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Mechanism of miR‑107‑targeting of regulator of G‑protein signaling 4 in hepatocellular carcinoma

  • Authors:
    • Di Xiao
    • Hai‑Xia Gao
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    Affiliations: Department of Liver Disease, Jinan Infectious Disease Hospital, Jinan, Shandong 250021, P.R. China, Department of ICU, Jinan Infectious Disease Hospital, Jinan, Shandong 250021, P.R. China
    Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5145-5154
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    Published online on: September 12, 2019
       https://doi.org/10.3892/ol.2019.10857
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Abstract

The aim of the present study was to investigate the mechanism of microRNA (miR)‑107 in targeting regulator of G‑protein signaling 4 (RGS4) in hepatic carcinoma. SK‑HEP‑1 cells were transfected with miR‑107 mimics and control mimics. Reverse transcription‑quantitative PCR was performed to determine the miR‑107 expression levels, and following miR‑107 upregulation, MTT, colony formation, transwell and wound‑healing assays were performed to assess cell proliferation, colony‑forming ability, invasion and migration, respectively. In addition, the effect of miR‑107 upregulation on the cell cycle and apoptosis in SK‑HEP‑1 cells was evaluated using flow cytometry. Western blot analysis was performed to measure the protein expression levels of RGS4, epidermal growth factor receptor (EGFR), CXC chemokine receptor type 4 (CXCR4) and matrix metalloproteinase (MMP)‑2 and ‑9. Expression level changes and the association between miR‑107 and RGS4 in HCC cells were assessed using dual luciferase analysis. The results indicated that the overexpression of miR‑107 in HCC cells suppressed cellular proliferation, invasion, migration and colony‑forming ability, but promoted apoptosis and G1 phase arrest. Furthermore, miR‑107 mimics notably increased the protein expression level of RGS4, but significantly downregulated that of EGFR, CXCR4 and MMP‑2 and ‑9. Together, these findings suggest that targeting this potential mechanism of miR‑107 may be beneficial in the treatment of patients with HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Xiao D and Gao HX: Mechanism of miR‑107‑targeting of regulator of G‑protein signaling 4 in hepatocellular carcinoma. Oncol Lett 18: 5145-5154, 2019.
APA
Xiao, D., & Gao, H. (2019). Mechanism of miR‑107‑targeting of regulator of G‑protein signaling 4 in hepatocellular carcinoma. Oncology Letters, 18, 5145-5154. https://doi.org/10.3892/ol.2019.10857
MLA
Xiao, D., Gao, H."Mechanism of miR‑107‑targeting of regulator of G‑protein signaling 4 in hepatocellular carcinoma". Oncology Letters 18.5 (2019): 5145-5154.
Chicago
Xiao, D., Gao, H."Mechanism of miR‑107‑targeting of regulator of G‑protein signaling 4 in hepatocellular carcinoma". Oncology Letters 18, no. 5 (2019): 5145-5154. https://doi.org/10.3892/ol.2019.10857
Copy and paste a formatted citation
x
Spandidos Publications style
Xiao D and Gao HX: Mechanism of miR‑107‑targeting of regulator of G‑protein signaling 4 in hepatocellular carcinoma. Oncol Lett 18: 5145-5154, 2019.
APA
Xiao, D., & Gao, H. (2019). Mechanism of miR‑107‑targeting of regulator of G‑protein signaling 4 in hepatocellular carcinoma. Oncology Letters, 18, 5145-5154. https://doi.org/10.3892/ol.2019.10857
MLA
Xiao, D., Gao, H."Mechanism of miR‑107‑targeting of regulator of G‑protein signaling 4 in hepatocellular carcinoma". Oncology Letters 18.5 (2019): 5145-5154.
Chicago
Xiao, D., Gao, H."Mechanism of miR‑107‑targeting of regulator of G‑protein signaling 4 in hepatocellular carcinoma". Oncology Letters 18, no. 5 (2019): 5145-5154. https://doi.org/10.3892/ol.2019.10857
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