Open Access

Effect of HBx on inflammation and mitochondrial oxidative stress in mouse hepatocytes

  • Authors:
    • Li‑Rong Ling
    • Dan‑Hua Zheng
    • Zhi‑Yang Zhang
    • Wen‑Hui Xie
    • Yue‑Hong Huang
    • Zhi‑Xin Chen
    • Xiao‑Zhong Wang
    • Dan Li
  • View Affiliations

  • Published online on: February 17, 2020     https://doi.org/10.3892/ol.2020.11404
  • Pages: 2861-2869
  • Copyright: © Ling et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hepatitis B virus x protein (HBx) serves an important role in the pathogenesis of the hepatitis B virus infection. Previous studies have reported that the interaction between HBx and hepatocyte mitochondria is an important factor leading to liver cell injury and apoptosis, ultimately inducing the formation of liver cancer. In the present study, a mouse model expressing HBx was constructed using hydrodynamic in vivo transfection based on the interaction between HBx and cytochrome c oxidase (COX) subunit III. The specific mechanism of HBx‑induced oxidative stress in mouse hepatocytes and the subsequent effect on mitochondrial function and inflammatory injury was assessed. The results demonstrated that HBx reduced the activity of COX and the expression of superoxide dismutase and upregulated the expression of malondialdehyde, NF‑κB and phospho‑AKT, thus increasing oxidative stress. In addition, HBx induced an increase in interleukin (IL)‑6, IL‑1β and IL‑18 expression levels, which created an inflammatory microenvironment in the liver, further promoting hepatocyte inflammatory injury. Therefore, it was proposed that HBx may affect hepatocyte mitochondrial respiration by reducing the activity of cytochrome c oxidase, leading to mitochondrial dysfunction and inducing hepatocyte inflammation and injury.
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April-2020
Volume 19 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Ling LR, Zheng DH, Zhang ZY, Xie WH, Huang YH, Chen ZX, Wang XZ and Li D: Effect of HBx on inflammation and mitochondrial oxidative stress in mouse hepatocytes. Oncol Lett 19: 2861-2869, 2020
APA
Ling, L., Zheng, D., Zhang, Z., Xie, W., Huang, Y., Chen, Z. ... Li, D. (2020). Effect of HBx on inflammation and mitochondrial oxidative stress in mouse hepatocytes. Oncology Letters, 19, 2861-2869. https://doi.org/10.3892/ol.2020.11404
MLA
Ling, L., Zheng, D., Zhang, Z., Xie, W., Huang, Y., Chen, Z., Wang, X., Li, D."Effect of HBx on inflammation and mitochondrial oxidative stress in mouse hepatocytes". Oncology Letters 19.4 (2020): 2861-2869.
Chicago
Ling, L., Zheng, D., Zhang, Z., Xie, W., Huang, Y., Chen, Z., Wang, X., Li, D."Effect of HBx on inflammation and mitochondrial oxidative stress in mouse hepatocytes". Oncology Letters 19, no. 4 (2020): 2861-2869. https://doi.org/10.3892/ol.2020.11404