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Effect of miR‑205 on proliferation and migration of thyroid cancer cells by targeting CCNB2 and the mechanism

  • Authors:
    • Xin Wang
    • Haomin Zhang
    • Kai Jiao
    • Chunyang Zhao
    • Hailong Liu
    • Qinghong Meng
    • Zhao Wang
    • Chunling Feng
    • Yuanchun Li
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161006, P.R. China, Department of Oncological Surgery, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161006, P.R. China, Department of Obstetrics and Gynecology, Qiqihar Second Hospital, Qiqihar, Heilongjiang 161006, P.R. China, Department of Cardiology, Qiqihar First Hospital, Qiqihar, Heilongjiang 161005, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2568-2574
    |
    Published online on: January 24, 2020
       https://doi.org/10.3892/ol.2020.11275
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Abstract

This study explored the target of miR‑205 and the effect of miR‑205 on the proliferation and migration regulating its target in thyroid cancer cells (TC). Twenty‑five pairs of TC and adjacent tissues were collected after surgical resection. Real‑time fluorescence quantitative PCR (qRT‑PCR) was used to detect the expression of miR‑205 in TC tissues and cells (SW579, B‑CPAP, TPC‑1, WRO). SW579 cells were transfected with miR‑205 mimic, and SW579 cells with overexpression of miR‑205 were constructed. The effects of miR‑205 overexpression on the proliferation and migration of SW579 cells were observed by cell counting kit‑8 (CCK‑8) and Transwell assays, respectively. Luciferase reporter assay was further used to look for the target of miR‑205 and to study the mechanism of miR‑205 in the proliferation and migration of TC cells. Compared with normal tissues and cells, the expression of miR‑205 was significantly reduced in TC tissues (t=3.47, P=0.031) and cells (t=5.41, P=0.016). Overexpression of miR‑205 inhibited the proliferation (t=4.12, P=0.035) and migration (t=4.47, P=0.027) of SW579 cells. Luciferase reporter assays found that CCNB2 was a target gene of miR‑205 (t=4.63, P=0.024), qRT‑PCR and western blot assays confirmed there was negatively correlation between CCNB2 and miR‑205 (t=3.55, P=0.029; t=2.86, P=0.043). CCNB2 overexpression reversed the inhibition of miR‑205 on the proliferation (t=3.70, P=0.031) and migration (t=4.12, P=0.022) of SW579 cells. In conclusion, miR‑205 inhibits the proliferation and migration of TC cells by targeting CCNB2, which may be a potential target of TC therapy.
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1 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Cabanillas ME, McFadden DG and Durante C: Thyroid cancer. Lancet. 388:2783–2795. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Kong YW, Ferland-McCollough D, Jackson TJ and Bushell M: microRNAs in cancer management. Lancet Oncol. 13:e249–e258. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Rupaimoole R and Slack FJ: MicroRNA therapeutics: Towards a new era for the management of cancer and other diseases. Nat Rev Drug Discov. 16:203–222. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Wang L, Kang FB, Wang J, Yang C and He DW: Downregulation of miR-205 contributes to epithelial-mesenchymal transition and invasion in triple-negative breast cancer by targeting HMGB1-RAGE signaling pathway. Anticancer Drugs. 30:225–232. 2019. View Article : Google Scholar : PubMed/NCBI

6 

Wu F, Xing T, Gao X and Liu F: miR-501-3p promotes colorectal cancer progression via activation of Wnt/β-catenin signaling. Int J Oncol. 55:671–683. 2019.PubMed/NCBI

7 

Yao L, Shi W and Gu J: Micro-RNA 205-5p is involved in the progression of gastric cancer and targets phosphatase and tensin homolog (PTEN) in SGC-7901 human gastric cancer cells. Med Sci Monit. 25:6367–6377. 2019. View Article : Google Scholar : PubMed/NCBI

