Open Access

STAT1 is a modulator of the expression of frequently rearranged in advanced T‑cell lymphomas 1 expression in U251 cells

  • Authors:
    • Geng Guo
    • Shule Wang
    • Yining Hao
    • Yeqing Ren
    • Yongqiang Wu
    • Jianping Zhang
    • Dong Liu
  • View Affiliations

  • Published online on: April 21, 2020     https://doi.org/10.3892/ol.2020.11555
  • Pages: 248-256
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Aberrant expression of frequently rearranged in advanced T‑cell lymphomas 1 (FRAT1) contributes to poor prognosis in a number of carcinomas. However, its role in glioma remains controversial. In the present study, gene expression profiling was performed using Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO) functional enrichment and ingenuity pathway analysis (IPA) to evaluate the differential expression of genes and proteins in FRAT1 knockdown U251 glioma cells in comparison with the control. Western blot analysis was conducted to assess the expression levels of FRAT1 and STAT1. A total of 895 downregulated genes were identified in FRAT1‑silenced U251 cells. The most enriched processes determined by GO and KEGG analysis of the 895 differentially expressed genes were associated with proliferation, migration and invasion. According to IPA, significant canonical pathways, including the interferon, hepatic fibrosis and Wnt/β‑catenin signaling pathways, were identified to be the major enriched pathways. The elevated expression of STAT1 in U251 cells was validated. These results highlighted the regulatory role of FRAT1 in glioma cells with upregulated STAT1 expression.

Related Articles

Journal Cover

July 2020
Volume 20 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Guo, G., Wang, S., Hao, Y., Ren, Y., Wu, Y., Zhang, J., & Liu, D. (2020). STAT1 is a modulator of the expression of frequently rearranged in advanced T‑cell lymphomas 1 expression in U251 cells. Oncology Letters, 20, 248-256. https://doi.org/10.3892/ol.2020.11555
MLA
Guo, G., Wang, S., Hao, Y., Ren, Y., Wu, Y., Zhang, J., Liu, D."STAT1 is a modulator of the expression of frequently rearranged in advanced T‑cell lymphomas 1 expression in U251 cells". Oncology Letters 20.1 (2020): 248-256.
Chicago
Guo, G., Wang, S., Hao, Y., Ren, Y., Wu, Y., Zhang, J., Liu, D."STAT1 is a modulator of the expression of frequently rearranged in advanced T‑cell lymphomas 1 expression in U251 cells". Oncology Letters 20, no. 1 (2020): 248-256. https://doi.org/10.3892/ol.2020.11555