Long-chain non-coding RNA GACAT1 promotes development and progression of breast cancer by targeting microRNA-875-3p
- Qinghua Wang
- Jie Xue
- Qingfang Ren
- Xiaona Li
- Xiaoli Qiu
Affiliations: Department of Breast Surgery, Linyi Cancer Hospital, Linyi, Shandong 276000, P.R. China, Department of Medical Oncology, Linyi Cancer Hospital, Linyi, Shandong 276000, P.R. China
- Published online on: January 24, 2020 https://doi.org/10.3892/ol.2020.11260
Copyright: © Wang
et al. This is an open access article distributed under the
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Role of long‑chain non‑coding ribonucleic acid (lncRNA) GACAT1 in the development of breast cancer and its possible mechanism were investigated. The levels of GACAT1, microRNA‑875‑3p and Stonin2 (STON2) in breast cancer tissues and adjacent normal tissues were detected by quantitative real‑time polymerase chain reaction (qRT‑PCR). The level of GACAT1 in breast cancer cell lines was further explored. The effects of GACAT1 and microRNA‑875‑3p on cell proliferation and cycle were detected by cell counting kit‑8 (CCK‑8) and flow cytometry. The binding relationship between microRNA‑875‑3p and STON2, microRNA‑875‑3p and GACAT1 was examined by a dual luciferase reporting assay. It was confirmed by rescue experiments whether GACAT1 canregulate the level of STON2 by binding to microRNA‑875‑3p. GACAT1 level was clearly enhanced in breast cancer tissues compared to that of the adjacent tissues. Similar result was observed in breast cancer cell lines. Upregulation of GACAT1 promoted the proliferation and cycle of breast cancer cells including MCF‑7 and BCap‑37. The dual luciferase reporting assay results indicated that GACAT1 had a binding relationship with microRNA‑875‑3p. Further experiments confirmed that microRNA‑875‑3p was conspicuously downregulated in breast cancer tissues, and upregulation of microRNA‑875‑3p could inhibit the proliferation ability of MCF‑7 and BCap‑37 cells, and partially reversed the promoting effect of GACAT1 on cell cycle. Through bioinformatics prediction and dual luciferase reporter gene experiments, we found that STON2 might be a target gene of microRNA‑875‑3p. Overexpression of STON2 could partially abolish the effect of microRNA‑875‑3p on cell proliferation and cycle of MCF‑7 and BCap‑37 cells. GACAT1 can participate in the progression of breast cancer by promoting the proliferation and cycle of breast cancer cells. The mechanism may be through the regulation of the level of STON2 by adsorbing microRNA‑875‑3p.