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Article

Multidrug resistance affects the prognosis of primary epithelial ovarian cancer

  • Authors:
    • Bo Gao
    • Fengmei Yang
    • Wei Chen
    • Rui Li
    • Xiuxue Hu
    • Yong Liang
    • Dongmin Li
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, Shanxi 710061, P.R. China, Department of Obstetrics and Gynecology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Laboratory Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Medical Office, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Anesthesiology, Ren‑ming Hospital of Yun‑xi, Shiyan, Hubei 442000, P.R. China
  • Pages: 4262-4269
    |
    Published online on: August 14, 2019
       https://doi.org/10.3892/ol.2019.10745
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Abstract

Multidrug‑resistant tumor cells can tolerate different structures, functions and antidrug action mechanisms, therefore, allowing these cells to respond to various structurally unrelated mechanisms of different chemotherapy drugs and to exhibit cross‑resistance. The present study aimed to investigate the role of Multi‑drug resistance gene (MDR1), Placental glutathione S‑transferase‑P1 (GSTP1), Lung resistance protein (LRP) and Ras association domain family member 1 (RASSF1A) in primary epithelial ovarian cancer (PEOC). The mRNA (protein) expression levels of MDR1, product P glycoprotein, LRP and GSTP1 were evaluated with reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blot analysis in all tissue samples, ovarian cancer cell line A2780 and A2780/DDP. Methylation‑specific PCR (MSP) was used to detect RASSF1A gene methylation in all tissue samples. The resistance genes/proteins were either poorly or not expressed in A2780, however were highly expressed in A2780/DDP cell line. The expression of resistance genes/proteins decreased following different concentrations of zebularine‑stimulated A2780/DDP. Hypermethylation and low expression of RASSF1A gene were detected in PEOC and A2780/DDP. Subsequent to being exposed to different concentrations of zebularine‑stimulated A2780/DDP, the RASSF1A methylation level was decreased, while the unmethylation level was increased. The expression of RASSF1A gene/protein was gradually restored, and the gene/protein expression was enhanced with the increase in drug concentration. Multivariate logistic regression indicated that the expression level of gene LRP and GSTP1 was a risk factor for PEOC prognosis. Furthermore, the expression of LRP and GSTP1 in the negative‑group survival curves was higher compared with the positive group. High expression of resistance genes may serve an important role in cancer primary resistance. Low expression caused by hyper‑methylation of RASSF1A gene may serve an important role in cancer‑acquired resistance in PEOC. The present study suggested that resistant gene expression may be a potential prognostic biomarker.
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Copy and paste a formatted citation
Spandidos Publications style
Gao B, Yang F, Chen W, Li R, Hu X, Liang Y and Li D: Multidrug resistance affects the prognosis of primary epithelial ovarian cancer. Oncol Lett 18: 4262-4269, 2019.
APA
Gao, B., Yang, F., Chen, W., Li, R., Hu, X., Liang, Y., & Li, D. (2019). Multidrug resistance affects the prognosis of primary epithelial ovarian cancer. Oncology Letters, 18, 4262-4269. https://doi.org/10.3892/ol.2019.10745
MLA
Gao, B., Yang, F., Chen, W., Li, R., Hu, X., Liang, Y., Li, D."Multidrug resistance affects the prognosis of primary epithelial ovarian cancer". Oncology Letters 18.4 (2019): 4262-4269.
Chicago
Gao, B., Yang, F., Chen, W., Li, R., Hu, X., Liang, Y., Li, D."Multidrug resistance affects the prognosis of primary epithelial ovarian cancer". Oncology Letters 18, no. 4 (2019): 4262-4269. https://doi.org/10.3892/ol.2019.10745
Copy and paste a formatted citation
x
Spandidos Publications style
Gao B, Yang F, Chen W, Li R, Hu X, Liang Y and Li D: Multidrug resistance affects the prognosis of primary epithelial ovarian cancer. Oncol Lett 18: 4262-4269, 2019.
APA
Gao, B., Yang, F., Chen, W., Li, R., Hu, X., Liang, Y., & Li, D. (2019). Multidrug resistance affects the prognosis of primary epithelial ovarian cancer. Oncology Letters, 18, 4262-4269. https://doi.org/10.3892/ol.2019.10745
MLA
Gao, B., Yang, F., Chen, W., Li, R., Hu, X., Liang, Y., Li, D."Multidrug resistance affects the prognosis of primary epithelial ovarian cancer". Oncology Letters 18.4 (2019): 4262-4269.
Chicago
Gao, B., Yang, F., Chen, W., Li, R., Hu, X., Liang, Y., Li, D."Multidrug resistance affects the prognosis of primary epithelial ovarian cancer". Oncology Letters 18, no. 4 (2019): 4262-4269. https://doi.org/10.3892/ol.2019.10745
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