Long non‑coding RNA ELF3‑antisense RNA 1 promotes osteosarcoma cell proliferation by upregulating Kruppel‑like factor 12 potentially via methylation of the microRNA‑205 gene

Yuan,Jianmin; Kang,Jianhui; Yang,Min

doi:10.3892/ol.2020.11312

Long non‑coding RNA ELF3‑antisense RNA 1 promotes osteosarcoma cell proliferation by upregulating Kruppel‑like factor 12 potentially via methylation of the microRNA‑205 gene

Authors:
- Jianmin Yuan
- Jianhui Kang
- Min Yang
View Affiliations

Affiliations: Department of Orthopedics and Traumatology, First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241001, P.R. China, Department of Cardiology, First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241001, P.R. China
Published online on: January 16, 2020 https://doi.org/10.3892/ol.2020.11312
Pages: 2475-2480

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

A recent study characterized the long non‑coding RNA (lncRNA) ELF3‑antisense RNA 1 (ELF3‑AS1) as an oncogenic lncRNA in bladder cancer. The present study aimed to investigate the role of ELF3‑AS1 in osteosarcoma (OS). It was found that ELF3‑AS1 was upregulated in OS tissues, and ELF3‑AS1 expression level increased with increasing clinical stage. In OS tissues, Kruppel‑like factor 12 (KLF12) was positively correlated with ELF3‑AS1, while microRNA (miR)‑205 was negatively correlated with ELF3‑AS1. ELF3‑AS1 overexpression resulted in the upregulation of KLF12, but the downregulation of miR‑205. Overexpression of miR‑205 caused downregulation of KLF12, but had no significant effects on ELF3‑AS1 expression. Overexpression of KLF12 showed no significant impact on ELF3‑AS1 and miR‑205. ELF3‑AS1 and KLF12 overexpression resulted in an increased proliferation rate in OS cells, while miR‑205 played an opposite role and attenuated the effects of ELF3‑AS1 overexpression. ELF3‑AS1 overexpression promoted the methylation of the miR‑205 gene. Therefore, ELF3‑AS1 may promote OS cell proliferation by upregulating KLF12 through the methylation of the miR‑205 gene.

View Figures

View References

1	Geller DS and Gorlick R: Osteosarcoma: A review of diagnosis, management, and treatment strategies. Clin Adv Hematol Oncol. 8:705–718. 2010.PubMed/NCBI
2	Messerschmitt PJ, Garcia RM, Abdul-Karim FW, Greenfield EM and Getty PJ: Osteosarcoma. J Am Acad Orthop Surg. 17:515–527. 2009. View Article : Google Scholar : PubMed/NCBI
3	Mirabello L, Troisi RJ and Savage SA: International osteosarcoma incidence patterns in children and adolescents, middle ages and elderly persons. Int J Cancer. 125:229–234. 2009. View Article : Google Scholar : PubMed/NCBI
4	Jaffe N: Osteosarcoma: Review of the past, impact on the future. The American experience. Cancer Treat Res. 152:239–262. 2009. View Article : Google Scholar : PubMed/NCBI
5	Daw NC, Billups CA, Rodriguez-Galindo C, McCarville MB, Rao BN, Cain AM, Jenkins JJ, Neel MD and Meyer WH: Metastatic osteosarcoma. Cancer. 106:403–412. 2006. View Article : Google Scholar : PubMed/NCBI
6	Kansara M and Thomas DM: Molecular pathogenesis of osteosarcoma. DNA Cell Biol. 26:1–18. 2007. View Article : Google Scholar : PubMed/NCBI
7	Hansen MF: Genetic and molecular aspects of osteosarcoma. J Musculoskelet Neuronal Interact. 2:554–560. 2002.PubMed/NCBI
8	Reddy KB: MicroRNA (miRNA) in cancer. Cancer Cell Int. 15:382015. View Article : Google Scholar : PubMed/NCBI
9	McManus MT: MicroRNAs and cancer. Semin Cancer Biol. 13:253–258. 2003. View Article : Google Scholar : PubMed/NCBI
10	Yang G, Lu X and Yuan L: LncRNA: A link between RNA and cancer. Biochim Biophys Acta. 1839:1097–1109. 2014. View Article : Google Scholar : PubMed/NCBI
11	Esteller M: Non-coding RNAs in human disease. Nat Rev Genet. 12:861–874. 2011. View Article : Google Scholar : PubMed/NCBI
12	Guo Y, Chen D, Su X, Chen J and Li Y: The lncRNA ELF3-AS1 promotes bladder cancer progression by interaction with Krüppel-like factor 8. Biochem Biophys Res Commun. 508:762–768. 2019. View Article : Google Scholar : PubMed/NCBI
13	Hezova R, Kovarikova A, Srovnal J, Zemanova M, Harustiak T, Ehrmann J, Hajduch M, Sachlova M, Svoboda M and Slaby O: MiR-205 functions as a tumor suppressor in adenocarcinoma and an oncogene in squamous cell carcinoma of esophagus. Tumuor Biol. 37:8007–8018. 2016. View Article : Google Scholar
14	Li J, Hu K, Gong G, Zhu D, Wang Y, Liu H and Wu X: Upregulation of MiR-205 transcriptionally suppresses SMAD4 and PTEN and contributes to human ovarian cancer progression. Sci Rep. 7:413302017. View Article : Google Scholar : PubMed/NCBI
15	Guan B, Li Q, Shen L, Rao Q, Wang Y, Zhu Y, Zhou XJ and Li XH: MicroRNA-205 directly targets Krüppel-like factor 12 and is involved in invasion and apoptosis in basal-like breast carcinoma. Int J Oncol. 49:720–734. 2016. View Article : Google Scholar : PubMed/NCBI
16	Steffner RJ and Jang ES: Staging of bone and soft-tissue sarcomas. J Am Acad Orthop Surg. 26:e269–e278. 2018. View Article : Google Scholar : PubMed/NCBI
17	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
18	Kim SH, Park YY, Cho SN, Margalit O, Wang D and DuBois RN: Krüppel-like factor 12 promotes colorectal cancer growth through early growth response protein 1. PLoS One. 11:e01598992016. View Article : Google Scholar : PubMed/NCBI
19	Liang WC, Fu WM, Wong CW, Wang Y, Wang WM, Hu GX, Zhang L, Xiao LJ, Wan DC, Zhang JF and Waye MM: The lncRNA H19 promotes epithelial to mesenchymal transition by functioning as miRNA sponges in colorectal cancer. Oncotarget. 6:22513–22525. 2015. View Article : Google Scholar : PubMed/NCBI
20	Liz J and Esteller M: lncRNAs and microRNAs with a role in cancer development. Biochim Biophys Acta. 1859:169–176. 2016. View Article : Google Scholar : PubMed/NCBI