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Article Open Access

ILF2 promotes anchorage independence through direct regulation of PTEN

  • Authors:
    • Na Li
    • Tao Liu
    • Hui Li
    • Lifang Zhang
    • Liping Chu
    • Qingge Meng
    • Qinzeng Qiao
    • Weikun Han
    • Junhui Zhang
    • Minying Guo
    • Jia Zhao
  • View Affiliations / Copyright

    Affiliations: Pathology Department, Xingtai Medical College, Xingtai, Hebei 054000, P.R. China, Department of Internal Medicine, Civil Administration General Hospital of Hebei, Xingtai, Hebei 054000, P.R. China, Department of Internal Medicine, Xingtai Medical College, The Third Affiliated Hospital of Xingtai Medical College, Xingtai, Hebei 054000, P.R. China, Department of Internal Medicine, The Third Affiliated Hospital of Xingtai Medical College, Xingtai, Hebei 054000, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1689-1696
    |
    Published online on: June 21, 2019
       https://doi.org/10.3892/ol.2019.10510
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Abstract

Anoikis is a specific form of programmed cell death induced by loss of contact between cells and extracellular matrices or other cells. Only tumor cells that are resistant to anoikis can survive in the state of detachment from the primary tissue during the early stages of metastasis. The ability to resist anoikis is crucial for cancer cell metastasis. ILF2 is a proto‑oncogene previously studied in glioma, NSCLC, esophageal cancer and pancreatic ductal carcinoma. The results from the present study revealed that the transcription factor interleukin enhancer‑binding factor 2 (ILF2) was highly expressed in non‑small cell lung cancer (NSCLC) cell lines compared with in normal cell lines. ChIP and luciferase reporter gene assays demonstrated that ILF2 inhibited the expression level of the tumor suppressor gene phosphatase and tensin homolog (PTEN) by directly binding to its upstream regulatory region. Furthermore, the results from the detection of cell adhesion and apoptosis in cell suspension culture demonstrated that this mechanism enabled NSCLC cells to reduce adherence to the matrix and to survive in this abnormal state. These results suggested that ILF2 may promote the anchorage‑independence of NSCLC cells through the suppression of PTEN.
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Copy and paste a formatted citation
Spandidos Publications style
Li N, Liu T, Li H, Zhang L, Chu L, Meng Q, Qiao Q, Han W, Zhang J, Guo M, Guo M, et al: ILF2 promotes anchorage independence through direct regulation of PTEN. Oncol Lett 18: 1689-1696, 2019.
APA
Li, N., Liu, T., Li, H., Zhang, L., Chu, L., Meng, Q. ... Zhao, J. (2019). ILF2 promotes anchorage independence through direct regulation of PTEN. Oncology Letters, 18, 1689-1696. https://doi.org/10.3892/ol.2019.10510
MLA
Li, N., Liu, T., Li, H., Zhang, L., Chu, L., Meng, Q., Qiao, Q., Han, W., Zhang, J., Guo, M., Zhao, J."ILF2 promotes anchorage independence through direct regulation of PTEN". Oncology Letters 18.2 (2019): 1689-1696.
Chicago
Li, N., Liu, T., Li, H., Zhang, L., Chu, L., Meng, Q., Qiao, Q., Han, W., Zhang, J., Guo, M., Zhao, J."ILF2 promotes anchorage independence through direct regulation of PTEN". Oncology Letters 18, no. 2 (2019): 1689-1696. https://doi.org/10.3892/ol.2019.10510
Copy and paste a formatted citation
x
Spandidos Publications style
Li N, Liu T, Li H, Zhang L, Chu L, Meng Q, Qiao Q, Han W, Zhang J, Guo M, Guo M, et al: ILF2 promotes anchorage independence through direct regulation of PTEN. Oncol Lett 18: 1689-1696, 2019.
APA
Li, N., Liu, T., Li, H., Zhang, L., Chu, L., Meng, Q. ... Zhao, J. (2019). ILF2 promotes anchorage independence through direct regulation of PTEN. Oncology Letters, 18, 1689-1696. https://doi.org/10.3892/ol.2019.10510
MLA
Li, N., Liu, T., Li, H., Zhang, L., Chu, L., Meng, Q., Qiao, Q., Han, W., Zhang, J., Guo, M., Zhao, J."ILF2 promotes anchorage independence through direct regulation of PTEN". Oncology Letters 18.2 (2019): 1689-1696.
Chicago
Li, N., Liu, T., Li, H., Zhang, L., Chu, L., Meng, Q., Qiao, Q., Han, W., Zhang, J., Guo, M., Zhao, J."ILF2 promotes anchorage independence through direct regulation of PTEN". Oncology Letters 18, no. 2 (2019): 1689-1696. https://doi.org/10.3892/ol.2019.10510
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