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In vitro evaluation of anti‑epidermoid cancer activity of Acanthus ebracteatus protein hydrolysate and their effects on apoptosis and cellular proteins

  • Authors:
    • Ariya Khamwut
    • Damita Jevapatarakul
    • Onrapak Reamtong
    • Nattanan Panjaworayan T‑Thienprasert
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand, Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
    Copyright: © Khamwut et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3128-3136
    |
    Published online on: July 22, 2019
       https://doi.org/10.3892/ol.2019.10647
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Abstract

Acanthus ebracteatus Vahl. is commonly consumed with the aim of curing cancer, inflammatory conditions and skin diseases in traditional Thai medicine. It is known to contain various phytochemicals; however, very little is known about the effects of A. ebracteatus protein hydrolysate on cancer cells, including its molecular mechanisms. The present study therefore investigated the anti‑cancer activity of A. ebracteatus protein hydrolysates against epidermoid cancer of the skin cell line A431. Their effects on the apoptosis pathway and expression of proteins involved in the regulation of apoptosis, cell proliferation or cell cycle were also investigated. Crude extract of protein hydrolysate, partially purified peptides and purified peptides extracted from the aerial part of A. ebracteatus were administered to the A431 cells. The cytotoxicity effects were then determined using an MTT assay. As a result, A. ebracteatus protein hydrolysate significantly inhibited A431 cells with half inhibitory concentration equals to 425.9 ng protein/ml. By performing Annexin V assay, the partially purified peptides of A. ebracteatus were demonstrated to enhance the apoptosis pathway. Furthermore, western blot analysis revealed that the partially purified peptides of A. ebracteatus increased protein expression levels of RelA (p65) and Cyclin D1 proteins. However, A. ebracteatus did not increase the expression levels of p53‑serine 15 phosphorylation (Ser15P).
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1 

Subudhi HN, Choudhury BP and Acharya BC: Potential medicinal plants from Mahanadi delta in the state of Orissa. J Econ Taxon Bot. 16:479–487. 1992.

2 

Perry LM and Metzger J: Medicinal plants of East and Southeast Asia: Attributed properties and uses. Bibliography. 2:447–493. 1980.

3 

Laupattarakasem P, Houghton PJ, Hoult JR and Itharat A: An evaluation of the activity related to inflammation of four plants used in Thailand to treat arthritis. J Ethnopharmacol. 85:207–215. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Pongboonrod S: Medicinal Plants of East and Southeast Asia Attributed Properties and Use. Kasem Bunnakit Publishing; Bangkok: 1971

5 

Somchaichana J, Bunaprasert T and Patumraj S: Acanthus ebracteatus Vahl. ethanol extract enhancement of the efficacy of the collagen scaffold in wound closure: A study in a full-thickness- wound mouse model. J Biomed Biotechnol. 2012:7545272012. View Article : Google Scholar : PubMed/NCBI

6 

Mahasiripanth T, Hokputsa S, Niruthisard S, Bhattarakosol P and Patumraj S: Effects of Acanthus ebracteatus Vahl on tumor angiogenesis and on tumor growth in nude mice implanted with cervical cancer. Cancer Manag Res. 4:269–279. 2012.PubMed/NCBI

7 

WHO: World Health Statistics, . WHO Press; Geneva: 2018

8 

Chinembiri TN, du Plessis LH, Gerber M, Hamman JH and du Plessis J: Review of natural compounds for potential skin cancer treatment. Molecules. 19:11679–11721. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Sarkar FH, Li Y, Wang Z and Kong D: Cellular signaling perturbation by natural products. Cell Signal. 21:1541–1547. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Banjerdpongchai R, Wudtiwai B and Sringarm K: Cytotoxic and apoptotic-inducing effects of purple rice extracts and chemotherapeutic drugs on human cancer cell lines. Asian Pac J Cancer Prev. 14:6541–6548. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Cho JJ, Cho CL, Kao CL, Chen CM, Tseng CN, Lee YZ, Liao LJ and Hong YR: Crude aqueous extracts of Pluchea indica (L.) Less. Inhibit proliferation and migration of cancer cells through induction of p53-dependent cell death. BMC Complement Altern Med. 12:2652012. View Article : Google Scholar : PubMed/NCBI

12 

Sreelatha S, Jeyachitra A and Padma PR: Antiproliferation and induction of apoptosis by Moringa oleifera leaf extract on human cancer cells. Food Chem Toxicol. 49:1270–1275. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Nourazarian SM, Nourazarian A, Majidinia M and Roshaniasl E: Effect of root extracts of medicinal herb glycyrrhiza glabra on HSP90 gene expression and apoptosis in the HT-29 colon cancer cell line. Asian Pac J Cancer Prev. 16:8563–8566. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Shahidi F and Zhong Y: Bioactive peptides. J AOAC Int. 91:914–931. 2008.PubMed/NCBI

15 

Hou Y, Wu Z, Dai Z, Wang G and Wu G: Protein hydrolysates in animal nutrition: Industrial production, bioactive peptides, and functional significance. J Anim Sci Biotechnol. 8:242017. View Article : Google Scholar : PubMed/NCBI

