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Article Open Access

Significant diagnostic value of circulating tumour cells in colorectal cancer

  • Authors:
    • Haijiao Yu
    • Ling Ma
    • Yubing Zhu
    • Wenxia Li
    • Lei Ding
    • Hong Gao
  • View Affiliations / Copyright

    Affiliations: Department of Colorectal Tumour Surgery, Beijing Shijitan Hospital Affiliated to Capital Medical University, Beijing 100038, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 317-325
    |
    Published online on: April 15, 2020
       https://doi.org/10.3892/ol.2020.11537
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Abstract

Circulating tumour cells (CTCs) have potential utility in various clinical applications for cancer management. The present study focused on evaluating the diagnostic role of CTCs in colorectal cancer (CRC). A total of 89 blood samples from 59 patients diagnosed with CRC and 30 healthy individuals were collected for CTC detection. The Cyttel method is an improved CTC detection strategy, which combines negative enrichment with immunofluorescence and fluorescence in situ hybridization. This method effectively detected a significant increase in total CTCs in patients with CRC (49/59) compared with those in healthy controls (3/30). A cut‑off value of 2 CTCs/3.2 ml blood yielded a sensitivity of 83.05% and a specificity of 100%. Additionally, three traditional serum tumour markers, namely carcinoembryonic antigen (CEA), cancer antigen 19‑9 (CA19‑9) and CA72‑4, were examined by immunoassays. The diagnostic sensitivity of CTCs was much higher than that of CEA, CA19‑9 and CA72‑4 alone or in combination, particularly in patients with early stage CRC. The combined sensitivity of CTCs and CEA reached 91.53%, which was only slightly lower than the sensitivity of all four markers combined (CTCs + CEA + CA19‑9 + CA72‑4). CTCs with aneuploidy of chromosome 7 or 8 were carefully distinguished, and the associations among different types of CTCs, clinicopathological characteristics and overall survival were statistically analysed. Total CTCs were revealed to be significantly associated with tumour differentiation and nerve invasion. CTCs were more likely to be detected in poorly differentiated CRC tumours than in well‑ and moderately‑differentiated tumours (P=0.026). Furthermore, to the best of our knowledge, the present study was the first to report that CTCs with multiploidy of chromosome 7 were significantly associated with TNM stage. These CTCs exhibited a high chance of being identified in the peripheral blood of patients with late‑stage CRC (stage III‑IV; P=0.031). The present study suggests that the combination of CTCs and CEA may serve as an effective potential diagnostic and prognostic indicator in patients with CRC. Detection of CTCs with aneuploidy may have increased specificity in predicting highly malignant and invasive tumours in CRC management.
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1 

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 136:E359–E386. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2018. CA Cancer J Clin. 68:7–30. 2018. View Article : Google Scholar : PubMed/NCBI

3 

Kekelidze M, D'Errico L, Pansini M, Tyndall A and Hohmann J: Colorectal cancer: Current imaging methods and future perspectives for the diagnosis, staging and therapeutic response evaluation. World J Gastroenterol. 19:8502–8514. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Ponz de Leon M and Di Gregorio C: Pathology of colorectal cance. Dig Liver Dis. 33:372–388. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Shah R, Jones E, Vidart V, Kuppen PJ, Conti JA and Francis NK: Biomarkers for early detection of colorectal cancer and polyps: Systematic review. Cancer Epidemiol Biomarkers Prev. 23:1712–1728. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Ryuk JP, Choi GS, Park JS, Kim HJ, Park SY, Yoon GS, Jun SH and Kwon YC: Predictive factors and the prognosis of recurrence of colorectal cancer within 2 years after curative resection. Ann Surg Treat Res. 86:143–151. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Masuda T, Hayashi N, Iguchi T, Ito S, Eguchi H and Mimori K: Clinical and biological significance of circulating tumor cells in cancer. Mol Oncol. 10:408–417. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Gkountela S, Castro-Giner F, Szczerba BM, Vetter M, Landin J, Scherrer R, Krol I, Scheidmann MC, Beisel C, Stirnimann CU, et al: Circulating tumor cell clustering shapes DNA methylation to enable metastasis seeding. Cell. 176:98–112.e14. 2019. View Article : Google Scholar : PubMed/NCBI

