Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
April-2020 Volume 19 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2020 Volume 19 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Decreased IL‑37 expression in hepatocellular carcinoma tissues and liver cancer cell lines

  • Authors:
    • Peng Li
    • Hongsheng Guo
    • Kun Wu
    • Liudan Su
    • Kai Huang
    • Ruizhi Lai
    • Ziliang Deng
    • Shuxian Li
    • Ping Ouyang
    • Yan Wang
    • Zhangquan Chen
    • Guangji Zhou
    • Sen Wang
  • View Affiliations / Copyright

    Affiliations: Department of Histology and Embryology, Basic Medical College, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China, Department of Internal Medicine, The Second Clinical Medical College, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China, Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2639-2648
    |
    Published online on: February 13, 2020
       https://doi.org/10.3892/ol.2020.11393
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The role of IL‑37 in cancer is currently largely unknown. The present study aimed to investigate IL‑37 expression in hepatocellular carcinoma (HCC), paracancerous tissues (PT) and liver cancer cell lines, and their associations between IL‑37 and NF‑κB. A total of 65 HCC and 65 PT tissues were collected. The expression of IL‑37 and NF‑κB in tissues was detected by immunohistochemistry (IHC) and the data was analyzed using SPSS software. In the in vitro studies, IL‑37 gene was transfected into HepG2 and MHCC97H cell lines with Lipofectamine 3000, and the protein regulation of NF‑κB by IL‑37 was verified by immunofluorescence (IF) and western blotting. In HCC, the positive expression rates of IL‑37 and NF‑kB were 21.5 and 95.4%, respectively. In PT, strong positive staining of IL‑37and weak positive staining of NF‑κB were observed. The normal expression levels of IL‑37 and NF‑κB, the increased IL‑37 and decreased NF‑κB induced by IL‑37 gene transfection were observed through IF in cell lines. In terms of clinical significance, the difference in IL‑37 expression between HCC and PT was statistically significant (χ2=55.05; P<0.001). IL‑37 expression in HCC but not PT was negatively associated with serum AFP (χ2=6.522; P=0.039). IL‑37 expression in PT was associated with sex (χ2=13.12; P=0.003) and tumor size (χ2=7.996; P=0.045). NF‑κB expression in PT was associated with age, sex and BCLC stage. Notably, there was a negative correlation between IL‑37 and NF‑κB in HCC (r=‑0.277; P=0.029) but not in PT (P>0.05). IL‑37 overexpression downregulated the NF‑κB protein by 56.50% in HepG2 cells (P<0.05) and 30.52% in MHCC97H cells (P<0.05). In conclusion, the expression of IL‑37 in HCC and PT was specifically associated with serum AFP and tumor size, respectively. IL‑37 expression was negatively correlated with NF‑κB protein expression in HCC tissues and liver cancer cell lines.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Wong MC, Jiang JY, Goggins WB, Liang M, Fang Y, Fung FD, Leung C, Wang HH, Wong GL, Wong VW and Chan HL: International incidence and mortality trends of liver cancer: A global profile. Sci Rep. 7:458462017. View Article : Google Scholar : PubMed/NCBI

2 

Cheuk-Fung Yip T, Wai-Sun Wong V, Lik-Yuen Chan H, Tse YK, Pik-Shan Kong A, Long-Yan Lam K, Chung-Yan Lui G and Lai-Hung Wong G: Effects of diabetes and glycemic control on risk of hepatocellular carcinoma after seroclearance of hepatitis B surface antigen. Clin Gastroenterol Hepatol. 16:765–773. 2018. View Article : Google Scholar : PubMed/NCBI

3 

Saha Turna N and Wu F: Risk assessment of aflatoxin-related liver cancer in Bangladesh. Food Additi Contam Part A Chem Anal Control Expo Risk Assess. 36:320–326. 2019. View Article : Google Scholar

4 

Kawamura Y, Arase Y, Ikeda K, Akuta N, Kobayashi M, Saitoh S, Suzuki F, Suzuki Y, Inao M, Mochida S and Kumada H: Effects of alcohol consumption on hepatocarcinogenesis in Japanese patients with fatty liver disease. Clin Gastroenterol Hepatol. 14:597–605. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Taniguchi K and Karin M: NF-κB, inflammation, immunity and cancer: Coming of age. Nat Rev Immunol. 18:309–324. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Banchereau J, Pascual V and O'garra A: From IL-2 to IL-37: The expanding spectrum of anti-inflammatory cytokines. Nat Immunol. 13:925–931. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Wang WQ, Dong K, Zhou L, Jiao GH, Zhu CZ, Li WW, Yu G, Wu WT, Chen S, Sun ZN, et al: IL-37b gene transfer enhances the therapeutic efficacy of mesenchumal stromal cells in DSS-induced colitis mice. Acta Pharm Sin. 36:1377–1387. 2015. View Article : Google Scholar

