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Effects of miR‑129‑3p on biological functions of prostate cancer cells through targeted regulation of Smad3

  • Authors:
    • Yunpeng Jia
    • Yu Gao
    • Jianguo Dou
  • View Affiliations / Copyright

    Affiliations: Department of Urology Surgery, Gansu Provincial Hospital of TCM, Lanzhou, Gansu 730050, P.R. China, Department of Urology Surgery, The Dazu District People's Hospital, Chongqing 402360, P.R. China
    Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1195-1202
    |
    Published online on: December 13, 2019
       https://doi.org/10.3892/ol.2019.11216
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Abstract

Effects of miR‑129‑3p on the biological functions of prostate cancer cells through the targeted regulation of Smad3 were investigated. RT‑PCR was used to detect the expression levels of miR‑129‑3p in prostate cancer tissues and cells and its target gene Smad3 mRNA determined by bioinformatics prediction. Correlation between miR‑129‑3p and Smad3 was analyzed. MTT assay, cell invasion detection, and apoptosis detection were conducted to detect the effects of miR‑129‑3p and Smad3 on the proliferation, invasion, and apoptosis of prostate cancer cells. The results of RT‑qPCR showed that the expression level of miR‑129‑3p decreased but that of Smad3 increased in the prostate cancer tissue, and the expression levels of the two were significantly and negatively correlated. Additionally, the expression levels were closely related to the degree of tumor differentiation, TNM staging, and lymph node metastasis (P<0.05). Bioinformatics prediction and subsequent experiments proved that Smad3 was the direct target gene of miR‑129‑3p. Cell detection confirmed that the overexpression of miR‑129‑3p or the inhibition of Smad3 expression inhibited the proliferation and invasion of prostate cancer cells, promoting apoptosis, and increased the expression level of pro‑apoptotic protein Bax, as well as decreased the expression level of anti‑apoptotic protein Bcl‑2. Inhibition of miR‑129‑3p expression had the opposite effect to overexpression. miR‑129‑3p, which may be a new and potential target for the treatment of prostate cancer, can inhibit the proliferation and invasion of prostate cancer cells and promote their apoptosis by directly targeting Smad3.
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1 

Fizazi K, Tran N, Fein L, Matsubara N, Rodriguez-Antolin A, Alekseev BY, Özgüroğlu M, Ye D, Feyerabend S, Protheroe A, et al: Abiraterone plus Prednisone in metastatic, castration-sensitive prostate cancer. Lancet Oncol. 20:686–700. 2019. View Article : Google Scholar : PubMed/NCBI

2 

Gillessen S, Attard G, Beer TM, Beltran H, Bossi A, Bristow R, Carver B, Castellano D, Chung BH, Clarke N, et al: Management of patients with advanced prostate cancer: The report of the Advanced Prostate Cancer Consensus Conference APCCC 2017. Eur Urol. 73:178–211. 2018. View Article : Google Scholar : PubMed/NCBI

3 

Madaan S, Abel PD, Chaudhary KS, Hewitt R, Stott MA, Stamp GW and Lalani EN: Cytoplasmic induction and over-expression of cyclooxygenase-2 in human prostate cancer: Implications for prevention and treatment. BJU Int. 86:736–741. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Hao NB, He YF, Li XQ, Wang K and Wang RL: The role of miRNA and lncRNA in gastric cancer. Oncotarget. 8:81572–81582. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Fang H, Xie J, Zhang M, Zhao Z, Wan Y and Yao Y: miRNA-21 promotes proliferation and invasion of triple-negative breast cancer cells through targeting PTEN. Am J Transl Res. 9:953–961. 2017.PubMed/NCBI

6 

Liu K, Huang J, Ni J, Song D, Ding M, Wang J, Huang X and Li W: MALAT1 promotes osteosarcoma development by regulation of HMGB1 via miR-142-3p and miR-129-5p. Cell Cycle. 16:578–587. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Lin VC, Huang SP, Ting HJ, Ma WL, Yu CC, Huang CY, Yin HL, Huang TY, Lee CH, Chang TY, et al: Vitamin D receptor-binding site variants affect prostate cancer progression. Oncotarget. 8:74119–74128. 2017.PubMed/NCBI

8 

Lu C, Shan Z, Li C and Yang L: miR-129 regulates cisplatin- resistance in human gastric cancer cells by targeting P-gp. Biomed Pharmacother. 86:450–456. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Zuo Y, Li Y, Zhou Z, Ma M and Fu K: Long non-coding RNA MALAT1 promotes proliferation and invasion via targeting miR-129-5p in triple-negative breast cancer. Biomed Pharmacother. 95:922–928. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Tang PM, Zhou S, Meng XM, Wang QM, Li CJ, Lian GY, Huang XR, Tang YJ, Guan XY, Yan BP, et al: Smad3 promotes cancer progression by inhibiting E4BP4-mediated NK cell development. Nat Commun. 8:146772017. View Article : Google Scholar : PubMed/NCBI

11 

Pashaei E, Pashaei E, Ahmady M, Ozen M and Aydin N: Meta-analysis of miRNA expression profiles for prostate cancer recurrence following radical prostatectomy. PLoS One. 12:e01795432017. View Article : Google Scholar : PubMed/NCBI

12 

Lu S, Wang MS, Chen PJ, Ren Q and Bai P: miRNA-186 inhibits prostate cancer cell proliferation and tumor growth by targeting YY1 and CDK6. Exp Ther Med. 13:3309–3314. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Liu C, Liu R, Zhang D, Deng Q, Liu B, Chao HP, Rycaj K, Takata Y, Lin K, Lu Y, et al: MicroRNA-141 suppresses prostate cancer stem cells and metastasis by targeting a cohort of pro-metastasis genes. Nat Commun. 8:142702017. View Article : Google Scholar : PubMed/NCBI

