SNX3 suppresses the migration and invasion of colorectal cancer cells by reversing epithelial‑to‑mesenchymal transition via the β‑catenin pathway

Pan,Biran; Zhang,Tongtong; Yang,Wei; Liu,Yanjun; Chen,Yuning; Zhou,Zheng; Tang,Yan; Zeng,Jiawei; Liu,Yilun; Zhao,Cong; Guo,Yuanbiao

doi:10.3892/ol.2019.10860

SNX3 suppresses the migration and invasion of colorectal cancer cells by reversing epithelial‑to‑mesenchymal transition via the β‑catenin pathway

Authors:
- Biran Pan
- Tongtong Zhang
- Wei Yang
- Yanjun Liu
- Yuning Chen
- Zheng Zhou
- Yan Tang
- Jiawei Zeng
- Yilun Liu
- Cong Zhao
- Yuanbiao Guo
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Affiliations: Medical Research Center, The Third People's Hospital of Chengdu, The Second Chengdu Hospital Affiliated to Chongqing Medical University, The Affiliated Hospital of Southwest Jiaotong University, Chengdu, Sichuan 610036, P.R. China, Department of Gastrointestinal Surgery, The Third People's Hospital of Chengdu, The Second Chengdu Hospital Affiliated to Chongqing Medical University, The Affiliated Hospital of Southwest Jiaotong University, Chengdu, Sichuan 610036, P.R. China, Clinical Laboratory Department, Xindu People's Hospital, Chengdu, Sichuan 610500, P.R. China, Department of Pathology, The Third People's Hospital of Chengdu, The Second Chengdu Hospital Affiliated to Chongqing Medical University, The Affiliated Hospital of Southwest Jiaotong University, Chengdu, Sichuan 610036, P.R. China , Clinical Laboratory Department, Mianyang Central Hospital, Mianyang, Sichuan 621000, P.R. China , Clinical Laboratory Department, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China , Department of Digestive Diseases, The Third People's Hospital of Chengdu, The Second Chengdu Hospital Affiliated to Chongqing Medical University, The Affiliated Hospital of Southwest Jiaotong University, Chengdu, Sichuan 610036, P.R. China
Published online on: September 12, 2019 https://doi.org/10.3892/ol.2019.10860
Pages: 5332-5340
Copyright: © Pan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The Wnt/β‑catenin signaling pathway is a well‑studied pathway that drives the carcinogenesis and metastasis of colorectal cancer (CRC). The secretion of Wnt proteins is essential for the continuous activation of Wnt/β‑catenin signaling in CRC. The secretion of Drosophila wingless, which is homologous to the human Wnt protein, is mediated by sorting nexin 3 (SNX3) in Drosophila; however, the role of SNX3 in CRC remains unknown. In the present study it was demonstrated that SNX3 reduced the migratory and invasive ability of HCT116 human CRC cells, and reversed epithelial‑mesenchymal transition (EMT). Conversely, in the HT29 CRC cell line, which endogenously expresses high levels of SNX3, short hairpin RNA or siRNA‑mediated knockdown of SNX3 induced EMT, and enhanced cell migration and invasion. In addition, upregulation of SNX3 significantly inhibited metastasis of HCT116 cells to the lungs of mice. These SNX3‑mediated effects were associated with downregulation of β‑catenin. Taken together, by downregulating β‑catenin, SNX3 may mediate EMT and reverse CRC metastasis.

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