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Integrated bioinformatics analysis to screen hub genes in the lymph node metastasis of thyroid cancer

  • Authors:
    • Si Lu
    • Rongjie Zhao
    • Jie Shen
    • Yu Zhang
    • Jingjing Shi
    • Chenke Xu
    • Jiali Chen
    • Renbin Lin
    • Weidong Han
    • Dingcun Luo
  • View Affiliations / Copyright

    Affiliations: Zhejiang Chinese Medical University Affiliated Hangzhou First Hospital, The Fourth Clinical College, Hangzhou First People's Hospital, Hangzhou, Zhejiang 310006, P.R. China, Department of Medical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310000, P.R. China, Nanjing Medical University Affiliated Hangzhou Hospital, The First Clinical College, Hangzhou First People's Hospital, Hangzhou, Zhejiang 310006, P.R. China, Department of Oncological Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, P.R. China, Department of Ultrasound, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, P.R. China, Department of Gastroenterology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310000, P.R. China
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1375-1383
    |
    Published online on: December 6, 2019
       https://doi.org/10.3892/ol.2019.11188
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Abstract

Thyroid cancer (TC) is one of the most common types of malignancy of the endocrine‑system. At present, there is a lack of effective methods to predict neck lymph node metastasis (LNM) in TC. The present study compared the expression profiles from The Cancer Genome Atlas between N1M0 and N0M0 subgroups in each T1‑4 stages TC in order to identify the four groups of TC LNM‑associated differentially expressed genes (DEGs). Subsequently, DEGs were combined to obtain a total of 493 integrated DEGs by using the method of Robust Rank Aggregation. Furthermore, the underlying mechanisms of LNM were investigated. The results from Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses demonstrated that the identified DEGs may promote LNM via numerous pathways, including extracellular matrix‑receptor interaction, PI3K‑AKT signaling pathway and focal adhesion. Following construction of a protein‑protein interaction network, the significance score for each gene was calculated and seven hub genes were screened, including interleukin 6, actinin α2, collagen type I α 1 chain, actin α1, calbindin 2, thrombospondin 1 and parathyroid hormone. These genes were predicted to serve crucial roles in TC with LNM. The results from the present study could therefore improve the understanding of LNM in TC. In addition, the seven DEGs identified may be considered as potential novel targets for the development of biomarkers that could be used in the diagnosis and therapy of TC.
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Copy and paste a formatted citation
Spandidos Publications style
Lu S, Zhao R, Shen J, Zhang Y, Shi J, Xu C, Chen J, Lin R, Han W, Luo D, Luo D, et al: Integrated bioinformatics analysis to screen hub genes in the lymph node metastasis of thyroid cancer. Oncol Lett 19: 1375-1383, 2020.
APA
Lu, S., Zhao, R., Shen, J., Zhang, Y., Shi, J., Xu, C. ... Luo, D. (2020). Integrated bioinformatics analysis to screen hub genes in the lymph node metastasis of thyroid cancer. Oncology Letters, 19, 1375-1383. https://doi.org/10.3892/ol.2019.11188
MLA
Lu, S., Zhao, R., Shen, J., Zhang, Y., Shi, J., Xu, C., Chen, J., Lin, R., Han, W., Luo, D."Integrated bioinformatics analysis to screen hub genes in the lymph node metastasis of thyroid cancer". Oncology Letters 19.2 (2020): 1375-1383.
Chicago
Lu, S., Zhao, R., Shen, J., Zhang, Y., Shi, J., Xu, C., Chen, J., Lin, R., Han, W., Luo, D."Integrated bioinformatics analysis to screen hub genes in the lymph node metastasis of thyroid cancer". Oncology Letters 19, no. 2 (2020): 1375-1383. https://doi.org/10.3892/ol.2019.11188
Copy and paste a formatted citation
x
Spandidos Publications style
Lu S, Zhao R, Shen J, Zhang Y, Shi J, Xu C, Chen J, Lin R, Han W, Luo D, Luo D, et al: Integrated bioinformatics analysis to screen hub genes in the lymph node metastasis of thyroid cancer. Oncol Lett 19: 1375-1383, 2020.
APA
Lu, S., Zhao, R., Shen, J., Zhang, Y., Shi, J., Xu, C. ... Luo, D. (2020). Integrated bioinformatics analysis to screen hub genes in the lymph node metastasis of thyroid cancer. Oncology Letters, 19, 1375-1383. https://doi.org/10.3892/ol.2019.11188
MLA
Lu, S., Zhao, R., Shen, J., Zhang, Y., Shi, J., Xu, C., Chen, J., Lin, R., Han, W., Luo, D."Integrated bioinformatics analysis to screen hub genes in the lymph node metastasis of thyroid cancer". Oncology Letters 19.2 (2020): 1375-1383.
Chicago
Lu, S., Zhao, R., Shen, J., Zhang, Y., Shi, J., Xu, C., Chen, J., Lin, R., Han, W., Luo, D."Integrated bioinformatics analysis to screen hub genes in the lymph node metastasis of thyroid cancer". Oncology Letters 19, no. 2 (2020): 1375-1383. https://doi.org/10.3892/ol.2019.11188
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