8 

Wang L, Wang Y, Xia L, Shen H and Lu J: Elevated frequency of IL-37-and IL-18Rα-positive T cells in the peripheral blood of rheumatoid arthritis patients. Cytokine. 110:291–297. 2018. View Article : Google Scholar : PubMed/NCBI

9 

Wang J, Shen Y, Li C, Liu C, Wang ZH, Li YS, Ke X and Hu GH: IL-37 attenuates allergic process via STAT6/STAT3 pathways in murine allergic rhinitis. Int Immunopharmacol. 69:27–33. 2019. View Article : Google Scholar : PubMed/NCBI

10 

de Sousa JR, Prudente RL, Dias Junior LB, Oliveira Carneiro FR, Sotto MN and Simoes Quaresma JA: IL-37 and leprosy: A novel cytokine involved in the host response to mycobacterium leprae infection. Cytokine. 106:89–94. 2018. View Article : Google Scholar : PubMed/NCBI

11 

Ye L, Ji L, Wen Z, Zhou Y, Hu D, Li Y, Yu T, Chen B, Zhang J, Ding L, Du J, Huang Z, et al: IL-37 inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells of patients with systemic lupus erythematosus: Its correlation with disease activity. J Transl Med. 12:692014. View Article : Google Scholar : PubMed/NCBI

12 

Yang Y, Zhang ZX, Lian D, Haig A, Bhattacharjee RN and Jevnikar AM: IL-37 inhibits IL-18-induced tubular epithelial cell expression of pro-inflammatory cytokines and renal ischemia-reperfusion injury. Kidney Int. 87:396–408. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Bulau AM, Fink M, Maucksch C, Kappler R, Mayr D, Wagner K and Bufler P: In vivo expression of interleukin-37 reduces local and systemic inflammation in concanavalin A-induced hepatitis. ScientificWorldJournal. 11:2480–2490. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Feng XX, Chi G, Wang H, Gao Y, Chen Q, Ru YX, Luo ZL, Yan W, Li PY, Liu M, et al: IL-37 suppresses the sustained hepatic IFN-γ/TNF-α production and T cell-dependent liver injury. Int Immunopharmacol. 69:184–193. 2019. View Article : Google Scholar : PubMed/NCBI

15 

Wang S, An W, Yao Y, Chen R, Zheng X, Yang W, Zhao Y, Hu X, Jiang E, Bie Y, et al: Interleukin 37 expression inhibits STAT3 to suppress the proliferation and invasion of human cervical cancer cells. J Cancer. 6:962–969. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Ouyang P, An W, Chen R, Zhang H, Chen D, Jiang E, Zhu W, Li P, Guo H, Chen Z and Wang S: IL-37 promotes cell apoptosis in cervical cancer involving bim upregulation. OncoTargets Ther. 12:2703–2712. 2019. View Article : Google Scholar

17 

Jiang M, Wang Y, Zhang H, Ji Y, Zhao P, Sun R and Zhang C: IL-37 inhibits invasion and metastasis in nonsmall cell lung cancer by suppressing the IL-6/STAT3 signaling pathway. Thoracic Cancer. 9:621–629. 2018. View Article : Google Scholar : PubMed/NCBI

18 

Wu T, Xu B, Zhao G, Luo J and Luo C: IL-37 suppresses migration and invasion of gallbladder cancer cells through inhibition of HIF-1α induced epithelial-mesenchymal transition. Eur Rev Med Pharm Sci. 22:8179–8185. 2018.

19 

Zhu B, Zhang P, Liu M, Jiang C, Liu H and Fu J: Prognostic significance of CSN2, CD8, and MMR status-associated nomograms in patients with colorectal cancer. Transl Oncol. 11:1202–1212. 2018. View Article : Google Scholar : PubMed/NCBI

20 

Zhao JJ, Pan QZ, Pan K, Weng DS, Wang QJ, Li JJ, Lv L, Wang DD, Zheng HX, Jiang SS, et al: Interleukin-37 mediates the antitumor activity in hepatocellular carcinoma: Role for CD57+ NK cells. Sci Rep. 4:51772014. View Article : Google Scholar : PubMed/NCBI