9 

Massague J and Obenauf AC: Metastatic colonization by circulating tumour cells. Nature. 529:298–306. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Bork U, Rahbari NN, Schölch S, Reissfelder C, Kahlert C, Büchler MW, Weitz J and Koch M: Circulating tumour cells and outcome in non-metastatic colorectal cancer: A prospective study. Br J Cancer. 112:1306–1313. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Cohen SJ, Punt CJA, Iannotti N, Saidman BH, Sabbath KD, Gabrail NY, Picus J, Morse MA, Mitchell E, Miller MC, et al: Prognostic significance of circulating tumor cells in patients with metastatic colorectal cancer. Ann Oncol. 20:1223–1229. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Krebs MG, Metcalf RL, Carter L, Brady G, Blackhall FH and Dive C: Molecular analysis of circulating tumour cells-biology and biomarkers. Nat Rev Clin Oncol. 11:129–144. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Yu M, Bardia A, Wittner BS, Stott SL, Smas ME, Ting DT, Isakoff SJ, Ciciliano JC, Wells MN, Shah AM, et al: Circulating breast tumor cells exhibit dynamic changes in epithelial and mesenchymal composition. Science. 339:580–584. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Zhang L, Zhao M and He S: Study on the clinical significance of detection of Cyttel circulating tumor cells in peripheral blood of patients with gastric cancer. J Clin Exp Med. 7:772–774. 2018.

15 

Rajagopalan H: Aneuploidy and cancer. Nature. 432:338–341. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Sayagués JM, Abad MM, Melchor HB, Gutiérrez ML, González-González M, Jensen E, Bengoechea O, Fonseca E, Orfao A and Muñoz-Bellvis L: Intratumoural cytogenetic heterogeneity of sporadic colorectal carcinomas suggests several pathways to liver metastasis. J Pathol. 221:308–319. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Provenzale D, Gupta S, Ahnen DJ, Markowitz AJ, Chung DC, Mayer RJ, Regenbogen SE, Blanco AM, Bray T, Cooper G, et al: NCCN guidelines insights: Colorectal cancer screening, Version 1.2018. J Natl Compr Canc Netw. 16:939–949. 2018. View Article : Google Scholar : PubMed/NCBI

18 

Yang Y, Li J, Jin L, Wang D, Zhang J, Wang J, Zhao X, Wu G, Yao H and Zhang Z: Independent correlation between Ki67 index and circulating tumor cells in the diagnosis of colorectal cancer. Anticancer Res. 37:4693–4700. 2017.PubMed/NCBI

19 

Gorges TM, Stein A, Quidde J, Hauch S, Röck K, Riethdorf S, Joosse SA and Pantel K: Improved detection of circulating tumor cells in metastatic colorectal cancer by the combination of the CellSearch® system and the AdnaTest®. PLoS One. 11:e1551262016. View Article : Google Scholar

20 

Chen F, Wang S, Fang Y, Zheng L, Zhi X, Cheng B, Chen Y, Zhang C, Shi D, Song H, et al: Feasibility of a novel one-stop ISET device to capture CTCs and its clinical application. Oncotarget. 8:3029–3041. 2017.PubMed/NCBI

21 

Chen CJ, Sung WW, Chen HC, Chern JY, Hsu TH, Min Lin Y, Lin SH, Peck K and Yeh TK: Early assessment of colorectal cancer by quantifying circulating tumor cells in peripheral blood: ECT2 in diagnosis of colorectal cancer. Int J Mol Sci. 18:7432017. View Article : Google Scholar

22 

Chen YY and Xu GB: Effect of circulating tumor cells combined with negative enrichment and CD45-FISH identification in diagnosis, therapy monitoring and prognosis of primary lung cancer. Med Oncol. 31:2402014. View Article : Google Scholar : PubMed/NCBI

23 

Ning N, Zhan T, Zhang Y, Chen Q, Feng F, Yang Z, Liu Z, Xu D, Wang F, Guo Y, et al: Improvement of specific detection of circulating tumor cells using combined CD45 staining and fluorescence in situ hybridization. Clin Chim Acta. 433:69–75. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Sun B, Liu H, Wang S, Xiang J and Liu X: Prognostic impact of circulating tumor cells in patients with ampullary cancer. J Cell Physiol. 233:5014–5022. 2018. View Article : Google Scholar : PubMed/NCBI