16 

Marqus S, Pirogova E and Piva TJ: Evaluation of the use of therapeutic peptides for cancer treatment. J Biomed Sci. 24:212017. View Article : Google Scholar : PubMed/NCBI

17 

Bonifacino JS, Dasso M, Harford JB, Lippincott- Schwartz J and Yamada KM: Current Protocols in Cell Biology. John Wiley; New York: 1998

18 

Waiyaput W, Payungporn S, Issara-Amphorn J and Panjaworayan NT: Inhibitory effects of crude extracts from some edible Thai plants against replication of hepatitis B virus and human liver cancer cells. BMC Complement Altern Med. 12:2462012. View Article : Google Scholar : PubMed/NCBI

19 

Lear S and Cobb SL: Pep-Calc.com: A set of web utilities for the calculation of peptide and peptoid properties and automatic mass spectral peak assignment. J Comput Aided Mol Des. 30:271–277. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Schutte B, Nuydens R, Geerts H and Ramaekers F: Annexin V binding assay as a tool to measure apoptosis in differentiated neuronal cells. J Neurosci Methods. 86:63–69. 1998. View Article : Google Scholar : PubMed/NCBI

21 

Schmid I, Krall WJ, Uittenbogaart CH, Braun J and Giorgi JV: Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry. 13:204–208. 1992. View Article : Google Scholar : PubMed/NCBI

22 

Park DJ, Nakamura H, Chumakov AM, Said JW, Miller CW, Chen DL and Koeffler HP: Transactivational and DNA binding abilities of endogenous p53 in p53 mutant cell lines. Oncogene. 9:1899–1906. 1994.PubMed/NCBI

23 

Kwok TT, Mok CH and Menton-Brennan L: Up-regulation of a mutant form of p53 by doxorubicin in human squamous carcinoma cells. Cancer Res. 54:2834–2836. 1994.PubMed/NCBI

24 

Rivlin N, Koifman G and Rotter V: p53 orchestrates between normal differentiation and cancer. Semin Cancer Biol. 32:10–17. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Wong RP, Tsang WP, Chau PY, Co NN, Tsang TY and Kwok TT: p53-R273H gains new function in induction of drug resistance through down-regulation of procaspase-3. Mol Cancer Ther. 6:1054–1061. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Chen F, Castranova V and Shi X: New insights into the role of nuclear factor-kappaB in cell growth regulation. Am J Pathol. 159:387–397. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Alao JP: The regulation of cyclin D1 degradation: Roles in cancer development and the potential for therapeutic invention. Mol Cancer. 6:242007. View Article : Google Scholar : PubMed/NCBI

28 

Wang W, Takimoto R, Rastinejad F and El-Deiry WS: Stabilization of p53 by CP-31398 inhibits ubiquitination without altering phosphorylation at serine 15 or 20 or MDM2 binding. Mol Cell Biol. 23:2171–2181. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Pang LY, Saunders L and Argyle DJ: Epidermal growth factor receptor activity is elevated in glioma cancer stem cells and is required to maintain chemotherapy and radiation resistance. Oncotarget. 8:72494–72512. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Loughery J, Cox M, Smith LM and Meek DW: Critical role for p53-serine 15 phosphorylation in stimulating transactivation at p53-responsive promoters. Nucleic Acids Res. 42:7666–7680. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Hokputsa S, Harding SE, Inngjerdingen K, Jumel K, Michaelsen TE, Heinze T, Koschella A and Paulsen BS: Bioactive polysaccharides from the stems of the Thai medicinal plant Acanthus ebracteatus: Their chemical and physical features. Carbohydr Res. 339:753–762. 2004. View Article : Google Scholar : PubMed/NCBI

32 

Westerhoff HV, Zasloff M, Rosner JL, Hendler RW, De Waal A, Vaz Gomes A, Jongsma PM, Riethorst A and Juretić D: Functional synergism of the magainins PGLa and magainin-2 in Escherichia coli, tumor cells and liposomes. Eur J Biochem. 228:257–264. 1995. View Article : Google Scholar : PubMed/NCBI

33 

Hu C, Chen X, Huang Y and Chen Y: Synergistic effect of the pro-apoptosis peptide kla-TAT and the cationic anticancer peptide HPRP-A1. Apoptosis. 23:132–142. 2018. View Article : Google Scholar : PubMed/NCBI

34 

Casimiro MC, Crosariol M, Loro E, Li Z and Pestell RG: Cyclins and cell cycle control in cancer and disease. Genes Cancer. 3:649–657. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Prives C and Hall PA: The p53 pathway. J Pathol. 187:112–126. 1999. View Article : Google Scholar : PubMed/NCBI

36 

Sigal A and Rotter V: Oncogenic mutations of the p53 tumor suppressor: The demons of the guardian of the genome. Cancer Res. 60:6788–6793. 2000.PubMed/NCBI

37 

Tang X, Zhu Y, Han L, Kim AL, Kopelovich L, Bickers DR and Athar M: CP-31398 restores mutant p53 tumor suppressor function and inhibits UVB-induced skin carcinogenesis in mice. J Clin Invest. 117:3753–3764. 2007. View Article : Google Scholar : PubMed/NCBI

