Long non‑coding RNA EGFR‑AS1 sponges micorRNA‑381 to upregulate ROCK2 in bladder cancer
- Shouxian Yuan
- Xiuhua Luan
- Haixia Chen
- Xiuqing Shi
- Xiangkai Zhang
Affiliations: Department of Urology Surgery, The Second People's Hospital of Liaocheng, Linqing, Shandong 252600, P.R. China, Department of Medical Records, The Second People's Hospital of Liaocheng, Linqing, Shandong 252600, P.R. China, Department of Urology Surgery, Shanxi Provincial People's Hospital, Taiyuan, Shanxi 030012, P.R. China
- Published online on: January 9, 2020 https://doi.org/10.3892/ol.2020.11283
Copyright: © Yuan
et al. This is an open access article distributed under the
terms of Creative
Commons Attribution License.
Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
The present study aimed to investigate the role of the long non‑coding RNA EGFR‑AS1 in bladder cancer (BC). In this study gene expression of both BC and non‑tumor tissues from BC patients were measured by quantitative PCR. Cell transfections were performed to analyze gene interactions in HT‑1197 cells. Transwell assays were performed to analyze cell invasion and migration of HT‑1197 cells. It was revealed that epidermal growth factor receptor‑antisense RNA 1 (EGFR‑AS1) was upregulated in BC and positively associated with rho associated coiled‑coil containing protein kinase 2 (ROCK2). Analysis of data collected in follow‑ups indicated that EGFR‑AS1 expression was significantly associated with poorer overall survival of patients with BC. Moreover, in bladder cancer cells, EGFR‑AS1 overexpression mediated the upregulation of ROCK2, while microRNA (miR)‑381 mediated the downregulation of ROCK2. However, EGFR‑AS1 and ROCK2 failed to affect each other. Bioinformatics analysis indicated that miR‑381 binds EGFR‑AS1. In addition, EGFR‑AS1 and ROCK2 overexpression resulted in the promotion of cell invasiveness and migration of HT‑1197 BC cells. Conversely, miR‑381 was revealed to partially reverse the effect of EGFR‑AS1 overexpression. Therefore, EGFR‑AS1 may sponge miR‑381 to upregulate ROCK2 in BC, thereby promoting cell invasion and migration.