8 

Hu A, Zhang Y, Zhao X, Li J and Ying Y: CBX1 is a direct target of miR-205-5p and contributes to the progression of pituitary tumor. Pharmazie. 74:154–156. 2019.PubMed/NCBI

9 

Chen S, Jin L, Nie S, Han L, Lu N and Zhou Y: miR-205 inhibits neuroblastoma growth by targeting cAMP-responsive element-binding protein 1. Oncol Res. 26:445–455. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Yang X, Yang L, Ma Y, Zhao X and Wang H: MicroRNA-205 mediates proteinase-activated receptor 2 (PAR2)-promoted cancer cell migration. Cancer Invest. 35:601–609. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Pang H and Yue X: MiR-205 serves as a prognostic factor and suppresses proliferation and invasion by targeting insulin-like growth factor receptor 1 in human cervical cancer. Tumour Biol. Jun 27–2017.(Epub ahead of print). doi org/10.1177/1010428317701308. View Article : Google Scholar

12 

Liu Y, Qian XM, He QC and Weng JK: MiR-421 inhibition protects H9c2 cells against hypoxia/reoxygenation-induced oxidative stress and apoptosis by targeting Sirt3. Perfusion. Aug 30–2019.(Epub ahead of print). doi: 10.1177/0267659119870725. View Article : Google Scholar

13 

Mei JW, Yang ZY, Xiang HG, Bao R, Ye YY, Ren T, Wang XF and Shu YJ: MicroRNA-1275 inhibits cell migration and invasion in gastric cancer by regulating vimentin and E-cadherin via JAZF1. BMC Cancer. 19:7402019. View Article : Google Scholar : PubMed/NCBI

14 

Ye K, Xu C and Hui T: MiR-34b inhibits the proliferation and promotes apoptosis in colon cancer cells by targeting Wnt/β-catenin signaling pathway. Biosci Rep. 39:BSR201917992019. View Article : Google Scholar : PubMed/NCBI

15 

Jiao D, Guo F and Fu Q: MicroRNA 873 inhibits the progression of thyroid cancer by directly targeting ZEB1. Mol Med Rep. 20:1986–1993. 2019.PubMed/NCBI

16 

Guo H and Zhang L: MicroRNA-30a suppresses papillary thyroid cancer cell proliferation, migration and invasion by directly targeting E2F7. Exp Ther Med. 18:209–215. 2019.PubMed/NCBI

17 

Dai B, Zhou G, Hu Z, Zhu G, Mao B, Su H and Jia Q: MiR-205 suppresses epithelial-mesenchymal transition and inhibits tumor growth of human glioma through downregulation of HOXD9. Biosci Rep. 39:392019. View Article : Google Scholar

18 

Zhuang L, Guo J, Yao Y and Li Z: miR-205 targets runt-related transcription factor 2 to inhibit human pancreatic cancer progression. Oncol Lett. 17:843–848. 2019.PubMed/NCBI

19 

Lu J, Lin Y, Li F, Ye H, Zhou R, Jin Y, Li B, Xiong X and Cheng N: MiR-205 suppresses tumor growth, invasion, and epithelial-mesenchymal transition by targeting SEMA4C in hepatocellular carcinoma. FASEB J. May 25–2018.(Epub ahead of print). doi: 10.1096/fj.201800113R. View Article : Google Scholar

20 

Ballew O and Lacefield S: The DNA damage checkpoint and the spindle position checkpoint: Guardians of meiotic commitment. Curr Genet. 65:1135–1140. 2019. View Article : Google Scholar : PubMed/NCBI

21 

Hochegger H, Takeda S and Hunt T: Cyclin-dependent kinases and cell-cycle transitions: Does one fit all? Nat Rev Mol Cell Biol. 9:910–916. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Qian X, Song X, He Y, Yang Z, Sun T, Wang J, Zhu G, Xing W and You C: CCNB2 overexpression is a poor prognostic biomarker in Chinese NSCLC patients. Biomed Pharmacother. 74:222–227. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Lei CY, Wang W, Zhu YT, Fang WY and Tan WL: The decrease of cyclin B2 expression inhibits invasion and metastasis of bladder cancer. Urol Oncol. 34:237.e1–237.e10. 2016. View Article : Google Scholar

