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Article

Detection of circulating tumor cells in patients with laryngeal cancer using ScreenCell: Comparative pre- and post-operative analysis and association with prognosis

  • Authors:
    • Maria Ida Rizzo
    • Massimo Ralli
    • Chiara Nicolazzo
    • Angela Gradilone
    • Raffaella Carletti
    • Cira Di Gioia
    • Marco De Vincentiis
    • Antonio Greco
  • View Affiliations / Copyright

    Affiliations: Department of Surgical Science, Sapienza University of Rome, Rome 00186, Italy, Department of Sense Organs, Sapienza University of Rome, Rome 00186, Italy, Department of Molecular Medicine-Circulating Tumor Cells Unit, Sapienza University of Rome, Rome 00186, Italy, Department of Radiological, Oncological and Pathological Sciences, Sapienza University of Rome, Rome 00186, Italy, Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, Rome 00186, Italy
  • Pages: 4183-4188
    |
    Published online on: April 10, 2020
       https://doi.org/10.3892/ol.2020.11528
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Abstract

The presence of circulating tumor cells (CTCs) in the blood of patients with metastatic breast, colorectal and prostate cancer have been widely investigated; however, few studies have examined CTCs in patients with laryngeal cancer. The present pilot study aimed to detect pre‑ and postoperative CTCs in the blood of patients with laryngeal cancer and evaluate the association with prognosis. Eight patients with laryngeal squamous cell carcinoma (LSCC) at stage III were included in the present study and underwent total or subtotal laryngectomy and radical bilateral neck lymph node dissection. Blood samples were collected from all patients before and after surgery at different time‑points. The following processing steps were followed; preoperative blood sampling, surgery, postoperative blood sampling at 3, 6 and 12 month follow‑ups, and prognostic association analysis. CTCs were retained on ScreenCell filters for cytological characterization. The presence of CTCs was associated with a less favorable prognosis, whereas a decrease of CTCs in the postoperative sampling was observed in patients who exhibited an improved therapeutic response. The results of the present pilot study revealed a possible association between the presence of CTCs and a less favorable prognosis in patients with LSCC; therefore, these preliminary findings may encourage further research into the incorporation of a liquid biopsy in the management of LSCC, as this may help identify patients with occult metastatic disease earlier and in a non‑invasive manner. In addition, this approach may represent novel independent prognostic factor for use in the clinical evaluation of patients with LSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Rizzo MI, Ralli M, Nicolazzo C, Gradilone A, Carletti R, Gioia CD, De Vincentiis M and Greco A: Detection of circulating tumor cells in patients with laryngeal cancer using ScreenCell: Comparative pre- and post-operative analysis and association with prognosis. Oncol Lett 19: 4183-4188, 2020.
APA
Rizzo, M.I., Ralli, M., Nicolazzo, C., Gradilone, A., Carletti, R., Gioia, C.D. ... Greco, A. (2020). Detection of circulating tumor cells in patients with laryngeal cancer using ScreenCell: Comparative pre- and post-operative analysis and association with prognosis. Oncology Letters, 19, 4183-4188. https://doi.org/10.3892/ol.2020.11528
MLA
Rizzo, M. I., Ralli, M., Nicolazzo, C., Gradilone, A., Carletti, R., Gioia, C. D., De Vincentiis, M., Greco, A."Detection of circulating tumor cells in patients with laryngeal cancer using ScreenCell: Comparative pre- and post-operative analysis and association with prognosis". Oncology Letters 19.6 (2020): 4183-4188.
Chicago
Rizzo, M. I., Ralli, M., Nicolazzo, C., Gradilone, A., Carletti, R., Gioia, C. D., De Vincentiis, M., Greco, A."Detection of circulating tumor cells in patients with laryngeal cancer using ScreenCell: Comparative pre- and post-operative analysis and association with prognosis". Oncology Letters 19, no. 6 (2020): 4183-4188. https://doi.org/10.3892/ol.2020.11528
Copy and paste a formatted citation
x
Spandidos Publications style
Rizzo MI, Ralli M, Nicolazzo C, Gradilone A, Carletti R, Gioia CD, De Vincentiis M and Greco A: Detection of circulating tumor cells in patients with laryngeal cancer using ScreenCell: Comparative pre- and post-operative analysis and association with prognosis. Oncol Lett 19: 4183-4188, 2020.
APA
Rizzo, M.I., Ralli, M., Nicolazzo, C., Gradilone, A., Carletti, R., Gioia, C.D. ... Greco, A. (2020). Detection of circulating tumor cells in patients with laryngeal cancer using ScreenCell: Comparative pre- and post-operative analysis and association with prognosis. Oncology Letters, 19, 4183-4188. https://doi.org/10.3892/ol.2020.11528
MLA
Rizzo, M. I., Ralli, M., Nicolazzo, C., Gradilone, A., Carletti, R., Gioia, C. D., De Vincentiis, M., Greco, A."Detection of circulating tumor cells in patients with laryngeal cancer using ScreenCell: Comparative pre- and post-operative analysis and association with prognosis". Oncology Letters 19.6 (2020): 4183-4188.
Chicago
Rizzo, M. I., Ralli, M., Nicolazzo, C., Gradilone, A., Carletti, R., Gioia, C. D., De Vincentiis, M., Greco, A."Detection of circulating tumor cells in patients with laryngeal cancer using ScreenCell: Comparative pre- and post-operative analysis and association with prognosis". Oncology Letters 19, no. 6 (2020): 4183-4188. https://doi.org/10.3892/ol.2020.11528
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