38 

Li D, Marchenko ND, Schulz R, Fischer V, Velasco-Hernandez T, Talos F and Moll UM: Functional inactivation of endogenous MDM2 and CHIP by HSP90 causes aberrant stabilization of mutant p53 in human cancer cells. Mol Cancer Res. 9:577–588. 2011. View Article : Google Scholar : PubMed/NCBI

39 

Brantley DM, Chen CL, Muraoka RS, Bushdid PB, Bradberry JL, Kittrell F, Medina D, Matrisian LM, Kerr LD and Yull FE: Nuclear factor-kappaB (NF-kappaB) regulates proliferation and branching in mouse mammary epithelium. Mol Biol Cell. 12:1445–1455. 2001. View Article : Google Scholar : PubMed/NCBI

40 

Hew CS, Khoo BY and Gam LH: The anti-cancer property of proteins extracted from Gynura procumbens (Lour.) Merr. PLoS One. 8:e685242013. View Article : Google Scholar : PubMed/NCBI

41 

Prateep A, Sumkhemthong S, Suksomtip M, Chanvorachote P and Chaotham C: Peptides extracted from edible mushroom: Lentinus squarrosulus induces apoptosis in human lung cancer cells. Pharm Biol. 55:1792–1799. 2017. View Article : Google Scholar : PubMed/NCBI

42 

Issara-Amphorn J and T-Thienprasert NP: Preliminary in vitro pro-apoptotic effects of Cratoxylum formosum crude leaf extracts. Int J Appl Res Nat Prod. 7:26–30. 2014.

43 

Budchart P, Khamwut A, Sinthuvanich C, Ratanapo S, Poovorawan Y and T-Thienprasert NP: Partially purified gloriosa superba peptides inhibit colon cancer cell viability by inducing apoptosis through p53 upregulation. Am J Med Sci. 354:423–429. 2017. View Article : Google Scholar : PubMed/NCBI

44 

Beg AA, Sha WC, Bronson RT, Ghosh S and Baltimore D: Embryonic lethality and liver degeneration in mice lacking the RelA component of NF-kappa B. Nature. 376:167–170. 1995. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Khamwut A, Jevapatarakul D, Reamtong O and T‑Thienprasert NP: In vitro evaluation of anti‑epidermoid cancer activity of Acanthus ebracteatus protein hydrolysate and their effects on apoptosis and cellular proteins. Oncol Lett 18: 3128-3136, 2019.
APA
Khamwut, A., Jevapatarakul, D., Reamtong, O., & T‑Thienprasert, N.P. (2019). In vitro evaluation of anti‑epidermoid cancer activity of Acanthus ebracteatus protein hydrolysate and their effects on apoptosis and cellular proteins. Oncology Letters, 18, 3128-3136. https://doi.org/10.3892/ol.2019.10647
MLA
Khamwut, A., Jevapatarakul, D., Reamtong, O., T‑Thienprasert, N. P."In vitro evaluation of anti‑epidermoid cancer activity of Acanthus ebracteatus protein hydrolysate and their effects on apoptosis and cellular proteins". Oncology Letters 18.3 (2019): 3128-3136.
Chicago
Khamwut, A., Jevapatarakul, D., Reamtong, O., T‑Thienprasert, N. P."In vitro evaluation of anti‑epidermoid cancer activity of Acanthus ebracteatus protein hydrolysate and their effects on apoptosis and cellular proteins". Oncology Letters 18, no. 3 (2019): 3128-3136. https://doi.org/10.3892/ol.2019.10647
Copy and paste a formatted citation
x
Spandidos Publications style
Khamwut A, Jevapatarakul D, Reamtong O and T‑Thienprasert NP: In vitro evaluation of anti‑epidermoid cancer activity of Acanthus ebracteatus protein hydrolysate and their effects on apoptosis and cellular proteins. Oncol Lett 18: 3128-3136, 2019.
APA
Khamwut, A., Jevapatarakul, D., Reamtong, O., & T‑Thienprasert, N.P. (2019). In vitro evaluation of anti‑epidermoid cancer activity of Acanthus ebracteatus protein hydrolysate and their effects on apoptosis and cellular proteins. Oncology Letters, 18, 3128-3136. https://doi.org/10.3892/ol.2019.10647
MLA
Khamwut, A., Jevapatarakul, D., Reamtong, O., T‑Thienprasert, N. P."In vitro evaluation of anti‑epidermoid cancer activity of Acanthus ebracteatus protein hydrolysate and their effects on apoptosis and cellular proteins". Oncology Letters 18.3 (2019): 3128-3136.
Chicago
Khamwut, A., Jevapatarakul, D., Reamtong, O., T‑Thienprasert, N. P."In vitro evaluation of anti‑epidermoid cancer activity of Acanthus ebracteatus protein hydrolysate and their effects on apoptosis and cellular proteins". Oncology Letters 18, no. 3 (2019): 3128-3136. https://doi.org/10.3892/ol.2019.10647
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