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Profiling of serum metabolites in advanced colon cancer using liquid chromatography‑mass spectrometry

  • Authors:
    • Yang Zhang
    • Yechao Du
    • Zheyu Song
    • Suoning Liu
    • Wei Li
    • Daguang Wang
    • Jian Suo
  • View Affiliations / Copyright

    Affiliations: The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
  • Pages: 4002-4010
    |
    Published online on: April 3, 2020
       https://doi.org/10.3892/ol.2020.11510
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Abstract

Lymph node metastasis remains a key factor that affects the prognosis of patients with colon cancer. The aim of the present study was to identify and evaluate serum metabolites as biomarkers for the detection of tumor lymph node metastasis and the prediction of patient survival. The present study analyzed the metabolites in the serum of patients with advanced colon cancer both with and without lymph node metastasis. Blood samples from 104 patients with stage T3 colon cancer were collected and analyzed using liquid chromatography‑mass spectrometry. The metabolites were structurally confirmed with data from the Human Metabolome Database. The association between the serum metabolites and the clinicopathological characteristics and survival time of patients from the present study was analyzed. Overall, 227 different metabolites were identified in the serum of patients with stage T3 colon cancer with or without lymph node metastasis. Furthermore, 17 of these metabolites may potentially distinguish those patients with lymph node metastasis from those patients without. In addition, five factors, including abscisic acid, calcitroic acid and glucosylsphingosine presence in the serum, age and sex, were identified as independent predictors for lymph node metastasis (P<0.05). Furthermore, three factors, including abscisic acid, calcitroic acid and glucosylsphingosine presence in the serum were independent predictors for patient survival (P<0.05). In conclusion, the serum levels of abscisic acid, calcitroic‑acid and glucosylsphingosine may be considered as potential biomarkers to predict the occurrence of lymph node metastasis and the survival time of patients with colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Du Y, Song Z, Liu S, Li W, Wang D and Suo J: Profiling of serum metabolites in advanced colon cancer using liquid chromatography‑mass spectrometry. Oncol Lett 19: 4002-4010, 2020.
APA
Zhang, Y., Du, Y., Song, Z., Liu, S., Li, W., Wang, D., & Suo, J. (2020). Profiling of serum metabolites in advanced colon cancer using liquid chromatography‑mass spectrometry. Oncology Letters, 19, 4002-4010. https://doi.org/10.3892/ol.2020.11510
MLA
Zhang, Y., Du, Y., Song, Z., Liu, S., Li, W., Wang, D., Suo, J."Profiling of serum metabolites in advanced colon cancer using liquid chromatography‑mass spectrometry". Oncology Letters 19.6 (2020): 4002-4010.
Chicago
Zhang, Y., Du, Y., Song, Z., Liu, S., Li, W., Wang, D., Suo, J."Profiling of serum metabolites in advanced colon cancer using liquid chromatography‑mass spectrometry". Oncology Letters 19, no. 6 (2020): 4002-4010. https://doi.org/10.3892/ol.2020.11510
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Du Y, Song Z, Liu S, Li W, Wang D and Suo J: Profiling of serum metabolites in advanced colon cancer using liquid chromatography‑mass spectrometry. Oncol Lett 19: 4002-4010, 2020.
APA
Zhang, Y., Du, Y., Song, Z., Liu, S., Li, W., Wang, D., & Suo, J. (2020). Profiling of serum metabolites in advanced colon cancer using liquid chromatography‑mass spectrometry. Oncology Letters, 19, 4002-4010. https://doi.org/10.3892/ol.2020.11510
MLA
Zhang, Y., Du, Y., Song, Z., Liu, S., Li, W., Wang, D., Suo, J."Profiling of serum metabolites in advanced colon cancer using liquid chromatography‑mass spectrometry". Oncology Letters 19.6 (2020): 4002-4010.
Chicago
Zhang, Y., Du, Y., Song, Z., Liu, S., Li, W., Wang, D., Suo, J."Profiling of serum metabolites in advanced colon cancer using liquid chromatography‑mass spectrometry". Oncology Letters 19, no. 6 (2020): 4002-4010. https://doi.org/10.3892/ol.2020.11510
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