Open Access

Inhibition mechanism of lung cancer cell metastasis through targeted regulation of Smad3 by miR‑15a

  • Authors:
    • Shuai Guo
    • Ming Li
    • Juan Li
    • Yan Lv
  • View Affiliations

  • Published online on: December 10, 2019     https://doi.org/10.3892/ol.2019.11194
  • Pages: 1516-1522
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Effect of targeted regulation of mothers against decapentaplegic homolog 3 (Smad3) by microRNA-15a (miR‑15a) on the proliferation, invasion and metastasis of non‑small cell lung cancer (NSCLC) cells and its related mechanisms were investigated. Fifty pairs of NSCLC and para‑cancerous tissues were collected to identify the expression level of miR‑15a in NSCLC, para‑cancerous tissue, and cell lines A549, H1299, H1975 and BEAS‑2B by real‑time fluorescence quantitative PCR (RT‑PCR); A549 cells were transfected with miR‑15a mimic; the MTT assay was performed to detect the role of miR‑15a transfection in proliferation of A549 cells, the wound healing assay was carried out to identify the role of miR‑15a in migration of A549 cells; Transwell invasion assay was conducted to analyze the role of miR‑15a in invasion of A549 cells; western blotting was carried out to find the effect of miR‑15a on Smad3 expression, and Spearman's rank correlation was used to analyze the correlation between miR‑15a and Smad3 expression. NSCLC tissues and cells showed significantly lower miR‑15a expression, compared with para‑cancerous tissues and normal cell lines (P=0.023). miR‑15a was significantly more expressed in A549 cells transfected with miR‑15a mimic (P=0.043). Overexpression of miR‑15a can significantly inhibit A549 cell proliferation (P=0.038), migration (P=0.033) and invasion (P=0.025), and significantly reduced the expression level of Smad3 (P=0.031) in A549 cells. Spearman's rank correlation showed negative correlation of miR‑15a expression with Smad3, which may indicate negative regulation (r=‑0.34, P<0.0001). Inhibition of proliferation, migration and invasion of NSCLC cells can be achieved with targeted regulation of Smad3 by miR‑15a.
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February-2020
Volume 19 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Guo S, Li M, Li J and Lv Y: Inhibition mechanism of lung cancer cell metastasis through targeted regulation of Smad3 by miR‑15a. Oncol Lett 19: 1516-1522, 2020.
APA
Guo, S., Li, M., Li, J., & Lv, Y. (2020). Inhibition mechanism of lung cancer cell metastasis through targeted regulation of Smad3 by miR‑15a. Oncology Letters, 19, 1516-1522. https://doi.org/10.3892/ol.2019.11194
MLA
Guo, S., Li, M., Li, J., Lv, Y."Inhibition mechanism of lung cancer cell metastasis through targeted regulation of Smad3 by miR‑15a". Oncology Letters 19.2 (2020): 1516-1522.
Chicago
Guo, S., Li, M., Li, J., Lv, Y."Inhibition mechanism of lung cancer cell metastasis through targeted regulation of Smad3 by miR‑15a". Oncology Letters 19, no. 2 (2020): 1516-1522. https://doi.org/10.3892/ol.2019.11194