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Article

Akt inhibitor MK‑2206 reduces pancreatic cancer cell viability and increases the efficacy of gemcitabine

  • Authors:
    • Zhanshan Wang
    • Guangtao Luo
    • Zhengjun Qiu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Shanghai General Hospital of Nanjing Medical University, Shanghai 200080, P.R. China, Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China
  • Pages: 1999-2004
    |
    Published online on: January 14, 2020
       https://doi.org/10.3892/ol.2020.11300
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Abstract

The PI3K/Akt pathway is an attractive therapeutic target in the treatment of pancreatic cancer, as it was demonstrated to be aberrantly regulated in pancreatic cancer cells. The present study aimed to investigate the therapeutic potential of the novel Akt inhibitor MK‑2206 in human pancreatic cancer cell lines. Pancreatic cancer cell survival following MK‑2206 treatment was assessed using the Cell Counting Kit‑8 (CCK‑8) assay, colony formation and determination of the apoptotic rate by flow cytometry following annexin‑V‑fluorescein isothiocyanate/propidium iodide staining. The effects of MK‑2206 alone or in combination with gemcitabine on pancreatic cell proliferation were assessed using the CCK‑8 assay. Western blotting was used to examine the effects of the two drugs on Akt protein expression. The results demonstrated that MK‑2206 inhibited the proliferation and induced apoptosis of the Mia PaCa‑2 and Panc‑1 pancreatic cancer cell lines. In addition, CCK‑8 cytotoxicity test showed that combined administration of MK‑2206 with gemcitabine enhanced the cytotoxic efficacy of gemcitabine. Furthermore, a low dose of MK‑2206 (1 µM) combined with gemcitabine was enough to inhibit Akt phosphorylation. Taken together, these results provided some insight into the underlying mechanism of the anticancer effects of MK‑2206 on pancreatic cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Z, Luo G and Qiu Z: Akt inhibitor MK‑2206 reduces pancreatic cancer cell viability and increases the efficacy of gemcitabine. Oncol Lett 19: 1999-2004, 2020.
APA
Wang, Z., Luo, G., & Qiu, Z. (2020). Akt inhibitor MK‑2206 reduces pancreatic cancer cell viability and increases the efficacy of gemcitabine. Oncology Letters, 19, 1999-2004. https://doi.org/10.3892/ol.2020.11300
MLA
Wang, Z., Luo, G., Qiu, Z."Akt inhibitor MK‑2206 reduces pancreatic cancer cell viability and increases the efficacy of gemcitabine". Oncology Letters 19.3 (2020): 1999-2004.
Chicago
Wang, Z., Luo, G., Qiu, Z."Akt inhibitor MK‑2206 reduces pancreatic cancer cell viability and increases the efficacy of gemcitabine". Oncology Letters 19, no. 3 (2020): 1999-2004. https://doi.org/10.3892/ol.2020.11300
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Luo G and Qiu Z: Akt inhibitor MK‑2206 reduces pancreatic cancer cell viability and increases the efficacy of gemcitabine. Oncol Lett 19: 1999-2004, 2020.
APA
Wang, Z., Luo, G., & Qiu, Z. (2020). Akt inhibitor MK‑2206 reduces pancreatic cancer cell viability and increases the efficacy of gemcitabine. Oncology Letters, 19, 1999-2004. https://doi.org/10.3892/ol.2020.11300
MLA
Wang, Z., Luo, G., Qiu, Z."Akt inhibitor MK‑2206 reduces pancreatic cancer cell viability and increases the efficacy of gemcitabine". Oncology Letters 19.3 (2020): 1999-2004.
Chicago
Wang, Z., Luo, G., Qiu, Z."Akt inhibitor MK‑2206 reduces pancreatic cancer cell viability and increases the efficacy of gemcitabine". Oncology Letters 19, no. 3 (2020): 1999-2004. https://doi.org/10.3892/ol.2020.11300
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