24 

Shi Q, Wang W, Jia Z, Chen P, Ma K and Zhou C: ISL1, a novel regulator of CCNB1, CCNB2 and c-MYC genes, promotes gastric cancer cell proliferation and tumor growth. Oncotarget. 7:36489–36500. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Shubbar E, Kovács A, Hajizadeh S, Parris TZ, Nemes S, Gunnarsdóttir K, Einbeigi Z, Karlsson P and Helou K: Elevated cyclin B2 expression in invasive breast carcinoma is associated with unfavorable clinical outcome. BMC Cancer. 13:12013. View Article : Google Scholar : PubMed/NCBI

26 

Li R, Jiang X, Zhang Y, Wang S, Chen X, Yu X, Ma J and Huang X: Cyclin B2 overexpression in human hepatocellular carcinoma is associated with poor prognosis. Arch Med Res. 50:10–17. 2019. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Zhang H, Jiao K, Zhao C, Liu H, Meng Q, Wang Z, Feng C and Li Y: Effect of miR‑205 on proliferation and migration of thyroid cancer cells by targeting CCNB2 and the mechanism. Oncol Lett 19: 2568-2574, 2020.
APA
Wang, X., Zhang, H., Jiao, K., Zhao, C., Liu, H., Meng, Q. ... Li, Y. (2020). Effect of miR‑205 on proliferation and migration of thyroid cancer cells by targeting CCNB2 and the mechanism. Oncology Letters, 19, 2568-2574. https://doi.org/10.3892/ol.2020.11275
MLA
Wang, X., Zhang, H., Jiao, K., Zhao, C., Liu, H., Meng, Q., Wang, Z., Feng, C., Li, Y."Effect of miR‑205 on proliferation and migration of thyroid cancer cells by targeting CCNB2 and the mechanism". Oncology Letters 19.3 (2020): 2568-2574.
Chicago
Wang, X., Zhang, H., Jiao, K., Zhao, C., Liu, H., Meng, Q., Wang, Z., Feng, C., Li, Y."Effect of miR‑205 on proliferation and migration of thyroid cancer cells by targeting CCNB2 and the mechanism". Oncology Letters 19, no. 3 (2020): 2568-2574. https://doi.org/10.3892/ol.2020.11275
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Zhang H, Jiao K, Zhao C, Liu H, Meng Q, Wang Z, Feng C and Li Y: Effect of miR‑205 on proliferation and migration of thyroid cancer cells by targeting CCNB2 and the mechanism. Oncol Lett 19: 2568-2574, 2020.
APA
Wang, X., Zhang, H., Jiao, K., Zhao, C., Liu, H., Meng, Q. ... Li, Y. (2020). Effect of miR‑205 on proliferation and migration of thyroid cancer cells by targeting CCNB2 and the mechanism. Oncology Letters, 19, 2568-2574. https://doi.org/10.3892/ol.2020.11275
MLA
Wang, X., Zhang, H., Jiao, K., Zhao, C., Liu, H., Meng, Q., Wang, Z., Feng, C., Li, Y."Effect of miR‑205 on proliferation and migration of thyroid cancer cells by targeting CCNB2 and the mechanism". Oncology Letters 19.3 (2020): 2568-2574.
Chicago
Wang, X., Zhang, H., Jiao, K., Zhao, C., Liu, H., Meng, Q., Wang, Z., Feng, C., Li, Y."Effect of miR‑205 on proliferation and migration of thyroid cancer cells by targeting CCNB2 and the mechanism". Oncology Letters 19, no. 3 (2020): 2568-2574. https://doi.org/10.3892/ol.2020.11275
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