Involvement of the TGF-β1 pathway in caveolin-1-associated regulation of head and neck tumor cell metastasis

Sun,Jinjie; Lu,Yongtian; Yu,Changyun; Xu,Ting; Nie,Guohui; Miao,Beiping; Zhang,Xin

doi:10.3892/ol.2019.11187

Involvement of the TGF-β1 pathway in caveolin-1-associated regulation of head and neck tumor cell metastasis

Authors:
- Jinjie Sun
- Yongtian Lu
- Changyun Yu
- Ting Xu
- Guohui Nie
- Beiping Miao
- Xin Zhang
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Affiliations: Department of Otolaryngology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China, Department of Otolaryngology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
Published online on: December 6, 2019 https://doi.org/10.3892/ol.2019.11187
Pages: 1298-1304
Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Head and neck squamous cell carcinoma (HNSCC) is the sixth most frequent malignancy with a 5-year survival rate of 54%. Therefore, disease management improvement is required. The present study aimed to assess the role of caveolin-1 (Cav-1) in the metastasis of head and neck tumor cells. Short hairpin RNA was used to silence Cav-1 expression in Tu686 cells. Proliferation, migration, invasion, morphology and the levels of effector proteins were assessed in cells. Upon Cav-1 silencing, E-cadherin levels were decreased, while vimentin levels were significantly increased. Cell migration, quantified by wound healing and Transwell assays, was significantly increased. Meanwhile, Cav-1 and transforming growth factor β1 (TGF-β1) receptor were identified to be co-localized. In addition, Cav-1-knockdown resulted in increased phosphorylation of SMAD family member 2 (P<0.05), a downstream effector of TGF-β signaling. In addition, there was a mutual regulation, with increasing TGF-β1 levels leading to a dose‑dependent decrease of Cav-1 expression levels (P<0.05). These findings indicate that Cav-1 inhibits cell metastasis in HNSCC, suggesting the involvement of the TGF-β signaling pathway.

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