressed in GC, by analyzing 1,000 GC samples included in four public datasets, including GSE23739, GSE78091, GSE30070 and The Cancer Genome Atlas. Furthermore, it was revealed that the expression levels of these 13 miRNAs were significantly higher in gastric cancer tissues of grades I, II and III compared with normal controls. Gene ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis demonstrated that the differentially expressed miRNAs were involved in regulating transcription, protein amino acid phosphorylation, signal transduction, protein binding, zinc ion binding, the mitogen‑activated protein kinase signaling pathway and focal adhesion. In summary, the present study may provide potential new therapeutic and prognostic targets for gastric cancer.

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