21 

Li P, Guo H, Zhou G, Shi H, Li Z, Guan X, Deng Z, Li S, Zhou S, Wang Y and Wang S: Increased ZNF84 expression in cervical cancer. Arch Gynecol Obstet. 297:1525–1532. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Chan AW, Kumada T, Toyoda H, Tada T, Chong CC, Mo FK, Yeo W, Johnson PJ, Lai PB, Chan AT, et al: Integration of albumin-bilirubin (ALBI) score into barcelona clinic liver cancer (BCLC) system for hepatocellular carcinoma. J Gastroenterol Hepatol. 31:1300–1306. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Yoneda M, Hyun J, Jakubski S, Saito S, Nakajima A, Schiff ER and Thomas E: Hepatitis B virus and DNA stimulation trigger a rapid innate immune response through NF-κB. J Immunol. 197:630–643. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Yan X, Zhao J and Zhang R: Interleukin-37 mediates the antitumor activity in colon cancer through β-catenin suppression. Oncotarget. 8:490642017.PubMed/NCBI

25 

Ge G, Wang A, Yang J, Chen Y, Yang J, Li Y and Xue Y: Interleukin-37 suppresses tumor growth through inhibition of angiogenesis in non-small cell lung cancer. J Exp Clin Cancer Res. 35:132016. View Article : Google Scholar : PubMed/NCBI

26 

Ma H, Sun X, Chen L, Cheng W, Han XX, Zhao B and He C: Multiplex immunochips for high-accuracy detection of AFP-L3% based on surface-enhanced raman scattering: Implications for early liver cancer diagnosis. Anal Chem. 89:8877–8883. 2017. View Article : Google Scholar : PubMed/NCBI

27 

Yang JY, Li X, Gao L, Teng ZH and Liu WC: Co-transfection of dendritic cells with AFP and IL-2 genes enhances the induction of tumor antigen-specific antitumor immunity. Exp Ther Med. 4:655–660. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Xia Y, Shen S and Verma IM: NF-κB, an active player in human cancers. Cancer Immunol Res. 2:823–830. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li P, Guo H, Wu K, Su L, Huang K, Lai R, Deng Z, Li S, Ouyang P, Wang Y, Wang Y, et al: Decreased IL‑37 expression in hepatocellular carcinoma tissues and liver cancer cell lines. Oncol Lett 19: 2639-2648, 2020.
APA
Li, P., Guo, H., Wu, K., Su, L., Huang, K., Lai, R. ... Wang, S. (2020). Decreased IL‑37 expression in hepatocellular carcinoma tissues and liver cancer cell lines. Oncology Letters, 19, 2639-2648. https://doi.org/10.3892/ol.2020.11393
MLA
Li, P., Guo, H., Wu, K., Su, L., Huang, K., Lai, R., Deng, Z., Li, S., Ouyang, P., Wang, Y., Chen, Z., Zhou, G., Wang, S."Decreased IL‑37 expression in hepatocellular carcinoma tissues and liver cancer cell lines". Oncology Letters 19.4 (2020): 2639-2648.
Chicago
Li, P., Guo, H., Wu, K., Su, L., Huang, K., Lai, R., Deng, Z., Li, S., Ouyang, P., Wang, Y., Chen, Z., Zhou, G., Wang, S."Decreased IL‑37 expression in hepatocellular carcinoma tissues and liver cancer cell lines". Oncology Letters 19, no. 4 (2020): 2639-2648. https://doi.org/10.3892/ol.2020.11393
Copy and paste a formatted citation
x
Spandidos Publications style
Li P, Guo H, Wu K, Su L, Huang K, Lai R, Deng Z, Li S, Ouyang P, Wang Y, Wang Y, et al: Decreased IL‑37 expression in hepatocellular carcinoma tissues and liver cancer cell lines. Oncol Lett 19: 2639-2648, 2020.
APA
Li, P., Guo, H., Wu, K., Su, L., Huang, K., Lai, R. ... Wang, S. (2020). Decreased IL‑37 expression in hepatocellular carcinoma tissues and liver cancer cell lines. Oncology Letters, 19, 2639-2648. https://doi.org/10.3892/ol.2020.11393
MLA
Li, P., Guo, H., Wu, K., Su, L., Huang, K., Lai, R., Deng, Z., Li, S., Ouyang, P., Wang, Y., Chen, Z., Zhou, G., Wang, S."Decreased IL‑37 expression in hepatocellular carcinoma tissues and liver cancer cell lines". Oncology Letters 19.4 (2020): 2639-2648.
Chicago
Li, P., Guo, H., Wu, K., Su, L., Huang, K., Lai, R., Deng, Z., Li, S., Ouyang, P., Wang, Y., Chen, Z., Zhou, G., Wang, S."Decreased IL‑37 expression in hepatocellular carcinoma tissues and liver cancer cell lines". Oncology Letters 19, no. 4 (2020): 2639-2648. https://doi.org/10.3892/ol.2020.11393
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team