25 

Miller MC, Doyle GV and Terstappen LW: Significance of circulating tumor cells detected by the CellSearch system in patients with metastatic breast, colorectal and prostate cancer. J Oncol. 2010:6174212010. View Article : Google Scholar : PubMed/NCBI

26 

Ilie M, Hofman V, Long-Mira E, et al: Clinical and pathological characteristics of CTC-positive COPD patients). PLoS One. 9:e1115972014. View Article : Google Scholar : PubMed/NCBI

27 

Meyerhardt JA, Mangu PB, Flynn PJ, Korde L, Loprinzi CL, Minsky BD, Petrelli NJ, Ryan K, Schrag DH, Wong SL, et al: Follow-up care, surveillance protocol, and secondary prevention measures for survivors of colorectal cancer: American Society of Clinical Oncology clinical practice guideline endorsement. J Clin Oncol. 31:4465–4470. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Abe S, Kawai K, Ishihara S, Nozawa H, Hata K, Kiyomatsu T, Tanaka T and Watanabe T: Prognostic impact of carcinoembryonic antigen and carbohydrate antigen 19-9 in stage IV colorectal cancer patients after R0 resection. J Surg Res. 205:384–392. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Li Q, Dai W, Li Y, Xu Y, Li X and Cai S: Nomograms for predicting the prognostic value of serological tumor biomarkers in colorectal cancer patients after radical resection. Sci Rep. 7:463452017. View Article : Google Scholar : PubMed/NCBI

30 

Gao Y, Wang J, Zhou Y, Sheng S, Qian SY and Huo X: Evaluation of serum CEA, CA19-9, CA72-4, CA125 and ferritin as diagnostic markers and factors of clinical parameters for colorectal cancer. Sci Rep. 8:27322018. View Article : Google Scholar : PubMed/NCBI

31 

Davoli T, Uno H, Wooten EC and Elledge SJ: Tumor aneuploidy correlates with markers of immune evasion and with reduced response to immunotherapy. Science. 355(pii): eaaf83992017. View Article : Google Scholar : PubMed/NCBI

32 

Papazachariou I, Tsiambas E, Koliopoulou A, Tsounis D, Manaios M, Fotiades PP, Karameris A and Patsouris E: Chromosomes 7, 16 numerical aberrations are poor prognostic factors in colorectal adenocarcinoma: A tissue microarray analysis. Basic App Pathol. 1:125–130. 2008. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Yu H, Ma L, Zhu Y, Li W, Ding L and Gao H: Significant diagnostic value of circulating tumour cells in colorectal cancer. Oncol Lett 20: 317-325, 2020.
APA
Yu, H., Ma, L., Zhu, Y., Li, W., Ding, L., & Gao, H. (2020). Significant diagnostic value of circulating tumour cells in colorectal cancer. Oncology Letters, 20, 317-325. https://doi.org/10.3892/ol.2020.11537
MLA
Yu, H., Ma, L., Zhu, Y., Li, W., Ding, L., Gao, H."Significant diagnostic value of circulating tumour cells in colorectal cancer". Oncology Letters 20.1 (2020): 317-325.
Chicago
Yu, H., Ma, L., Zhu, Y., Li, W., Ding, L., Gao, H."Significant diagnostic value of circulating tumour cells in colorectal cancer". Oncology Letters 20, no. 1 (2020): 317-325. https://doi.org/10.3892/ol.2020.11537
Copy and paste a formatted citation
x
Spandidos Publications style
Yu H, Ma L, Zhu Y, Li W, Ding L and Gao H: Significant diagnostic value of circulating tumour cells in colorectal cancer. Oncol Lett 20: 317-325, 2020.
APA
Yu, H., Ma, L., Zhu, Y., Li, W., Ding, L., & Gao, H. (2020). Significant diagnostic value of circulating tumour cells in colorectal cancer. Oncology Letters, 20, 317-325. https://doi.org/10.3892/ol.2020.11537
MLA
Yu, H., Ma, L., Zhu, Y., Li, W., Ding, L., Gao, H."Significant diagnostic value of circulating tumour cells in colorectal cancer". Oncology Letters 20.1 (2020): 317-325.
Chicago
Yu, H., Ma, L., Zhu, Y., Li, W., Ding, L., Gao, H."Significant diagnostic value of circulating tumour cells in colorectal cancer". Oncology Letters 20, no. 1 (2020): 317-325. https://doi.org/10.3892/ol.2020.11537
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