Effect of miR‑205 on proliferation and migration of thyroid cancer cells by targeting CCNB2 and the mechanism

Wang,Xin; Zhang,Haomin; Jiao,Kai; Zhao,Chunyang; Liu,Hailong; Meng,Qinghong; Wang,Zhao; Feng,Chunling; Li,Yuanchun

doi:10.3892/ol.2020.11275

Effect of miR‑205 on proliferation and migration of thyroid cancer cells by targeting CCNB2 and the mechanism

Authors:
- Xin Wang
- Haomin Zhang
- Kai Jiao
- Chunyang Zhao
- Hailong Liu
- Qinghong Meng
- Zhao Wang
- Chunling Feng
- Yuanchun Li
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Affiliations: Department of General Surgery, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161006, P.R. China, Department of Oncological Surgery, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161006, P.R. China, Department of Obstetrics and Gynecology, Qiqihar Second Hospital, Qiqihar, Heilongjiang 161006, P.R. China, Department of Cardiology, Qiqihar First Hospital, Qiqihar, Heilongjiang 161005, P.R. China
Published online on: January 24, 2020 https://doi.org/10.3892/ol.2020.11275
Pages: 2568-2574
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

This study explored the target of miR‑205 and the effect of miR‑205 on the proliferation and migration regulating its target in thyroid cancer cells (TC). Twenty‑five pairs of TC and adjacent tissues were collected after surgical resection. Real‑time fluorescence quantitative PCR (qRT‑PCR) was used to detect the expression of miR‑205 in TC tissues and cells (SW579, B‑CPAP, TPC‑1, WRO). SW579 cells were transfected with miR‑205 mimic, and SW579 cells with overexpression of miR‑205 were constructed. The effects of miR‑205 overexpression on the proliferation and migration of SW579 cells were observed by cell counting kit‑8 (CCK‑8) and Transwell assays, respectively. Luciferase reporter assay was further used to look for the target of miR‑205 and to study the mechanism of miR‑205 in the proliferation and migration of TC cells. Compared with normal tissues and cells, the expression of miR‑205 was significantly reduced in TC tissues (t=3.47, P=0.031) and cells (t=5.41, P=0.016). Overexpression of miR‑205 inhibited the proliferation (t=4.12, P=0.035) and migration (t=4.47, P=0.027) of SW579 cells. Luciferase reporter assays found that CCNB2 was a target gene of miR‑205 (t=4.63, P=0.024), qRT‑PCR and western blot assays confirmed there was negatively correlation between CCNB2 and miR‑205 (t=3.55, P=0.029; t=2.86, P=0.043). CCNB2 overexpression reversed the inhibition of miR‑205 on the proliferation (t=3.70, P=0.031) and migration (t=4.12, P=0.022) of SW579 cells. In conclusion, miR‑205 inhibits the proliferation and migration of TC cells by targeting CCNB2, which may be a potential target of TC therapy.

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