Identification and validation of microRNAs and their targets expressed in osteosarcoma

  • Authors:
    • Can Zhang
    • Jun Wan
    • Feng Long
    • Qing Liu
    • Hongbo He
  • View Affiliations

  • Published online on: September 13, 2019     https://doi.org/10.3892/ol.2019.10864
  • Pages: 5628-5636
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Osteosarcoma (OS) is the most common type of bone cancer in children and adolescents, and has a poor prognosis. Previous studies have demonstrated that a number of microRNAs (miRNAs) were deregulated in OS, and that the expression of certain miRNAs was correlated with the stage of OS. Therefore, miRNAs may serve a role as a diagnostic and prognostic biomarker of OS. miRNA and mRNA integrated analysis of public expression profiles in the Gene Expression Omnibus database for OS was performed, and the regulated targets of miRNA in OS were predicted. Next, the regulatory network of miRNAs/genes was constructed and verified by reverse transcription‑quantitative polymerase chain reaction in tissues and MG‑63 cell lines. Two miRNA expression profiling studies and four eligible mRNA expression profiling studies were selected. Ten upregulated miRNAs, 5 downregulated miRNAs and 5 DGEs were identified in OS compared with normal tissues. hsa‑miR‑346 was inversely correlated with the target gene c‑FLIP, which was consistent with the results of integrated analysis. In vitro, pre‑miRNA‑346 can downregulate the protein expression of c‑FLIP, while not changing the mRNA level of c‑FLIP. In the regulatory network, hsa‑miR‑346 and its target gene, c‑FLIP, can be used as biomarkers for an earlier diagnosis of OS.
View Figures
View References

Related Articles

Journal Cover

November-2019
Volume 18 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang C, Wan J, Long F, Liu Q and He H: Identification and validation of microRNAs and their targets expressed in osteosarcoma. Oncol Lett 18: 5628-5636, 2019
APA
Zhang, C., Wan, J., Long, F., Liu, Q., & He, H. (2019). Identification and validation of microRNAs and their targets expressed in osteosarcoma. Oncology Letters, 18, 5628-5636. https://doi.org/10.3892/ol.2019.10864
MLA
Zhang, C., Wan, J., Long, F., Liu, Q., He, H."Identification and validation of microRNAs and their targets expressed in osteosarcoma". Oncology Letters 18.5 (2019): 5628-5636.
Chicago
Zhang, C., Wan, J., Long, F., Liu, Q., He, H."Identification and validation of microRNAs and their targets expressed in osteosarcoma". Oncology Letters 18, no. 5 (2019): 5628-5636. https://doi.org/10.3892/ol.2019.10864