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Article

miR‑519 regulates the proliferation of breast cancer cells via targeting human antigen R

  • Authors:
    • Lili Ren
    • Yong Li
    • Qun Zhao
    • Liqiao Fan
    • Bibo Tan
    • Aimin Zang
    • Hua Yang
  • View Affiliations / Copyright

    Affiliations: Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Department of Medical Oncology, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China
  • Pages: 1567-1576
    |
    Published online on: December 20, 2019
       https://doi.org/10.3892/ol.2019.11230
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Abstract

Breast cancer is one of the most prevalent types of cancer among women that leads to millions of deaths worldwide every year. The mechanisms of breast cancer pathogenesis remain unclear. It has been reported that aberrant expression of miR‑519, is associated with breast cancer development; however, the effects of miR‑519 on breast cancer cell proliferation remain unknown. Therefore, the present study aimed to determine whether miR‑519 could regulate breast cancer cell proliferation. A total of 20 pairs of primary breast cancer and adjacent normal tissues were collected from patients with breast cancer. miR‑519 expression level was determined by reverse transcription‑quantitative polymerase chain reaction. Furthermore, miR‑519 mimics or inhibitors were transfected into breast cancer MCF‑7 cells in order to up‑ or downregulate miR‑519 expression. Subsequently, human antigen R (HUR), BCL‑2 and BAX protein levels were analyzed by western blotting. MCF‑7 cell proliferation was assessed using MTT and colony formation assays. A luciferase assay was performed to verify whether miR‑519 could directly bind to HUR mRNA. The results demonstrated that miR‑519 expression level was lower in primary breast cancer tissues compared with adjacent normal tissues. Furthermore, miR‑519 overexpression and downregulation inhibited and stimulated MCF‑7 cell proliferation, respectively. In addition, the results from luciferase assay demonstrated that HUR was a target of miR‑519. HUR overexpression could reverse the effect of miR‑519 mimics on MCF‑7 cell proliferation, whereas HUR silencing could rescue the effect of miR‑519 inhibitors on MCF‑7 cell proliferation. These findings suggested that miR‑519 may regulate MCF‑7 cell proliferation by targeting HUR.
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Copy and paste a formatted citation
Spandidos Publications style
Ren L, Li Y, Zhao Q, Fan L, Tan B, Zang A and Yang H: miR‑519 regulates the proliferation of breast cancer cells via targeting human antigen R. Oncol Lett 19: 1567-1576, 2020.
APA
Ren, L., Li, Y., Zhao, Q., Fan, L., Tan, B., Zang, A., & Yang, H. (2020). miR‑519 regulates the proliferation of breast cancer cells via targeting human antigen R. Oncology Letters, 19, 1567-1576. https://doi.org/10.3892/ol.2019.11230
MLA
Ren, L., Li, Y., Zhao, Q., Fan, L., Tan, B., Zang, A., Yang, H."miR‑519 regulates the proliferation of breast cancer cells via targeting human antigen R". Oncology Letters 19.2 (2020): 1567-1576.
Chicago
Ren, L., Li, Y., Zhao, Q., Fan, L., Tan, B., Zang, A., Yang, H."miR‑519 regulates the proliferation of breast cancer cells via targeting human antigen R". Oncology Letters 19, no. 2 (2020): 1567-1576. https://doi.org/10.3892/ol.2019.11230
Copy and paste a formatted citation
x
Spandidos Publications style
Ren L, Li Y, Zhao Q, Fan L, Tan B, Zang A and Yang H: miR‑519 regulates the proliferation of breast cancer cells via targeting human antigen R. Oncol Lett 19: 1567-1576, 2020.
APA
Ren, L., Li, Y., Zhao, Q., Fan, L., Tan, B., Zang, A., & Yang, H. (2020). miR‑519 regulates the proliferation of breast cancer cells via targeting human antigen R. Oncology Letters, 19, 1567-1576. https://doi.org/10.3892/ol.2019.11230
MLA
Ren, L., Li, Y., Zhao, Q., Fan, L., Tan, B., Zang, A., Yang, H."miR‑519 regulates the proliferation of breast cancer cells via targeting human antigen R". Oncology Letters 19.2 (2020): 1567-1576.
Chicago
Ren, L., Li, Y., Zhao, Q., Fan, L., Tan, B., Zang, A., Yang, H."miR‑519 regulates the proliferation of breast cancer cells via targeting human antigen R". Oncology Letters 19, no. 2 (2020): 1567-1576. https://doi.org/10.3892/ol.2019.11230
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