14 

Varambally S, Cao Q, Mani RS, Shankar S, Wang X, Ateeq B, Laxman B, Cao X, Jing X, Ramnarayanan K, et al: Genomic loss of microRNA-101 leads to overexpression of histone methyltransferase EZH2 in cancer. Science. 322:1695–1699. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Elzein S and Goodyer CG: Regulation of human growth hormone receptor expression by microRNAs. Mol Endocrinol. 28:1448–1459. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Bijnsdorp IV, Hodzic J, Lagerweij T, Westerman B, Krijgsman O, Broeke J, Verweij F, Nilsson RJ, Rozendaal L, van Beusechem VW, et al: miR-129-3p controls centrosome number in metastatic prostate cancer cells by repressing CP110. Oncotarget. 7:16676–16687. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Huang S, Liao Q, Li L and Xin D: PTTG1 inhibits SMAD3 in prostate cancer cells to promote their proliferation. Tumour Biol. 35:6265–6270. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Majumder S, Bhowal A, Basu S, Mukherjee P, Chatterji U and Sengupta S: Deregulated E2F5/p38/SMAD3 circuitry reinforces the pro-tumorigenic switch of TGFβ signaling in prostate cancer. J Cell Physiol. 231:2482–2492. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Yao B, Zhao J, Li Y, Li H, Hu Z, Pan P, Zhang Y, Du E, Liu R and Xu Y: Elf5 inhibits TGF-β-driven epithelial-mesenchymal transition in prostate cancer by repressing SMAD3 activation. Prostate. 75:872–882. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Lu S, Lee J, Revelo M, Wang X, Lu S and Dong Z: Smad3 is overexpressed in advanced human prostate cancer and necessary for progressive growth of prostate cancer cells in nude mice. Clin Cancer Res. 13:5692–5702. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Virgo KS, Rumble RB and Singer EA: Second-line hormonal therapy for men with chemotherapy-naïve castration-resistant prostate cancer: American Society of Clinical Oncology Provisional Clinical Opinion Summary. J Oncol Pract. 13:459–461. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Teixeira AL, Gomes M, Nogueira A, Azevedo AS, Assis J, Dias F, Santos JI, Lobo F, Morais A, Maurício J, et al: Improvement of a predictive model of castration-resistant prostate cancer: Functional genetic variants in TGFβ1 signaling pathway modulation. PLoS One. 8:e72419. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Song K, Wang H, Krebs TL, Wang B, Kelley TJ and Danielpour D: DHT selectively reverses Smad3-mediated/TGF-beta-induced responses through transcriptional down-regulation of Smad3 in prostate epithelial cells. Mol Endocrinol. 24:2019–2029. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Ma L, Chen X, Li C, Cheng R, Gao Z, Meng X, Sun C, Liang C and Liu Y: miR-129-5p and −3p co-target WWP1 to suppress gastric cancer proliferation and migration. J Cell Biochem. Nov 11–2018.(Epub ahead of print). doi: 10.1002/jcb.28027.

25 

Fang D-Z, Wang Y-P, Liu J, Hui XB, Wang XD, Chen X and Liu D: MicroRNA-129-3p suppresses tumor growth by targeting E2F5 in glioblastoma. Eur Rev Med Pharmacol Sci. 22:1044–1050. 2018.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jia Y, Gao Y and Dou J: Effects of miR‑129‑3p on biological functions of prostate cancer cells through targeted regulation of Smad3. Oncol Lett 19: 1195-1202, 2020.
APA
Jia, Y., Gao, Y., & Dou, J. (2020). Effects of miR‑129‑3p on biological functions of prostate cancer cells through targeted regulation of Smad3. Oncology Letters, 19, 1195-1202. https://doi.org/10.3892/ol.2019.11216
MLA
Jia, Y., Gao, Y., Dou, J."Effects of miR‑129‑3p on biological functions of prostate cancer cells through targeted regulation of Smad3". Oncology Letters 19.2 (2020): 1195-1202.
Chicago
Jia, Y., Gao, Y., Dou, J."Effects of miR‑129‑3p on biological functions of prostate cancer cells through targeted regulation of Smad3". Oncology Letters 19, no. 2 (2020): 1195-1202. https://doi.org/10.3892/ol.2019.11216
Copy and paste a formatted citation
x
Spandidos Publications style
Jia Y, Gao Y and Dou J: Effects of miR‑129‑3p on biological functions of prostate cancer cells through targeted regulation of Smad3. Oncol Lett 19: 1195-1202, 2020.
APA
Jia, Y., Gao, Y., & Dou, J. (2020). Effects of miR‑129‑3p on biological functions of prostate cancer cells through targeted regulation of Smad3. Oncology Letters, 19, 1195-1202. https://doi.org/10.3892/ol.2019.11216
MLA
Jia, Y., Gao, Y., Dou, J."Effects of miR‑129‑3p on biological functions of prostate cancer cells through targeted regulation of Smad3". Oncology Letters 19.2 (2020): 1195-1202.
Chicago
Jia, Y., Gao, Y., Dou, J."Effects of miR‑129‑3p on biological functions of prostate cancer cells through targeted regulation of Smad3". Oncology Letters 19, no. 2 (2020): 1195-1202. https://doi.org/10.3892/ol.2019.11216
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